FastQCFastQC Report
Wed 25 May 2016
SRR1779848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55184
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGT150.002213659270.0000133
AGATATC150.002213659270.0000140
TCATTAA150.002213659270.0000160
TTTGTAG150.002213659270.0000140
ATGCTCG150.002213659270.0000125
TAGCACG150.002213659270.000011
CTAGTGT150.002213659270.0000133
GATGGAC150.002213659270.0000123
TTACGTG150.002213659270.0000132
GCGTGAC150.002213659270.0000147
CCCGGGT150.002213659270.0000131
TTAGGTG207.8619865E-570.032
ATGTATA207.8619865E-570.028
ACGCTTC207.8619865E-570.015
CTTAGGT207.8619865E-570.031
GACGACT509.531504E-1055.99999623
CTCGGTG700.055.044
TAGATCT1100.054.09090839
AGATCTC1200.052.50000440
GAAATGT200.00691717152.530