##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779846_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259756
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.526270807989036	34.0	34.0	34.0	34.0	34.0
2	33.61020727143935	34.0	34.0	34.0	34.0	34.0
3	33.74375182863918	34.0	34.0	34.0	34.0	34.0
4	33.57473937079413	34.0	34.0	34.0	34.0	34.0
5	33.603912902878086	34.0	34.0	34.0	34.0	34.0
6	37.263936155468976	38.0	38.0	38.0	36.0	38.0
7	37.33890651226535	38.0	38.0	38.0	37.0	38.0
8	37.23198694159134	38.0	38.0	38.0	36.0	38.0
9	37.15042578419748	38.0	38.0	38.0	36.0	38.0
10-11	37.102140085310836	38.0	38.0	38.0	35.5	38.0
12-13	37.259052726404775	38.0	38.0	38.0	36.5	38.0
14-15	37.28181254715964	38.0	38.0	38.0	36.0	38.0
16-17	37.208387871694974	38.0	38.0	38.0	36.0	38.0
18-19	37.09345501162629	38.0	38.0	38.0	36.0	38.0
20-21	37.09342613837602	38.0	38.0	38.0	36.0	38.0
22-23	37.083768998598686	38.0	38.0	38.0	36.0	38.0
24-25	37.264194089838156	38.0	38.0	38.0	36.0	38.0
26-27	37.178885184557814	38.0	38.0	38.0	36.0	38.0
28-29	36.67109710651535	38.0	37.0	38.0	34.0	38.0
30-31	36.93540476447127	38.0	37.0	38.0	35.5	38.0
32-33	36.97701496789294	38.0	38.0	38.0	36.0	38.0
34-35	37.03846301914104	38.0	38.0	38.0	36.0	38.0
36-37	37.06714378108687	38.0	38.0	38.0	36.0	38.0
38-39	36.49542840203884	38.0	37.0	38.0	34.0	38.0
40-41	36.80762523291089	38.0	37.0	38.0	35.0	38.0
42-43	37.16043325274488	38.0	38.0	38.0	36.0	38.0
44-45	36.92324720121961	38.0	38.0	38.0	35.5	38.0
46-47	37.081056837955614	38.0	38.0	38.0	36.0	38.0
48-49	36.73515914935555	38.0	37.0	38.0	34.5	38.0
50-51	36.90174817906035	38.0	37.5	38.0	35.5	38.0
52-53	36.83149763624324	38.0	37.5	38.0	35.0	38.0
54-55	36.8244313894578	38.0	37.5	38.0	35.0	38.0
56-57	36.70835322379463	38.0	37.0	38.0	34.5	38.0
58-59	36.79575640216203	38.0	37.0	38.0	35.0	38.0
60-61	36.49521473998676	38.0	37.0	38.0	34.0	38.0
62-63	36.298158656585414	38.0	37.0	38.0	34.0	38.0
64-65	36.645602026517196	38.0	37.0	38.0	34.0	38.0
66-67	36.43017293152035	38.0	37.0	38.0	34.0	38.0
68-69	36.51848657971327	38.0	37.0	38.0	34.0	38.0
70-71	35.82263547329032	38.0	37.0	38.0	31.0	38.0
72-73	34.61732933984201	38.0	36.5	38.0	23.0	38.0
74-75	34.44438434530868	38.0	36.5	38.0	23.0	38.0
76	34.14939404672077	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	3.0
22	12.0
23	40.0
24	63.0
25	150.0
26	242.0
27	507.0
28	838.0
29	1345.0
30	2227.0
31	3578.0
32	5657.0
33	8798.0
34	14907.0
35	28072.0
36	40509.0
37	152807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.211940298507464	16.162542128069333	10.653442465093885	33.97207510832932
2	19.52678667672739	17.88409122407182	37.78353531775974	24.805586781441043
3	18.587443600917787	18.66520888834137	36.17356288208934	26.573784628651502
4	13.332512049769784	23.90551132601364	32.3903971419332	30.371579482283373
5	13.282080105945578	30.968293321424724	40.30744236899244	15.442184203637261
6	28.542940297817953	40.47336731394078	16.91471996796994	14.068972420271331
