##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779849_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187991
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.085844535110724	34.0	23.0	34.0	12.0	34.0
2	28.745854854753684	34.0	23.0	34.0	12.0	34.0
3	28.472783271539594	34.0	23.0	34.0	11.0	34.0
4	28.89767063316861	34.0	23.0	34.0	12.0	34.0
5	29.11769712379848	34.0	27.0	34.0	11.0	34.0
6	31.473474794006098	37.0	27.0	38.0	12.0	38.0
7	31.604832146219767	37.0	27.0	38.0	12.0	38.0
8	30.984658840050855	37.0	26.0	38.0	11.0	38.0
9	32.035012314419305	37.0	31.0	38.0	12.0	38.0
10-11	30.633142012117602	36.5	25.5	38.0	11.0	38.0
12-13	31.436691118191828	37.0	27.0	38.0	11.0	38.0
14-15	30.29868983089616	36.5	24.5	38.0	11.0	38.0
16-17	30.88081344319675	36.5	25.0	38.0	11.0	38.0
18-19	31.642278087780795	37.0	27.5	38.0	11.0	38.0
20-21	30.483384842891414	36.5	25.0	38.0	11.0	38.0
22-23	31.311480336824637	37.0	27.0	38.0	11.0	38.0
24-25	31.601954348878404	37.0	27.5	38.0	11.0	38.0
26-27	30.51864184987579	37.0	24.5	38.0	11.0	38.0
28-29	31.680069790575082	37.0	29.0	38.0	11.0	38.0
30-31	32.09334755387226	37.5	31.0	38.0	11.0	38.0
32-33	32.19498539823715	38.0	31.0	38.0	11.0	38.0
34-35	32.15685059391141	37.5	31.0	38.0	11.0	38.0
36-37	31.675965870706577	37.0	28.0	38.0	11.0	38.0
38-39	31.58556526642233	37.0	28.0	38.0	11.0	38.0
40-41	31.539903506018906	37.0	27.0	38.0	11.0	38.0
42-43	31.940885467921337	37.0	29.0	38.0	11.0	38.0
44-45	32.00286981823598	37.0	31.0	38.0	11.0	38.0
46-47	31.876932938278962	37.0	29.0	38.0	11.0	38.0
48-49	31.582272555601065	37.0	27.0	38.0	11.0	38.0
50-51	30.96113111797905	37.0	25.5	38.0	11.0	38.0
52-53	31.501968179327733	37.0	27.0	38.0	11.0	38.0
54-55	31.63663154087164	37.0	28.0	38.0	11.0	38.0
56-57	31.68913671399163	37.0	27.0	38.0	11.0	38.0
58-59	31.939193897580203	37.0	30.0	38.0	11.0	38.0
60-61	31.80745088860637	37.0	27.5	38.0	11.0	38.0
62-63	31.77318595039124	37.0	27.0	38.0	11.0	38.0
64-65	31.800054257916603	37.0	28.5	38.0	11.0	38.0
66-67	31.7025283125256	37.0	27.0	38.0	11.0	38.0
68-69	31.078176614837943	37.0	25.5	38.0	11.0	38.0
70-71	31.11100797378598	37.0	26.0	38.0	11.0	38.0
72-73	31.251921634546335	37.0	27.0	38.0	11.0	38.0
74-75	31.464750972121006	37.0	27.0	38.0	11.0	38.0
76	30.310456351633857	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	57.0
13	576.0
14	2062.0
15	3620.0
16	5102.0
17	5878.0
18	5885.0
19	5290.0
20	4316.0
21	3237.0
22	2664.0
23	2377.0
24	2250.0
25	2297.0
26	2427.0
27	2673.0
28	3188.0
29	3689.0
30	4395.0
31	5452.0
32	6742.0
33	8521.0
34	11694.0
35	16722.0
36	27808.0
37	49068.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.0996962620551	22.753216909320127	9.098307897718508	30.048778930906266
2	19.78286194551867	20.86589251613109	29.012559111872378	30.338686426477864
3	20.832380273523732	20.323313350107185	29.880685777510624	28.96362059885846
4	14.224085195567874	22.167018633870768	30.12644222329792	33.48245394726344
