FastQCFastQC Report
Wed 25 May 2016
SRR1779849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187991
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2584013.745338872605602No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG32591.7335936294822625No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC18010.9580245862833859No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT7010.3728901915517232TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6090.3239516785378023No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT3700.19681793277337745No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT3570.18990270810836687No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2630.13990031437675207No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2000.10638807176939322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA150.002206984470.11973651
GCCGTAT150.002213997570.0636846
CATCGCA150.002228073869.951859
CGTATAT308.387096E-658.2932134
CACGGAT308.387096E-658.2932132
ATATCGT308.387096E-658.2932137
ACGTATA308.387096E-658.2932133
TATCGTA308.387096E-658.2932138
AACCGAG252.3940025E-455.9614823
ATCTCGT36750.054.34354437
TCTCGTA36650.054.30095738
GTATCTC35900.054.2662735
CGTATCT35950.054.09350234
TATCTCG36150.054.08448836
CGTCTTC37950.054.0016548
CGTATGC38800.053.90619741
TCGTATG38950.053.87818540
CCGTCTT38050.053.85973447
TATGCCG38550.053.8021343
GCCGTCT38250.053.76128446