##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779847_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	737777
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.539019242941976	34.0	34.0	34.0	34.0	34.0
2	33.602454400177834	34.0	34.0	34.0	34.0	34.0
3	33.73862020637672	34.0	34.0	34.0	34.0	34.0
4	33.57173373526147	34.0	34.0	34.0	34.0	34.0
5	33.58768977617898	34.0	34.0	34.0	34.0	34.0
6	37.257324367661234	38.0	38.0	38.0	36.0	38.0
7	37.34178484826716	38.0	38.0	38.0	37.0	38.0
8	37.238351154888264	38.0	38.0	38.0	36.0	38.0
9	37.14088674491072	38.0	38.0	38.0	36.0	38.0
10-11	37.09626960450109	38.0	37.5	38.0	35.5	38.0
12-13	37.25262985970015	38.0	38.0	38.0	36.5	38.0
14-15	37.27726874109656	38.0	38.0	38.0	36.0	38.0
16-17	37.233829463374434	38.0	38.0	38.0	36.0	38.0
18-19	37.124072043449445	38.0	38.0	38.0	36.0	38.0
20-21	37.106787010167025	38.0	38.0	38.0	36.0	38.0
22-23	37.0674905831979	38.0	38.0	38.0	36.0	38.0
24-25	37.2828815482185	38.0	38.0	38.0	36.5	38.0
26-27	37.20887137983429	38.0	38.0	38.0	36.0	38.0
28-29	36.752696275432825	38.0	37.0	38.0	34.5	38.0
30-31	37.05892905308786	38.0	38.0	38.0	36.0	38.0
32-33	37.120826482799	38.0	38.0	38.0	36.0	38.0
34-35	37.20961957339413	38.0	38.0	38.0	36.0	38.0
36-37	37.18121261573619	38.0	38.0	38.0	36.0	38.0
38-39	36.99924977330548	38.0	38.0	38.0	36.0	38.0
40-41	37.01941575842022	38.0	38.0	38.0	36.0	38.0
42-43	37.2039728806943	38.0	38.0	38.0	36.0	38.0
44-45	36.9796774635154	38.0	38.0	38.0	35.5	38.0
46-47	37.10576502113783	38.0	38.0	38.0	36.0	38.0
48-49	36.780243217123875	38.0	37.0	38.0	35.0	38.0
50-51	36.98061338317676	38.0	37.5	38.0	35.5	38.0
52-53	36.91410209317992	38.0	38.0	38.0	35.5	38.0
54-55	36.90414447726074	38.0	37.5	38.0	35.5	38.0
56-57	36.78316957563057	38.0	37.0	38.0	35.0	38.0
58-59	36.91452227434577	38.0	37.5	38.0	35.5	38.0
60-61	36.63142589156344	38.0	37.0	38.0	34.0	38.0
62-63	36.433101736703634	38.0	37.0	38.0	34.0	38.0
64-65	36.734240834290034	38.0	37.0	38.0	34.5	38.0
66-67	36.56816693933261	38.0	37.0	38.0	34.5	38.0
68-69	36.72185701099384	38.0	37.0	38.0	34.5	38.0
70-71	36.52527457483765	38.0	37.0	38.0	34.0	38.0
72-73	36.191776105788065	38.0	37.0	38.0	34.0	38.0
74-75	36.15591364328246	38.0	37.0	38.0	34.0	38.0
76	35.861172142801955	38.0	37.0	38.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	10.0
22	35.0
23	72.0
24	153.0
25	327.0
26	549.0
27	1021.0
28	1811.0
29	2998.0
30	5043.0
31	8250.0
32	12928.0
33	20613.0
34	33930.0
35	60608.0
36	109004.0
37	480424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99984811458669	18.332840609819932	10.444563180263575	27.222748095329795
2	21.980354497361667	21.456212378537145	32.74268512030058	23.820748003800606
3	19.55943327048688	22.343065723111454	31.869114922259705	26.22838608414196
4	14.039743716597291	20.044674745892053	36.58381868776067	29.331762849749992
5	13.71905060743287	32.88486900513299	37.43705753906668	15.95902284836746
