##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779845_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93226
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.867225881191942	24.0	12.0	34.0	12.0	34.0
2	25.168365048377062	31.0	12.0	34.0	12.0	34.0
3	24.946045094716066	31.0	11.0	34.0	11.0	34.0
4	25.688048398515434	31.0	12.0	34.0	12.0	34.0
5	25.299744706412373	32.0	11.0	34.0	11.0	34.0
6	27.353527985755047	33.0	12.0	38.0	11.0	38.0
7	27.82708686417952	34.0	12.0	38.0	12.0	38.0
8	27.073488082723703	32.0	11.0	38.0	11.0	38.0
9	28.008323858151158	34.0	12.0	38.0	11.0	38.0
10-11	26.876649218029307	31.0	11.5	37.5	11.0	38.0
12-13	27.25994357797181	32.0	11.0	38.0	11.0	38.0
14-15	26.383337266427823	29.0	11.0	37.0	11.0	38.0
16-17	26.641510951880377	29.5	11.0	37.5	11.0	38.0
18-19	26.92695707206144	31.0	11.0	38.0	11.0	38.0
20-21	26.422880955956494	29.0	11.0	37.5	11.0	38.0
22-23	26.887638641580676	32.5	11.0	38.0	11.0	38.0
24-25	27.149883079827518	32.0	11.0	38.0	11.0	38.0
26-27	26.32924827837728	29.5	11.0	38.0	11.0	38.0
28-29	26.85575912299144	31.0	11.0	38.0	11.0	38.0
30-31	26.993086692553582	32.5	11.0	38.0	11.0	38.0
32-33	27.148451075880118	34.0	11.0	38.0	11.0	38.0
34-35	27.196559972539852	34.0	11.0	38.0	11.0	38.0
36-37	26.858151159547766	32.0	11.0	38.0	11.0	38.0
38-39	26.78223886040375	32.0	11.0	38.0	11.0	38.0
40-41	26.780179349108618	31.0	11.0	38.0	11.0	38.0
42-43	27.035885911655548	32.5	11.0	38.0	11.0	38.0
44-45	27.12677793748525	33.0	11.0	38.0	11.0	38.0
46-47	27.000117992834618	32.5	11.0	38.0	11.0	38.0
48-49	26.6837738399159	31.0	11.0	38.0	11.0	38.0
50-51	26.28893227211293	29.0	11.0	38.0	11.0	38.0
52-53	26.788353034561172	31.0	11.0	38.0	11.0	38.0
54-55	26.794354579194646	31.0	11.0	38.0	11.0	38.0
56-57	26.84354150129792	31.5	11.0	38.0	11.0	38.0
58-59	26.810299701799927	31.5	11.0	38.0	11.0	38.0
60-61	26.925133546435546	31.5	11.0	38.0	11.0	38.0
62-63	26.781246647930836	31.0	11.0	38.0	11.0	38.0
64-65	26.775995966790383	31.0	11.0	38.0	11.0	38.0
66-67	26.882682942526763	31.0	11.0	38.0	11.0	38.0
68-69	26.609513440456524	31.0	11.0	38.0	11.0	38.0
70-71	26.606863964988307	31.0	11.0	38.0	11.0	38.0
72-73	26.501780619140582	31.0	11.0	38.0	11.0	38.0
74-75	26.670252933730936	31.0	11.0	38.0	11.0	38.0
76	25.831817304185527	27.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	58.0
13	758.0
14	2667.0
15	4488.0
16	5847.0
17	6750.0
18	6933.0
19	6146.0
20	4801.0
21	3374.0
22	2309.0
23	1647.0
24	1275.0
25	1064.0
26	991.0
27	932.0
28	996.0
29	1057.0
30	1235.0
31	1495.0
32	1888.0
33	2365.0
34	3130.0
35	4816.0
36	8531.0
37	17671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.32257095660009	20.815008688563278	15.530002359856693	35.33241799497994
2	13.823396906442408	30.4657498980971	31.391457318773732	24.319395876686762
3	12.815094501533908	30.479694505824558	32.1369575011263	24.568253491515243
4	10.428421255872825	31.503014180593397	24.53392830326304	33.53463626027074
