##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779846_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259756
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96644158364003	34.0	23.0	34.0	12.0	34.0
2	29.851914873958638	34.0	31.0	34.0	12.0	34.0
3	29.540765179630114	34.0	31.0	34.0	11.0	34.0
4	29.61418793021143	34.0	27.0	34.0	12.0	34.0
5	30.418354147738647	34.0	32.0	34.0	12.0	34.0
6	32.859679853400884	37.0	34.0	38.0	12.0	38.0
7	33.15149986910793	38.0	34.0	38.0	12.0	38.0
8	32.39978287315789	37.0	31.0	38.0	11.0	38.0
9	33.33668519687707	38.0	34.0	38.0	21.0	38.0
10-11	31.841428109456565	37.0	28.5	38.0	11.5	38.0
12-13	32.71768505828547	37.0	32.0	38.0	11.0	38.0
14-15	31.510646529820292	37.0	28.5	38.0	11.0	38.0
16-17	32.144907143627094	37.0	30.5	38.0	11.5	38.0
18-19	33.02115831780594	37.5	34.0	38.0	11.5	38.0
20-21	31.660521797379076	37.0	28.0	38.0	11.0	38.0
22-23	32.606084171299216	37.0	32.5	38.0	11.0	38.0
24-25	32.91248517839819	37.5	33.0	38.0	11.0	38.0
26-27	31.805829316743406	37.0	29.0	38.0	11.0	38.0
28-29	33.05751743944317	37.5	33.0	38.0	16.0	38.0
30-31	33.50474483746285	38.0	34.0	38.0	21.0	38.0
32-33	33.594174917999965	38.0	34.0	38.0	21.0	38.0
34-35	33.52565292043302	38.0	34.0	38.0	21.0	38.0
36-37	33.01035779731748	38.0	33.0	38.0	11.0	38.0
38-39	32.95293467715857	38.0	33.0	38.0	11.0	38.0
40-41	32.92187475938958	37.5	33.0	38.0	11.0	38.0
42-43	33.34556468378016	38.0	34.0	38.0	16.0	38.0
44-45	33.392270053434764	38.0	34.0	38.0	21.0	38.0
46-47	33.28237461309845	38.0	34.0	38.0	15.5	38.0
48-49	32.98938426831334	37.5	33.0	38.0	15.0	38.0
50-51	32.34824219652289	37.5	30.5	38.0	11.0	38.0
52-53	32.900121652627845	37.0	33.5	38.0	11.0	38.0
54-55	33.0628493663284	37.5	33.5	38.0	15.0	38.0
56-57	33.10577234019618	38.0	34.0	38.0	11.0	38.0
58-59	33.39813709789187	38.0	34.0	38.0	16.0	38.0
60-61	33.24259689862794	38.0	34.0	38.0	11.0	38.0
62-63	33.26512188361386	38.0	34.0	38.0	11.0	38.0
64-65	33.28763146953295	38.0	34.0	38.0	14.5	38.0
66-67	33.11576055991007	38.0	34.0	38.0	11.0	38.0
68-69	32.49799042178044	37.0	31.5	38.0	11.0	38.0
70-71	32.517312400868505	37.0	31.5	38.0	11.0	38.0
72-73	32.721049754384886	37.0	32.0	38.0	11.0	38.0
74-75	32.90099554966969	37.0	32.5	38.0	11.0	38.0
76	31.765976531822172	37.0	29.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	59.0
13	629.0
14	2040.0
15	3272.0
16	4298.0
17	5003.0
18	5138.0
19	4888.0
20	3996.0
21	3257.0
22	2855.0
23	2694.0
24	2764.0
25	2969.0
26	3190.0
27	3797.0
28	4491.0
29	5233.0
30	6293.0
31	7359.0
32	9641.0
33	12222.0
34	16734.0
35	24092.0
36	41761.0
37	81078.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.72119667530308	17.921640943511722	9.659561198523212	32.69760118266199
2	22.512665732456615	20.495387979488445	30.470133509909296	26.52181277814564
3	18.925068140870664	21.91749179999692	30.12750427324104	29.029935785891375
4	15.411386070004157	20.302514667611142	30.525185173778468	33.76091408860623
5	15.345940036033817	31.68434992839434	34.27639785029027	18.69331218528157
6	30.93794393818648	35.79224559093587	16.644144924523292	16.625665546354366
7	27.840596281709228	29.973088783913333	18.64458329964619	23.541731634731253
8	24.535514918190568	35.59884504331088	19.13339749759384	20.732242540904718