5	17.907772180583112	30.60731630769558	31.944082429478005	19.5408290822433
6	27.24269634231972	35.69855522692938	15.975487797093432	21.083260633657467
7	26.815580049365906	27.186888245808156	20.284705081283512	25.71282662354243
8	23.58911615288454	34.483070457749285	16.77953028167141	25.148283107694763
9	24.117862975513482	17.324091264542695	17.549645974859164	41.00839978508466
10-11	23.101331475048806	25.86428848803375	23.02685824019746	28.007521796719985
12-13	23.430376986132316	21.823917102414477	24.859966700533537	29.88573921091967
14-15	25.329404067215982	24.016841231761095	21.283997638184807	29.369757062838115
16-17	26.496747184705647	24.54266427648132	21.619386034437817	27.34120250437521
18-19	22.94109198459476	24.652637402387388	23.979456135498012	28.42681447751984
20-21	24.850657744253716	23.980137347000653	23.33542563207813	27.833779276667496
22-23	26.44929463582782	23.490329169947017	22.27482605272677	27.785550141498394
24-25	22.993488946102943	27.076196352958696	21.76335723556823	28.16695746537013
26-27	24.304750252673017	27.067397201978828	23.211872971966592	25.415979573381563
28-29	22.957534811347102	26.535090635847375	24.324187735234272	26.18318681757125
30-31	23.907591075080923	25.6278907710762	24.324101483851575	26.1404166699913
32-33	21.89838875265305	26.98613231484486	24.30754663787096	26.807932294631126
34-35	22.117428020480084	27.79360329809163	24.07500498703371	26.013963694394576
36-37	24.87523808510412	25.084859383479284	23.728971365943455	26.31093116547314
38-39	24.791594626945074	24.45325176220242	24.98869530522676	25.76645830562575
40-41	22.711580403212935	25.699505292834722	24.821001117080694	26.767913186871645
42-43	21.04994388029214	25.37754466969163	25.486060502896414	28.086450947119808
44-45	20.71112624246277	23.84225211255243	27.213026675213513	28.233594969771282
46-47	21.338748543797774	24.92220289485023	26.459792860220542	27.27925570113145
48-49	21.452622731939293	25.3442983972637	25.917464133921303	27.2856147368757
50-51	23.87386428920995	25.288050301083047	23.984775623976	26.853309785731007
52-53	19.131533448596887	26.67641973666711	25.801037371990954	28.391009442745048
54-55	20.031439180347054	25.302688555287205	25.291783255843647	29.37408900852209
56-57	20.97386844849233	25.96610133424127	24.68798544464069	28.37204477262571
58-59	20.356809409937146	25.632875196505857	25.13838534033085	28.87193005322615
60-61	19.631503620585995	24.889733071565765	25.19858688076274	30.280176427085493
62-63	20.70781630291665	25.23587226854992	24.689249757278326	29.3670616712551
64-65	21.257086005687427	24.464974981179356	24.21092954029746	30.06700947283576
66-67	20.0901775620137	27.403338431868058	22.699208618740442	29.807275387377803
68-69	20.908979685197696	28.558282045417066	22.032969663441335	28.499768605943903
70-71	20.307463162934198	28.536092345337515	23.08979201021331	28.066652481514975