7	26.826714301113352	27.367606523044703	18.747978872480328	27.057700303361614
8	23.883567655800057	40.111874220422244	16.778053249973052	19.226504873804647
9	22.635088313648193	16.235621121360044	17.79516161320624	43.33412895178552
10-11	23.342290457198295	27.48021219914073	27.37338117310091	21.804116170560064
12-13	23.589445479603935	20.190486456520734	28.23476647315173	27.985301590723605
14-15	21.164669921002787	20.554674386732163	21.29498452393785	36.9856711683272
16-17	26.823826976085247	28.044972974637737	21.53290010625356	23.598299943023456
18-19	26.76261568548946	24.073553642649255	22.77560479834922	26.388225873512066
20-21	21.69247293614007	22.731524969586843	22.293998983661588	33.2820031106115
22-23	21.407205223363466	28.248818121621827	22.29265156531514	28.051325089699564
24-25	26.89334442083218	28.149350149373248	22.32206104284086	22.63524438695371
26-27	26.779554658987664	28.859391120898074	22.805248001971083	21.555806218143182
28-29	21.245130045119264	28.802992038682458	22.89437010117187	27.057507815026412
30-31	20.56352885015168	23.86547375229061	28.59664454334067	26.97435285421703
32-33	20.682486641309534	28.307334575524724	23.450661389919773	27.55951739324597
34-35	21.058993824974205	23.04316358428679	28.746400468131633	27.15144212260737
36-37	26.4507845824543	27.476169944101386	23.405426631146153	22.667618842298157
38-39	21.44628035541046	23.822933830209887	28.586057684904294	26.144728129475354
40-41	20.719636890004466	22.00160920248233	29.617602673278	27.661151234235206
42-43	26.02254812698865	21.825716876062298	29.19861177145431	22.953123225494746
44-45	20.675172084571674	26.869831688199696	30.49169220345247	21.96330402377616
46-47	25.245615115723986	21.759458876792067	26.068695237068635	26.92623077041531
48-49	24.696060918708326	21.434346078627637	25.2712160643065	28.598376938357532
50-51	20.163293122899155	22.017041626077617	30.383263321230412	27.436401929792815
52-53	18.575293404064812	22.05204732975825	36.01682921428365	23.355830051893285
54-55	18.37173336517554	22.165064605654177	31.445591234662295	28.01761079450799
56-57	23.632574765419164	22.077561900066254	25.867640941096713	28.42222239341787
58-59	18.543588361031407	21.955747270311388	35.11621637235456	24.384447996302644
60-61	23.78123573847958	27.22730047350655	25.765380297583174	23.2260834904307
62-63	18.488509786177083	34.29311357356721	23.492023760241068	23.726352880014634
64-65	18.37290654714805	34.966318006534244	22.490600038119663	24.170175408198038
66-67	18.298213216306927	34.86702624428813	21.83673430089368	24.998026238511265
68-69	18.21968967774227	35.050051977053094	22.118931197782313	24.611327147422323
70-71	18.640364386810614	34.12487871740771	21.95291925274522	25.281837643036454
72-73	18.877507797758867	31.958103893103317	22.15391428241365	27.010474026724175
74-75	19.25738113939842	31.314204746723345	22.21387977637111	27.214534337507125
76	18.56486633219232	31.670972735078607	22.14730989445961	27.616851038269463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	3.5