9	24.537890834048813	17.181875233412775	19.327540204748722	38.952693727789686
10-11	24.044365907087226	25.701927630135245	24.286907745554362	25.966798717223167
12-13	24.403093672523447	22.41950137821648	25.4694790495696	27.707925899690476
14-15	24.86852945479184	24.363436174226383	22.209475118766218	28.55855925221556
16-17	25.663946780312873	25.559617098518405	22.72558406335237	26.050852057816353
18-19	22.78067269433852	25.471373050262468	25.13335117123811	26.614603084160905
20-21	24.624840234681777	24.95976993794176	24.542840203883646	25.87254962349282
22-23	25.642925890279116	24.8614051973051	23.512608277189607	25.98306063522618
24-25	23.048730016650786	27.30324642200599	23.368078614808613	26.279944946534616
26-27	24.08605834751342	27.278309477428643	23.906080368348515	24.729551806709424
28-29	22.91276451983622	26.7544982569074	25.106500936515243	25.226236286741138
30-31	23.44371982722114	26.169183150211357	25.03946041254418	25.34763661002333
32-33	22.41353423982507	26.91102419193397	25.042154945410307	25.63328662283066
34-35	22.474860249164575	27.210989112524448	25.049278531499763	25.264872106811215
36-37	24.319906664048332	25.563038347060342	24.864943956073578	25.252111032817755
38-39	24.267321700830085	24.700460474643094	26.061286248902714	24.970931575624107
40-41	22.785168303147778	26.213982818391983	25.380503513894855	25.620345364565388
42-43	21.418341969709804	25.658700154761817	26.388621541920433	26.53433633360795
44-45	21.368580926952937	24.162683570522457	27.798578212736796	26.67015728978781
46-47	21.746738172144433	24.821460883090086	27.130823455131342	26.300977489634143
48-49	21.61117516732145	25.002261750999498	27.241491485229325	26.145071596449725
50-51	23.30846317021432	25.116169840884854	25.976038406307627	25.599328582593195
52-53	19.71989604389258	26.29646741746078	26.986620463952253	26.99701607469439
54-55	20.414543480478876	25.511152863630326	26.570131077670595	27.5041725782202
56-57	20.52745670303945	25.84919027560662	26.29544351950962	27.32790950184431
58-59	20.188548117887812	25.941441120584933	26.601378784582717	27.268631976944533
60-61	19.842623683075598	25.430310824964575	26.62536196167827	28.101703530281558
62-63	20.67243553339558	25.634751807182525	26.112945298437783	27.579867360984107
64-65	20.973825315992645	25.357772023219322	25.350840882180233	28.317561778607804
66-67	20.53880156792681	26.96241134205602	24.219117001532503	28.27967008848467
68-69	20.753979711651365	27.6720371118939	23.758734191834613	27.815248984620123
70-71	20.79093761429039	28.006198148255084	24.197994263825528	27.004869973629
72-73	21.162212315107837	27.168079585441163	24.74513563250252	26.924572466948483
74-75	21.09552016417017	27.37895626428163	24.110375947376223	27.415147624171976
76	21.401650041770772	27.16024192399644	24.043410804963212	27.39469722926957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	4.0
12	8.0
13	6.5
14	7.5
15	11.5
16	26.5
17	40.0
18	46.0
19	75.5
20	114.5
21	130.0
22	182.5
23	289.0
24	477.5
25	612.0
26	780.5
27	1099.0
28	1413.5
29	1578.0
30	1927.0
31	2584.0
32	3412.5
33	3933.0
34	4313.0
35	5225.0
36	6214.0
37	6671.0
38	7415.5
39	8930.5
40	10334.0
41	11393.0
42	11819.0
43	12374.5
44	13279.0
45	13905.0
46	14182.0
47	14235.5
48	14075.5
49	13640.0
50	13418.0
51	13068.5
52	12066.0
