FastQCFastQC Report
Wed 25 May 2016
SRR1779845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93226
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGT150.00221847870.050
TGCCGCT550.063.63636813
GTTATAC401.053013E-861.251
GGGTTTA352.9461808E-760.037
TCGGTGG1050.059.99999645
TCTCGGT1600.056.87543
TTGTCAT252.3796153E-455.9999962
CTCGGTC252.3796153E-455.99999644
TGTGTCG252.3796153E-455.99999635
TGGTAGC252.3796153E-455.99999649
ATCTCGG1950.055.6410242
CTCGGTG1300.053.84615744
GATCTCG1700.053.52940841
TGATCGG200.006932110552.531
TAGAGAG608.185452E-1152.529
TCGCACG200.006932110552.551
ACGTTGC200.006932110552.515
GTTTATC200.006932110552.539
AGGACCG200.006932110552.524
TTTCCTA200.006932110552.524
Produced by FastQC (version 0.11.5)