FastQCFastQC Report
Wed 25 May 2016
SRR1779847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737777
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT608.367351E-1152.5042754
TAGACCG200.006952545652.497155
CTCGGTG1450.048.27324344
TCGGTGG1750.039.99511745
TCTCGGT1850.037.83578543
CGACGAA1850.037.83065822
TGGTCGC904.5019988E-935.0028549
GATCTCG2100.031.66494841
CCGTGCG802.4680667E-630.6233399
GACGACA1151.4570105E-930.43725823
ATCTCGG2300.030.43313442
GACGAAT1052.0228981E-830.0024423
TTCGGTA604.9802096E-429.16903926
CGGTAAG755.5822988E-528.00227728
TACGATT755.584878E-528.0003838
AGATCTC2500.027.9984840
ACTCGGT500.0072106327.9984843
GGGTCTA500.007215403527.9946881
CGTTCGG657.952836E-426.92526624
ACGTGTA657.952836E-426.92526634
Produced by FastQC (version 0.11.5)