53	10980.0
54	10547.0
55	10202.0
56	9241.5
57	7950.0
58	7274.0
59	6816.0
60	5862.0
61	5108.0
62	4850.0
63	4500.0
64	3875.5
65	3339.5
66	2804.5
67	2531.0
68	2424.5
69	2141.5
70	1855.5
71	1746.0
72	1585.5
73	1345.5
74	1230.0
75	1194.0
76	1119.0
77	909.0
78	751.5
79	729.0
80	655.5
81	526.5
82	412.5
83	354.0
84	314.5
85	255.0
86	192.5
87	150.0
88	131.0
89	83.0
90	54.0
91	44.5
92	35.0
93	31.0
94	22.0
95	16.5
96	16.0
97	12.5
98	8.5
99	15.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011549300112413188
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0026948366928964103
7	0.005004696715379048
8	0.0023098600224826377
9	0.008854463419516777
10-11	0.0026948366928964103
12-13	0.0
14-15	7.69953340827546E-4
16-17	9.624416760344324E-4
18-19	0.003657278368930843
20-21	0.0
22-23	0.0023098600224826377
24-25	0.00327230169851707
26-27	7.69953340827546E-4
28-29	0.006352115061827254
30-31	7.69953340827546E-4
32-33	0.0
34-35	0.003079813363310184
36-37	0.018863856850274873
38-39	0.00923944008993055
40-41	5.774650056206594E-4
42-43	7.69953340827546E-4
44-45	0.004812208380172161
46-47	0.0034647900337239565
48-49	5.774650056206594E-4
50-51	0.0026948366928964103
52-53	0.01193427678282696
54-55	0.009431928425137437
56-57	0.014629113475723371
58-59	0.013281695129275167
60-61	0.023098600224826377
62-63	0.010971835106792529
64-65	0.022521135219205717
66-67	0.019248833520688645
68-69	3.84976670413773E-4
70-71	3.84976670413773E-4
72-73	0.0038497667041377296
74-75	0.00981690509555121
76	0.0019248833520688648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	259756.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.63025342695086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77474074688726	51.155204327535486
2	10.782003467714926	14.36815247008217
3	6.049294328439318	12.091980424743848
4	3.184139842938711	8.486401787274312
5	1.5293994694475121	5.095213712016595
6	0.7482757626315713	2.991468221843191
7	0.3606075390699865	1.6819160201131598
8	0.18662036633048745	0.9947649842584628
9	0.11765744952007393	0.7055591111182082
>10	0.267261027020151	2.429338941014521
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015399066816550917	0.0
2	0.0	0.0015399066816550917	0.0	0.0015399066816550917	0.0
3	0.0	0.0015399066816550917	0.0	0.0015399066816550917	0.0
4	0.0	0.0015399066816550917	0.0	0.0026948366928964103	0.0
5	0.0	0.0015399066816550917	0.0	0.0026948366928964103	0.0
6	0.0	0.0015399066816550917	0.0	0.0026948366928964103	0.0
7	0.0	0.0015399066816550917	0.0	0.0030798133633101834	0.0
8	0.0	0.0015399066816550917	0.0	0.0030798133633101834	0.0
9	0.0	0.0015399066816550917	0.0	0.008084510078689232	0.0
10	0.0	0.0015399066816550917	0.0	0.008084510078689232	0.0
11	0.0	0.0015399066816550917	0.0	0.008084510078689232	0.0
12	0.0	0.0015399066816550917	0.0	0.008469486749103004	0.0
13	0.0	0.0015399066816550917	0.0	0.009624416760344322	0.0
14	0.0	0.0015399066816550917	0.0	0.012704230123654507	0.0
15	0.0	0.0015399066816550917	0.0	0.013859160134895826	0.0
16	0.0	0.0015399066816550917	0.0	0.014244136805309598	0.0
17	0.0	0.0015399066816550917	0.0	0.015014090146137145	0.0
18	0.0	0.0015399066816550917	0.0	0.01578404348696469	0.0
19	0.0	0.0015399066816550917	0.0	0.020403763531929964	0.0
20	0.0	0.0015399066816550917	0.0	0.021558693543171283	0.0