5	11.220045909939287	33.830691008946005	34.86366464291078	20.085598438203935
6	18.942083865223502	41.46276053678892	19.733750978856694	19.86140461913088
7	18.01847223265144	34.77971701655206	17.327640767638194	29.8741699831583
8	16.386514057689627	41.29131222981453	20.13344346352295	22.18873024897289
9	18.80323542663434	20.668754961488126	17.884957840760368	42.64305177111717
10-11	17.488522268943623	32.59139277439286	23.48536857461598	26.43471638204754
12-13	17.37069057988115	25.384013043571535	25.482161628730182	31.763134747817134
14-15	25.325152452923287	28.042756541933265	19.41475240143523	27.217338603708214
16-17	26.257837179741593	26.366712970163743	19.173402127100417	28.202047722994244
18-19	18.782079818073573	26.680754522684484	23.631663350299544	30.90550230894239
20-21	25.443545791946455	25.392594340634588	20.66322699676056	28.5006328706584
22-23	26.135041057244152	25.137438521402867	19.376015704194756	29.351504717158228
24-25	18.631131802237647	31.61237033779218	19.33428445769821	30.422213402271968
26-27	23.773257030072568	30.993462019104417	20.262377379336975	24.970903571486037
28-29	19.564354523804415	30.468900856057846	23.283056920337273	26.68368769980047
30-31	21.187872416881646	28.160750009385843	23.47480034968973	27.17657722404278
32-33	18.46705032421387	30.13553158738757	23.121356281274974	28.276061807123586
34-35	18.705884876960376	31.818669412799554	22.57299779022119	26.90244792001888
36-37	23.164935845170152	25.99826202634854	23.52969574732867	27.30710638115264
38-39	23.59210646298094	24.234703455970305	25.69315188997538	26.480038191073373
40-41	20.03689977527608	29.557363596869955	23.423312291164972	26.982424336688997
42-43	16.529723467702144	28.86212000943943	25.1726986033939	29.435457919464525
44-45	16.13593576520186	25.271265440541512	27.97828803750248	30.61451075675415
46-47	18.652485692985138	27.45391451726228	25.326768465032956	28.566831324719626
48-49	19.720356981957824	27.62319524596143	24.588097740973545	28.068350031107205
50-51	25.118801154221597	27.059845746221427	20.617766001952308	27.20358709760467
52-53	14.970525604372616	30.32295784544582	24.171149957357336	30.535366592824232
54-55	17.581368405243623	27.1626724452359	23.31148490634856	31.94447424317192
56-57	18.941490731204944	28.654415550978374	21.55102128389976	30.85307243391692
58-59	17.971356541329186	29.15410609880384	22.627259561229415	30.247277798637555
60-61	15.644747712081712	27.0038730996599	23.87803491154098	33.47334427671741
62-63	17.451122375090513	28.249523962775232	22.65078981950814	31.648563842626114
64-65	17.959790153522654	27.022025297443435	22.652369355548167	32.365815193485744
66-67	16.23001823838644	33.24214140113722	19.483960948396096	31.04387941208025
68-69	16.93420791521633	35.47634499144547	18.268606765316356	29.32084032802184
70-71	16.08349602042349	35.28843884753181	19.81904189818291	28.809023233861797