6	27.702815349353532	39.28151731485259	19.054673702216252	13.960993633577626
7	26.659004007986155	30.183510735628786	22.3227343763766	20.834750880008457
8	24.120025427737648	36.54302045197939	20.42961491073861	18.907339209544347
9	23.76246480982736	17.928452635416935	21.126031307563125	37.18305124719258
10-11	24.297043686642443	26.185961340621894	27.601633013769742	21.91536195896592
12-13	24.345635605338742	23.20504705351346	27.318417353753233	25.130899987394567
14-15	22.39816367276291	23.48162114026325	23.800552199377318	30.319662987596523
16-17	24.229204759703812	28.096226908672943	24.42899412695164	23.245574204671602
18-19	23.484264215338783	26.60600696416397	26.179590851978308	23.730137968518942
20-21	21.99370541505089	26.288634641632907	25.78143531175409	25.936224631562112
22-23	22.16665740460871	28.001686146355876	26.00386024503339	23.827796204002023
24-25	23.840156579539478	27.61625542850637	25.780661234094005	22.762926757860154
26-27	23.611131818964267	28.134314298222908	25.889191449448816	22.365362433364012
28-29	23.326967362766798	26.249666227057773	26.724335402160815	23.699031008014618
30-31	22.35485790421767	25.962587611161638	29.51230520875549	22.1702492758652
32-33	21.22429948344825	29.83889440847302	27.024968249213515	21.911837858865212
34-35	21.95649905052611	27.73947954463205	26.52474934838034	23.779272056461505
36-37	23.920507145112953	27.8617387096643	25.885802891659676	22.33195125356307
38-39	21.70432257985814	26.378702507668304	28.215978540941233	23.70099637153232
40-41	21.77602446267639	25.093896936337128	28.65018833604192	24.479890264944558
42-43	22.99069702192201	25.085510545566432	29.00703399209379	22.91675844041777
44-45	21.07926921007296	26.394222102342578	29.62541526775706	22.9010934198274
46-47	22.499481550658263	24.704619417520473	28.428644427787802	24.367254604033466
48-49	21.82847933725231	24.736675174205754	28.536603878949872	24.89824160959206
50-51	20.54712997387034	25.09680852145445	29.6794965211291	24.676564983546108
52-53	19.40279889714531	25.392910626602877	31.654261698171148	23.550028778080662
54-55	19.156208723104758	25.262409173878964	30.084838852288726	25.49654325072756
56-57	20.53902602096378	25.198171331236686	28.426956996990643	25.835845650808892
58-59	18.964468739555162	24.791882907991873	31.447833270395947	24.795815082057015
60-61	20.761154891230657	26.545915793926707	27.94417537598776	24.74875393885487
62-63	18.890959540048797	28.938257611450783	27.056096719369975	25.114686129130448
64-65	19.1495317917312	29.39324410884529	25.87044712788203	25.586776971541482
66-67	19.188180123890938	29.21714947793468	25.158117893677794	26.43655250449659
68-69	19.02686626646397	29.454277056212508	25.344054807481808	26.17480186984172
70-71	19.879106898484633	28.71231495106595	25.071965827839165	26.336612322610247
72-73	20.217486500300293	28.108726981368175	25.177228043584066	26.496558474747463
74-75	20.39677472267522	28.044133376170745	24.99000234521258	26.569089555941456