14	2.5
15	14.5
16	38.5
17	51.0
18	58.0
19	80.5
20	138.5
21	181.0
22	209.5
23	328.0
24	526.0
25	634.0
26	820.0
27	1187.5
28	1568.5
29	1768.0
30	2092.5
31	2673.0
32	3355.0
33	3781.0
34	4254.0
35	5183.5
36	6101.5
37	6563.0
38	7150.0
39	8460.5
40	12906.0
41	18710.5
42	20793.0
43	20003.5
44	17865.5
45	14944.0
46	13371.0
47	13029.0
48	12383.0
49	11546.0
50	11013.0
51	10932.0
52	9916.0
53	8648.5
54	8316.0
55	8256.0
56	7705.0
57	6746.0
58	6278.0
59	5892.5
60	5021.0
61	4241.0
62	3947.0
63	3724.0
64	3272.0
65	2834.0
66	2511.0
67	2397.0
68	2267.0
69	1971.5
70	1671.5
71	1537.0
72	1420.5
73	1230.5
74	1105.5
75	1054.0
76	1017.5
77	888.0
78	694.0
79	593.0
80	541.5
81	456.5
82	352.5
83	282.0
84	253.0
85	199.5
86	142.0
87	109.0
88	94.0
89	67.0
90	31.5
91	10.5
92	13.0
93	7.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05043194382420425
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001539906681655092
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.924883352068865E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015014090146137145
52-53	0.0340704353316189
54-55	0.05485917553396264
56-57	0.055436640539583304
58-59	0.0427324104159288
60-61	0.03753522536534286
62-63	0.030220668627481174
64-65	0.018286391844654214
66-67	0.0383051787061704
68-69	0.010009393430758096
70-71	0.012319253453240735
72-73	0.025408460247309014
74-75	0.015399066816550918
76	0.017323950168619782
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	259756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.20582392707002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.85093573816603	11.499638121929811
2	18.994806252236028	11.854971588721723
3	15.958746098520832	14.940174625417699
4	11.305345489088195	14.11170483068726
5	7.103467844409628	11.083478341212523
6	4.109352446983062	7.694143734889666
7	2.219371075389531	4.848011210520642
8	1.1460787821216645	2.8611466145151603
9	0.624236667119012	1.7531837570643218
>10	1.6543505347956429	7.557861993563191
>50	0.01480403163128092	0.310291196353501
>100	0.012336693026067432	0.7699533408275459
>500	0.0024673386052134864	0.43348373088590836
>1k	0.0024673386052134864	1.7300851568394955
>5k	0.0	0.0
>10k+	0.0012336693026067432	8.551871756571552
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	22214	8.551871756571552	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	2830	1.0894839772709775	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1664	0.6406011795685181	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	616	0.23714562897488414	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	510	0.1963381019110242	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	408	0.15707048152881936	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	324	0.12473244121406243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0023098600224826377	0.0
2	0.0	0.0	0.0	0.0023098600224826377	0.0
3	0.0	0.0	0.0	0.0023098600224826377	0.0
4	0.0	0.0	0.0	0.003849766704137729	0.0
5	0.0	0.0	0.0	0.003849766704137729	0.0