72-73	17.40744117694383	32.952174589297876	21.51580324914588	28.124580984612415
74-75	16.628942287062863	33.037974683544306	19.692662518772796	30.640420510620036
76	18.209707696433362	33.22499329578976	19.03781174577635	29.527487262000534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	1.5
14	2.5
15	5.5
16	13.0
17	17.0
18	19.5
19	18.5
20	26.0
21	37.0
22	38.5
23	56.5
24	99.0
25	125.0
26	156.5
27	244.5
28	399.0
29	497.0
30	564.0
31	688.5
32	942.0
33	1138.0
34	1260.5
35	1652.0
36	2190.0
37	2459.0
38	2713.5
39	3261.5
40	3871.0
41	4544.0
42	4901.0
43	5161.5
44	5597.0
45	5889.0
46	6006.0
47	6133.5
48	6225.0
49	5975.0
50	5761.0
51	5391.5
52	4697.5
53	4115.0
54	3857.0
55	3568.0
56	3059.5
57	2588.5
58	2337.0
59	2186.5
60	1873.0
61	1546.0
62	1382.0
63	1199.0
64	965.0
65	804.5
66	666.5
67	638.0
68	568.0
69	445.5
70	353.5
71	314.0
72	276.5
73	233.5
74	196.5
75	165.0
76	158.5
77	127.0
78	104.0
79	106.0
80	94.5
81	65.5
82	43.5
83	39.0
84	31.5
85	23.5
86	17.5
87	12.0
88	11.0
89	10.0
90	9.0
91	7.0
92	6.0
93	6.5
94	7.5
95	7.0
96	6.0
97	5.5
98	6.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.005363310664406925
7	0.005363310664406925
8	0.0032179863986441554
9	0.00858129706305108
10-11	0.00214532426576277
12-13	0.0
14-15	5.363310664406925E-4
16-17	5.363310664406925E-4
18-19	0.0016089931993220777
20-21	0.0
22-23	0.0026816553322034625
24-25	0.0032179863986441554
26-27	0.0016089931993220777
28-29	0.00858129706305108
30-31	5.363310664406925E-4
32-33	5.363310664406925E-4
34-35	0.00429064853152554
36-37	0.01501726986033939
38-39	0.011262952395254542
40-41	5.363310664406925E-4
42-43	0.0
44-45	0.005899641730847617
46-47	0.0026816553322034625
48-49	0.0
50-51	0.0032179863986441554
52-53	0.010190290262373157
54-55	0.00858129706305108
56-57	0.01072662132881385
58-59	0.011799283461695235
60-61	0.020380580524746315
62-63	0.009117628129491772
64-65	0.016089931993220775
66-67	0.01716259412610216
68-69	5.363310664406925E-4
70-71	0.0
72-73	0.0016089931993220777
74-75	0.006435972797288311
76	0.001072662132881385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	93226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.28260356552893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.92721073709275	71.29663398622702
2	3.8085831799978354	6.039087808122198
3	2.4136811343218967	5.740887735181173
4	1.553198398095032	4.92566451419132
5	0.951131074791644	3.7704073970780683
6	0.5371252300032471	2.555081200523459
7	0.3355341487173937	1.8621414626820845
8	0.18535555796081826	1.1756376976379979
9	0.09741313995021106	0.6950850621071375
>10	0.18941443879207706	1.8642867869478472
>50	0.0013529602770862648	0.07508634930169696
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03861583678372986	0.0
2	0.0	0.0	0.0	0.04397914744813679	0.0
3	0.0	0.0	0.0	0.04397914744813679	0.0
4	0.0	0.0	0.0	0.05363310664406925	0.0
5	0.0	0.0	0.0	0.06221440370712033	0.0
6	0.0	0.0	0.0	0.06865037650440864	0.0