72-73	20.909381067638375	27.480118094528823	23.93196265659494	27.678538181237865
74-75	21.02991049460707	27.647989786011625	22.62983601760849	28.69226370177282
76	21.81640219802433	27.349231595801836	21.808954874539197	29.025411331634633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.5
14	4.5
15	18.0
16	29.0
17	30.0
18	29.5
19	41.0
20	86.0
21	119.0
22	178.0
23	259.0
24	355.5
25	430.0
26	520.0
27	673.5
28	880.0
29	1023.0
30	1214.0
31	1660.0
32	2156.5
33	2398.0
34	2671.0
35	3239.5
36	3929.0
37	4323.0
38	4808.5
39	5707.5
40	6586.0
41	7461.0
42	7871.0
43	8208.5
44	8993.5
45	9629.5
46	9818.0
47	9788.5
48	9689.0
49	9538.0
50	9457.0
51	9315.0
52	8702.5
53	7952.0
54	7672.0
55	7447.5
56	6989.5
57	6409.0
58	6062.0
59	5637.0
60	4835.0
61	4156.5
62	3855.0
63	3703.5
64	3305.0
65	2857.5
66	2466.5
67	2276.0
68	2221.0
69	2007.0
70	1728.0
71	1608.0
72	1497.5
73	1353.5
74	1236.5
75	1153.0
76	1088.0
77	974.5
78	843.5
79	761.0
80	682.5
81	537.5
82	437.5
83	404.0
84	335.0
85	250.5
86	191.5
87	148.0
88	135.5
89	99.5
90	63.0
91	46.5
92	43.0
93	38.0
94	30.5
95	22.5
96	17.0
97	11.0
98	9.5
99	13.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0015958210765408983
7	0.0037235825119287624
8	0.0031916421530817965
9	0.0053194035884696605
10-11	0.0021277614353878644
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0015958210765408983
20-21	0.0
22-23	0.0015958210765408983
24-25	0.0015958210765408983
26-27	5.319403588469661E-4
28-29	0.007181194844434042
30-31	2.6597017942348305E-4
32-33	0.0
34-35	0.0018617912559643812
36-37	0.01755403184194988
38-39	0.008511045741551457
40-41	5.319403588469661E-4
42-43	0.0
44-45	0.003989552691352246
46-47	0.0010638807176939322
48-49	0.0
50-51	0.0015958210765408983
52-53	0.008511045741551457
54-55	0.004787463229622695
56-57	0.010106866818092355
58-59	0.010372836997515839
60-61	0.02021373363618471
62-63	0.009840896638668873
64-65	0.017288061662526395
66-67	0.015160300227138533
68-69	0.0
70-71	5.319403588469661E-4
72-73	0.0031916421530817965
74-75	0.007181194844434042
76	0.0021277614353878644
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	187991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.98446699741679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4585069867829	48.92156816967571
2	9.7815068725553	12.517290073440401
3	5.654301976008956	10.853624945321206
4	3.691497685094667	9.447940472119209
5	2.020131765222534	6.462852713115727
6	1.053015907828374	4.042599696131968
7	0.5560758002001723	2.4906149580179044
8	0.26066843126081907	1.3342984509821028
9	0.1843465529172578	1.0615784338099647
>10	0.3399480221290277	2.867632087385831
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008511045741551457	0.0
2	0.0	0.0	0.0	0.008511045741551457	0.0
3	0.0	0.0	0.0	0.008511045741551457	0.0
4	0.0	0.0	0.0	0.01489433004771505	0.0
5	0.0	0.0	0.0	0.015426270406562016	0.0
6	0.0	0.0	0.0	0.015426270406562016	0.0
7	0.0	0.0	0.0	0.01755403184194988	0.0
8	0.0	0.0	0.0	0.01755403184194988	0.0