6	0.0	0.0	0.0	0.003849766704137729	0.0
7	0.0	0.0	0.0	0.004234743374551502	0.0
8	0.0	0.0	0.0	0.004234743374551502	0.0
9	0.0	0.0	0.0	0.01193427678282696	0.0
10	0.0	0.0	0.0	0.01193427678282696	0.0
11	0.0	0.0	0.0	0.01193427678282696	0.0
12	0.0	0.0	0.0	0.012319253453240734	0.0
13	0.0	0.0	0.0	0.013089206794068279	0.0
14	0.0	0.0	0.0	0.014629113475723371	0.0
15	0.0	0.0	0.0	0.017323950168619782	0.0
16	0.0	0.0	0.0	0.018093903509447326	0.0
17	0.0	0.0	0.0	0.018863856850274873	0.0
18	0.0	0.0	0.0	0.02078874020234374	0.0
19	0.0	0.0	0.0	0.025793436917722786	0.0
20	0.0	0.0	0.0	0.026948366928964105	0.0
21	0.0	0.0	0.0	0.028488273610619196	0.0
22	0.0	0.0	0.0	0.032723016985170696	0.0
23	0.0	0.0	0.0	0.03657278368930843	0.0
24	0.0	0.0	0.0	0.043117387086342565	0.0
25	0.0	0.0	0.0	0.045042270438411434	0.0
26	0.0	0.0	0.0	0.05081692049461803	0.0
27	0.0	0.0	0.0	0.09200942422889173	0.0
28	0.0	0.0	0.0	0.13050709127026902	0.0
29	0.0	0.0	0.0	0.1709296416637152	0.0
30	0.0	0.0	0.0	0.2163568887725404	0.0
31	0.0	0.0	0.0	0.2598592525292967	0.0
32	0.0	0.0	0.0	0.3314649132262585	0.0
33	0.0	0.0	0.0	0.3869015537658418	0.0
34	0.0	0.0	0.0	0.47968093133556106	0.0
35	0.0	0.0	0.0	0.5963288624709343	0.0
36	0.0	0.0	0.0	0.7533993439997536	0.0
37	0.0	0.0	0.0	1.1603196846271115	0.0
38	0.0	0.0	0.0	1.693512373150187	0.0
39	0.0	0.0	0.0	2.243259058501055	0.0
40	0.0	0.0	0.0	2.8934846548299173	0.0
41	0.0	0.0	0.0	3.6957760359722203	0.0
42	0.0	0.0	0.0	4.6651472920741	0.0
43	0.0	0.0	0.0	5.585626511033431	0.0
44	0.0	0.0	0.0	6.466453132940144	0.0
45	0.0	0.0	0.0	7.308012134464652	0.0
46	0.0	0.0	0.0	8.140716672569642	0.0
47	0.0	0.0	0.0	8.87294229969664	0.0
48	0.0	0.0	0.0	9.60362802014198	0.0
49	0.0	0.0	0.0	10.288501516808081	0.0
50	0.0	0.0	0.0	10.993778777006114	0.0
51	0.0	0.0	0.0	11.70868045396449	0.0
52	0.0	0.0	0.0	12.623769999538029	0.0
53	0.0	0.0	0.0	13.50652150479681	0.0
54	0.0	0.0	0.0	14.339995996242628	0.0
55	0.0	0.0	0.0	15.026409399590385	0.0
56	0.0	0.0	0.0	15.674710112567178	0.0
57	0.0	0.0	0.0	16.27604367175349	0.0
58	0.0	0.0	0.0	16.890466437733874	0.0
59	0.0	0.0	0.0	17.439828146414328	0.0
60	0.0	0.0	0.0	18.00035417853678	0.0
61	0.0	0.0	0.0	18.538551563775236	0.0
62	0.0	0.0	0.0	19.109856942669275	0.0
63	0.0	0.0	0.0	19.720814918615932	0.0
64	0.0	0.0	0.0	20.383744745068448	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAT	15	0.0022251194	69.982864	21
TACGGTC	20	7.92669E-5	69.982864	10
TCTCGTA	3175	0.0	53.56169	38
ATCTCGT	3210	0.0	53.086693	37
CCGTCTT	3320	0.0	52.83385	47
GATCTCG	3215	0.0	52.786457	36
CGTATGC	3345	0.0	52.72252	41
TATGCCG	3345	0.0	52.72252	43
CTCGTAT	3335	0.0	52.565845	39
TCGTATG	3350	0.0	52.53938	40
CGTAGTG	20	0.0069263056	52.537716	54
TCGTTCT	20	0.006931586	52.527596	62
CGTCTTC	3320	0.0	52.51748	48
TCGCCAA	20	0.0069527375	52.487152	11
ATTACGC	20	0.0069527375	52.487152	8
TCGATAC	20	0.0069527375	52.487152	28
GAAGTCG	20	0.0069527375	52.487152	27
TTACGCT	20	0.0069527375	52.487152	9
GCCGTCT	3350	0.0	52.46522	46
GTATGCC	3365	0.0	52.40916	42
>>END_MODULE