FastQCFastQC Report
Wed 25 May 2016
SRR1779846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259756
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT222148.551871756571552No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG28301.0894839772709775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16640.6406011795685181No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT6160.23714562897488414RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5100.1963381019110242No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT4080.15707048152881936No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT3240.12473244121406243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT150.002225119469.98286421
TACGGTC207.92669E-569.98286410
TCTCGTA31750.053.5616938
ATCTCGT32100.053.08669337
CCGTCTT33200.052.8338547
GATCTCG32150.052.78645736
CGTATGC33450.052.7225241
TATGCCG33450.052.7225243
CTCGTAT33350.052.56584539
TCGTATG33500.052.5393840
CGTAGTG200.006926305652.53771654
TCGTTCT200.00693158652.52759662
CGTCTTC33200.052.5174848
TCGCCAA200.006952737552.48715211
ATTACGC200.006952737552.4871528
TCGATAC200.006952737552.48715228
GAAGTCG200.006952737552.48715227
TTACGCT200.006952737552.4871529
GCCGTCT33500.052.4652246
GTATGCC33650.052.4091642