FastQCFastQC Report
Wed 25 May 2016
SRR1779847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737777
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT200452.716945635334254No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG26230.3555274832368046No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC16110.21835866393232645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG200.006956380352.48983430
TCGTATG29750.050.81368340
TATGCCG29750.050.6960643
CGTATGC29850.050.5262341
CCGTCTT29900.050.46567547
GCCGTCT29950.050.38142846
TCTCGTA28600.050.2874638
CTCGTAT29750.050.10793739
CGTCTTC30300.050.03055248
ATCTCGT29000.049.3525137
ATGCCGT30800.048.85417644
TGCCGTC30900.048.8225545
GTATGCC30950.048.73046542
GATCTCG29700.048.1893236
GTCTTCT31900.047.5211849
AGATCTC30100.047.2001635
CTGCTTG32550.046.57853754
CTTGAAA31400.046.50025657
TCTTCTG32850.046.36633750
ACAGATC30750.046.20243533
Produced by FastQC (version 0.11.5)