9	0.0	0.0	0.0	0.018085972200796847	0.0
10	0.0	0.0	0.0	0.021277614353878642	0.0
11	0.0	0.0	0.0	0.02287343543041954	0.0
12	0.0	0.0	0.0	0.02287343543041954	0.0
13	0.0	0.0	0.0	0.02340537578926651	0.0
14	0.0	0.0	0.0	0.02446925650696044	0.0
15	0.0	0.0	0.0	0.02446925650696044	0.0
16	0.0	0.0	0.0	0.02446925650696044	0.0
17	0.0	0.0	0.0	0.027660898660042236	0.0
18	0.0	0.0	0.0	0.028192839018889203	0.0
19	0.0	0.0	0.0	0.028192839018889203	0.0
20	0.0	0.0	0.0	0.0297886600954301	0.0
21	0.0	0.0	0.0	0.032448361889664934	0.0
22	0.0	0.0	0.0	0.04681075157853302	0.0
23	0.0	0.0	0.0	0.05000239373161481	0.0
24	0.0	0.0	0.0	0.05000239373161481	0.0
25	0.0	0.0	0.0	0.05266209552584964	0.0
26	0.0	0.0	0.0	0.05691761839662537	0.0
27	0.0	0.0	0.0	0.08298269598012671	0.0
28	0.0	0.0	0.0	0.09894090674553568	0.0
29	0.0	0.0	0.0	0.13724061258251724	0.0
30	0.0	0.0	0.0	0.16490151124255947	0.0
31	0.0	0.0	0.0	0.19947763456761228	0.0
32	0.0	0.0	0.0	0.24096898255767563	0.0
33	0.0	0.0	0.0	0.29895048167199495	0.0
34	0.0	0.0	0.0	0.37235825119287624	0.0
35	0.0	0.0	0.0	0.4643839332734014	0.0
36	0.0	0.0	0.0	0.6244979812863382	0.0
37	0.0	0.0	0.0	0.9787702602784176	0.0
38	0.0	0.0	0.0	1.348468809677059	0.0
39	0.0	0.0	0.0	1.8564718523759116	0.0
40	0.0	0.0	0.0	2.369794298663234	0.0
41	0.0	0.0	0.0	2.980461830619551	0.0
42	0.0	0.0	0.0	3.7081562415222007	0.0
43	0.0	0.0	0.0	4.343293029985478	0.0
44	0.0	0.0	0.0	5.009814299620727	0.0
45	0.0	0.0	0.0	5.724742141911049	0.0
46	0.0	0.0	0.0	6.412541025900176	0.0
47	0.0	0.0	0.0	7.1147022995781715	0.0
48	0.0	0.0	0.0	7.687602066056353	0.0
49	0.0	0.0	0.0	8.313695868419233	0.0
50	0.0	0.0	0.0	8.928086982887478	0.0
51	0.0	0.0	0.0	9.577586161039624	0.0
52	0.0	0.0	0.0	10.222829816320994	0.0
53	0.0	0.0	0.0	10.90211765456857	0.0
54	0.0	0.0	0.0	11.52980727800799	0.0
55	0.0	0.0	0.0	12.144730332835083	0.0
56	0.0	0.0	0.0	12.775611598427584	0.0
57	0.0	0.0	0.0	13.306488076556857	0.0
58	0.0	0.0	0.0	13.867685155140405	0.0
59	0.0	0.0	0.0	14.275151470017182	0.0
60	0.0	0.0	0.0	14.744854806879053	0.0
61	0.0	0.0	0.0	15.17838619933933	0.0
62	0.0	0.0	0.0	15.626279981488475	0.0
63	0.0	0.0	0.0	16.131623322393093	0.0
64	0.0	0.0	0.0	16.617284870020374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGT	75	0.0	56.004898	46
GCCGTAA	25	2.3848431E-4	56.004898	54
CAACGGG	25	2.3911255E-4	55.9751	20
AGCGTAA	20	0.006933327	52.51857	35
GTCCGCA	20	0.006940632	52.50459	56
ATTGCGA	20	0.006940632	52.50459	48
TTCGGTA	20	0.006947943	52.49062	26
GACGAAT	40	7.4597665E-7	52.49062	23
TCGCCGT	75	0.0	51.337822	52
GCGATCT	35	2.0829517E-5	49.991066	28
ACGAGCG	35	2.0829517E-5	49.991066	24
ATCCCGG	30	5.864255E-4	46.670746	42
CGACGAA	205	0.0	46.07706	22
GACGACC	55	1.3066165E-7	44.537495	23
CGCCGTA	90	1.8189894E-12	42.781517	53
GGTGGTC	100	0.0	42.003674	47
GCGTAGA	35	0.0012509114	40.01415	36
ACACGTA	35	0.0012541916	39.992855	10
GACGGTG	35	0.0012558344	39.982212	14
GATCTCG	80	1.4097168E-9	39.37844	41
>>END_MODULE