21	0.0	0.0015399066816550917	0.0	0.02386855356565392	0.0
22	0.0	0.0015399066816550917	0.0	0.033877946996412014	0.0
23	0.0	0.0015399066816550917	0.0	0.034647900337239565	0.0
24	0.0	0.0015399066816550917	0.0	0.045042270438411434	0.0
25	0.0	0.0015399066816550917	0.0	0.04735213046089407	0.0
26	0.0	0.0015399066816550917	0.0	0.05081692049461803	0.0
27	0.0	0.0015399066816550917	0.0	0.08854463419516777	0.0
28	0.0	0.0015399066816550917	0.0	0.1266573245661313	0.0
29	0.0	0.0015399066816550917	0.0	0.1620751782441984	0.0
30	0.0	0.0015399066816550917	0.0	0.2063474953417823	0.0
31	0.0	0.0015399066816550917	0.0	0.24523013905357335	0.0
32	0.0	0.0015399066816550917	0.0	0.3141409630576387	0.0
33	0.0	0.0015399066816550917	0.0	0.36226304685936034	0.0
34	0.0	0.0015399066816550917	0.0	0.4477278676912179	0.0
35	0.0	0.0015399066816550917	0.0	0.5547513820662467	0.0
36	0.0	0.0015399066816550917	0.0	0.696807773448929	0.0
37	0.0	0.0015399066816550917	0.0	1.0556060302745653	0.0
38	0.0	0.0015399066816550917	0.0	1.5572306318237115	0.0
39	0.0	0.0015399066816550917	0.0	2.0815688569272703	0.0
40	0.0	0.0015399066816550917	0.0	2.687137159488135	0.0
41	0.0	0.0015399066816550917	0.0	3.4528557569411293	0.0
42	0.0	0.0015399066816550917	0.0	4.359475815765564	0.0
43	0.0	0.0015399066816550917	0.0	5.231062997582346	0.0
44	0.0	0.0015399066816550917	0.0	6.06607739570982	0.0
45	0.0	0.0015399066816550917	0.0	6.854124640046813	0.0
46	0.0	0.0015399066816550917	0.0	7.631777514282635	0.0
47	0.0	0.0015399066816550917	0.0	8.317420964289564	0.0
48	0.0	0.0015399066816550917	0.0	9.008069111011872	0.0
49	0.0	0.0015399066816550917	0.0	9.64443554720584	0.0
50	0.0	0.0015399066816550917	0.0	10.314294953725804	0.0
51	0.0	0.0015399066816550917	0.0	11.008022913811423	0.0
52	0.0	0.0015399066816550917	0.0	11.901938742512204	0.0
53	0.0	0.0015399066816550917	0.0	12.764286484239054	0.0
54	0.0	0.0015399066816550917	0.0	13.575047352130461	0.0
55	0.0	0.0015399066816550917	0.0	14.257995965444493	0.0
56	0.0	0.0015399066816550917	0.0	14.905141748410045	0.0
57	0.0	0.0015399066816550917	0.0	15.49839079751767	0.0
58	0.0	0.0015399066816550917	0.0	16.108193843453087	0.0
59	0.0	0.0015399066816550917	0.0	16.63715178860161	0.0
60	0.0	0.0015399066816550917	0.0	17.19960270407613	0.0
61	0.0	0.0015399066816550917	0.0	17.72163106915721	0.0
62	0.0	0.0015399066816550917	0.0	18.27599747455304	0.0
63	0.0	0.0015399066816550917	0.0	18.861931966922803	0.0
64	0.0	0.0015399066816550917	0.0	19.494833613083046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTGT	20	0.006944196	52.503468	33
CGACTGG	20	0.0069494857	52.493362	30
ACGTGTA	35	2.0821592E-5	50.003304	34
ACGAATA	30	5.8746175E-4	46.660763	24
GTAGATC	130	0.0	45.77225	38
TGGTCGC	95	0.0	44.213448	49
GCCGTAT	40	4.583153E-5	43.752888	54
CGACGAG	85	5.638867E-11	41.17126	22
GTGTCGC	35	0.0012535679	40.002644	36
GTGTCGA	35	0.0012535679	40.002644	36
GTCGCTT	45	9.1786664E-5	38.891457	48
CGACGAA	135	0.0	38.88397	22
TTACGCT	45	9.189085E-5	38.88397	13
GTGGTCG	110	0.0	38.184338	48
TCGGTGG	115	1.8189894E-12	36.517117	45
TACCACG	40	0.0024174983	34.995575	5
AGCGATC	40	0.0024174983	34.995575	27
TTGCCGA	40	0.0024174983	34.995575	18
GACGATC	40	0.0024174983	34.995575	23
GACGACC	70	8.674069E-7	34.995575	23
>>END_MODULE