FastQCFastQC Report
Wed 25 May 2016
SRR1779846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259756
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT200.00694419652.50346833
CGACTGG200.006949485752.49336230
ACGTGTA352.0821592E-550.00330434
ACGAATA305.8746175E-446.66076324
GTAGATC1300.045.7722538
TGGTCGC950.044.21344849
GCCGTAT404.583153E-543.75288854
CGACGAG855.638867E-1141.1712622
GTGTCGC350.001253567940.00264436
GTGTCGA350.001253567940.00264436
GTCGCTT459.1786664E-538.89145748
CGACGAA1350.038.8839722
TTACGCT459.189085E-538.8839713
GTGGTCG1100.038.18433848
TCGGTGG1151.8189894E-1236.51711745
TACCACG400.002417498334.9955755
AGCGATC400.002417498334.99557527
TTGCCGA400.002417498334.99557518
GACGATC400.002417498334.99557523
GACGACC708.674069E-734.99557523