76	20.334916230274224	28.26724975836308	24.93909936191585	26.458734649446846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.5
10	1.5
11	4.5
12	7.0
13	12.5
14	23.5
15	63.5
16	145.0
17	192.0
18	252.0
19	395.5
20	643.0
21	807.0
22	1034.5
23	1554.0
24	2288.0
25	2730.0
26	3362.0
27	4435.5
28	5693.0
29	6509.0
30	7505.5
31	9355.0
32	11815.5
33	13423.0
34	15133.5
35	18188.5
36	21730.5
37	23928.0
38	26300.5
39	34596.5
40	43619.5
41	47066.5
42	47414.0
43	46420.5
44	45403.0
45	44782.0
46	44185.0
47	43514.0
48	41402.5
49	38069.5
50	36177.0
51	34405.5
52	29673.5
53	25381.0
54	24049.0
55	22369.0
56	18922.5
57	15352.0
58	13548.0
59	12053.5
60	9789.0
61	8168.0
62	7317.0
63	6809.0
64	5701.0
65	4758.0
66	4227.0
67	4039.0
68	3747.0
69	3270.0
70	2997.5
71	2910.0
72	2681.5
73	2296.5
74	1986.0
75	1832.0
76	1642.0
77	1366.5
78	1171.5
79	1062.0
80	997.5
81	880.0
82	747.5
83	668.0
84	561.5
85	376.0
86	266.0
87	235.0
88	168.0
89	86.5
90	55.0
91	28.0
92	18.0
93	15.5
94	10.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05137053608339647
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.777115578284495E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.388557789142247E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014841883116443046
52-53	0.03205575668528566
54-55	0.05137053608339647
56-57	0.055572347741932863
58-59	0.03679973759008481
60-61	0.03429220482611955
62-63	0.029887079700234624
64-65	0.016875017789928393
66-67	0.037070822213216184
68-69	0.009149106030684069
70-71	0.013622002312351836
72-73	0.022771108343035903
74-75	0.014096400402831749
76	0.01355423115656899
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	737777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.710930200557637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.53562257154911	9.759004643425666
2	19.35154649478406	10.337956153900459
3	15.387292878734863	12.330267181782736
4	10.380368620879489	11.090772067534832
5	6.500039810958504	8.681105484567924
6	3.4588411868280025	5.543331930970603
7	1.9184604546254413	3.587070430722117
8	1.1008057692879993	2.3522836854258324
9	0.6856342321336788	1.6482535305872064
>10	4.265437127163608	22.78260738054667
>50	0.354781611423056	6.375428556703501
>100	0.05913026691112407	2.1296359951804322
>500	5.097436802683089E-4	0.07652039059468059
>1k	0.0010194873605366178	0.5764899177542306
>5k	0.0	0.0
>10k+	5.097436802683089E-4	2.729272650303154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	20045	2.716945635334254	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	2623	0.3555274832368046	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1611	0.21835866393232645	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006099404020456046	0.0
2	0.0	0.0	0.0	0.0062349463320217355	0.0
3	0.0	0.0	0.0	0.0062349463320217355	0.0
4	0.0	0.0	0.0	0.008945792563335534	0.0
5	0.0	0.0	0.0	0.010707842613689502	0.0