7	0.0	0.0	0.0	0.06865037650440864	0.0
8	0.0	0.0	0.0	0.06865037650440864	0.0
9	0.0	0.0	0.0	0.06865037650440864	0.0
10	0.0	0.0	0.0	0.06865037650440864	0.0
11	0.0	0.0	0.0	0.06972303863729003	0.0
12	0.0	0.0	0.0	0.06972303863729003	0.0
13	0.0	0.0	0.0	0.07079570077017142	0.0
14	0.0	0.0	0.0	0.08044965996610387	0.0
15	0.0	0.0	0.0	0.08044965996610387	0.0
16	0.0	0.0	0.0	0.08044965996610387	0.0
17	0.0	0.0	0.0	0.0858129706305108	0.0
18	0.0	0.0	0.0	0.08688563276339219	0.0
19	0.0	0.0	0.0	0.08688563276339219	0.0
20	0.0	0.0	0.0	0.08903095702915495	0.0
21	0.0	0.0	0.0	0.09439426769356188	0.0
22	0.0	0.0	0.0	0.14051873940746143	0.0
23	0.0	0.0	0.0	0.14159140154034283	0.0
24	0.0	0.0	0.0	0.16304464419797052	0.0
25	0.0	0.0	0.0	0.1651899684637333	0.0
26	0.0	0.0	0.0	0.16626263059661467	0.0
27	0.0	0.0	0.0	0.17698925192542853	0.0
28	0.0	0.0	0.0	0.19736983245017484	0.0
29	0.0	0.0	0.0	0.21667775084203977	0.0
30	0.0	0.0	0.0	0.25422092549288827	0.0
31	0.0	0.0	0.0	0.2735288438847532	0.0
32	0.0	0.0	0.0	0.3078540321369575	0.0
33	0.0	0.0	0.0	0.3421792203891618	0.0
34	0.0	0.0	0.0	0.36899577371119646	0.0
35	0.0	0.0	0.0	0.4515907579430631	0.0
36	0.0	0.0	0.0	0.5620749576298457	0.0
37	0.0	0.0	0.0	0.7841160191362925	0.0
38	0.0	0.0	0.0	1.0201016883701972	0.0
39	0.0	0.0	0.0	1.3719348679552914	0.0
40	0.0	0.0	0.0	1.7731105056529295	0.0
41	0.0	0.0	0.0	2.185012764679381	0.0
42	0.0	0.0	0.0	2.6344581983566817	0.0
43	0.0	0.0	0.0	3.0635230515092355	0.0
44	0.0	0.0	0.0	3.5172591337180616	0.0
45	0.0	0.0	0.0	4.0160470255079055	0.0
46	0.0	0.0	0.0	4.497672323171647	0.0
47	0.0	0.0	0.0	4.993242228562847	0.0
48	0.0	0.0	0.0	5.458777594233369	0.0
49	0.0	0.0	0.0	5.755905005041512	0.0
50	0.0	0.0	0.0	6.134554737948641	0.0
51	0.0	0.0	0.0	6.610816724947976	0.0
52	0.0	0.0	0.0	6.9626499045330705	0.0
53	0.0	0.0	0.0	7.377770149958166	0.0
54	0.0	0.0	0.0	7.776800463390042	0.0
55	0.0	0.0	0.0	8.121125008044967	0.0
56	0.0	0.0	0.0	8.524445970008367	0.0
57	0.0	0.0	0.0	8.947074850363633	0.0
58	0.0	0.0	0.0	9.338596528865338	0.0
59	0.0	0.0	0.0	9.745135477227382	0.0
60	0.0	0.0	0.0	10.058352820028748	0.0
61	0.0	0.0	0.0	10.369424838564349	0.0
62	0.0	0.0	0.0	10.629009074721644	0.0
63	0.0	0.0	0.0	11.00551348336301	0.0
64	0.0	0.0	0.0	11.325166798961662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCGT	15	0.002218478	70.0	50
TGCCGCT	55	0.0	63.636368	13
GTTATAC	40	1.053013E-8	61.25	1
GGGTTTA	35	2.9461808E-7	60.0	37
TCGGTGG	105	0.0	59.999996	45
TCTCGGT	160	0.0	56.875	43
TTGTCAT	25	2.3796153E-4	55.999996	2
CTCGGTC	25	2.3796153E-4	55.999996	44
TGTGTCG	25	2.3796153E-4	55.999996	35
TGGTAGC	25	2.3796153E-4	55.999996	49
ATCTCGG	195	0.0	55.64102	42
CTCGGTG	130	0.0	53.846157	44
GATCTCG	170	0.0	53.529408	41
TGATCGG	20	0.0069321105	52.5	31
TAGAGAG	60	8.185452E-11	52.5	29
TCGCACG	20	0.0069321105	52.5	51
ACGTTGC	20	0.0069321105	52.5	15
GTTTATC	20	0.0069321105	52.5	39
AGGACCG	20	0.0069321105	52.5	24
TTTCCTA	20	0.0069321105	52.5	24
>>END_MODULE