FastQCFastQC Report
Wed 25 May 2016
SRR1779848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55184
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1499827.178167584807188No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG18953.433966367062917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11532.0893737315163814No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4960.8988112496375761RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4090.7411568570600174No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT2480.44940562481878804No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2080.37692084662220937No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1620.2935633516961438No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1260.22832705131922296No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1240.22470281240939402No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT1110.20114525949550596No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1050.19027254276601915No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG790.14315743693824298No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG790.14315743693824298No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT740.13409683966367061No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC630.11416352565961148No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG560.1014786894752102No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC560.1014786894752102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATA150.002205813870.062611
AGACTCG150.002213773669.9990960
CTCCGGA150.002213773669.9990934
CGTGCTA150.002213773669.9990965
CGTGCGT150.002213773669.9990940
GCGGTAC150.002213773669.999093
TTCGTGG150.002213773669.9990946
ACGGGAG150.002213773669.9990955
GCACCAA150.002213773669.9990913
AGCGGTA150.002213773669.999092
GAGACTC150.002213773669.9990959
CCTCAGT150.002213773669.999099
ACGGACG150.002213773669.9990934
CTTGACA150.002213773669.9990957
CGGTACG150.002213773669.999094
CCAAAAT150.002213773669.9990916
GTGCGTT150.002213773669.9990941
AGTCGCG150.002213773669.9990935
TCTTTAT900.062.277881
TTTATAC1050.053.332653