6	0.0	0.0	0.0	0.010707842613689502	0.0
7	0.0	0.0	0.0	0.011385554171517952	0.0
8	0.0	0.0	0.0	0.012198808040912092	0.0
9	0.0	0.0	0.0	0.0134186888450033	0.0
10	0.0	0.0	0.0	0.0147741119606602	0.0
11	0.0	0.0	0.0	0.01599399276475141	0.0
12	0.0	0.0	0.0	0.016671704322579858	0.0
13	0.0	0.0	0.0	0.01694278894571124	0.0
14	0.0	0.0	0.0	0.017620500503539686	0.0
15	0.0	0.0	0.0	0.018840381307630896	0.0
16	0.0	0.0	0.0	0.019653635177025036	0.0
17	0.0	0.0	0.0	0.020331346734853487	0.0
18	0.0	0.0	0.0	0.021280142915813316	0.0
19	0.0	0.0	0.0	0.022771108343035903	0.0
20	0.0	0.0	0.0	0.023719904523995732	0.0
21	0.0	0.0	0.0	0.026024123820612463	0.0
22	0.0	0.0	0.0	0.026566293066875222	0.0
23	0.0	0.0	0.0	0.02792171618253212	0.0
24	0.0	0.0	0.0	0.029006054675057638	0.0
25	0.0	0.0	0.0	0.031310273971674366	0.0
26	0.0	0.0	0.0	0.039849439600312835	0.0
27	0.0	0.0	0.0	0.09515070271911431	0.0
28	0.0	0.0	0.0	0.1409640040283175	0.0
29	0.0	0.0	0.0	0.17403632805034583	0.0
30	0.0	0.0	0.0	0.21280142915813316	0.0
31	0.0	0.0	0.0	0.2624099151911757	0.0
32	0.0	0.0	0.0	0.3076810472541161	0.0
33	0.0	0.0	0.0	0.3517322985129653	0.0
34	0.0	0.0	0.0	0.4199100812305073	0.0
35	0.0	0.0	0.0	0.534578876815081	0.0
36	0.0	0.0	0.0	0.7446694597419004	0.0
37	0.0	0.0	0.0	1.1083294816726463	0.0
38	0.0	0.0	0.0	1.6152577269283266	0.0
39	0.0	0.0	0.0	2.1830444700770015	0.0
40	0.0	0.0	0.0	2.8909819633846	0.0
41	0.0	0.0	0.0	3.7504557610226397	0.0
42	0.0	0.0	0.0	4.751842358869957	0.0
43	0.0	0.0	0.0	5.78982538084001	0.0
44	0.0	0.0	0.0	6.6453684514426445	0.0
45	0.0	0.0	0.0	7.486544037019316	0.0
46	0.0	0.0	0.0	8.354150373351297	0.0
47	0.0	0.0	0.0	9.20250970144095	0.0
48	0.0	0.0	0.0	9.998820781889378	0.0
49	0.0	0.0	0.0	10.797707166257554	0.0
50	0.0	0.0	0.0	11.640915886507711	0.0
51	0.0	0.0	0.0	12.567347586059203	0.0
52	0.0	0.0	0.0	13.607228200391175	0.0
53	0.0	0.0	0.0	14.677605834825428	0.0
54	0.0	0.0	0.0	15.62314900030768	0.0
55	0.0	0.0	0.0	16.419324538444542	0.0
56	0.0	0.0	0.0	17.175244010046395	0.0
57	0.0	0.0	0.0	17.916118285064456	0.0
58	0.0	0.0	0.0	18.633950367116352	0.0
59	0.0	0.0	0.0	19.28428237800853	0.0
60	0.0	0.0	0.0	19.97676804779764	0.0
61	0.0	0.0	0.0	20.672235648441195	0.0
62	0.0	0.0	0.0	21.373260483858942	0.0
63	0.0	0.0	0.0	22.132975140184637	0.0
64	0.0	0.0	0.0	22.86178614947335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACCG	20	0.0069563803	52.489834	30
TCGTATG	2975	0.0	50.813683	40
TATGCCG	2975	0.0	50.69606	43
CGTATGC	2985	0.0	50.52623	41
CCGTCTT	2990	0.0	50.465675	47
GCCGTCT	2995	0.0	50.381428	46
TCTCGTA	2860	0.0	50.28746	38
CTCGTAT	2975	0.0	50.107937	39
CGTCTTC	3030	0.0	50.030552	48
ATCTCGT	2900	0.0	49.35251	37
ATGCCGT	3080	0.0	48.854176	44
TGCCGTC	3090	0.0	48.82255	45
GTATGCC	3095	0.0	48.730465	42
GATCTCG	2970	0.0	48.18932	36
GTCTTCT	3190	0.0	47.52118	49
AGATCTC	3010	0.0	47.20016	35
CTGCTTG	3255	0.0	46.578537	54
CTTGAAA	3140	0.0	46.500256	57
TCTTCTG	3285	0.0	46.366337	50
ACAGATC	3075	0.0	46.202435	33
>>END_MODULE