FastQCFastQC Report
Wed 25 May 2016
SRR1779845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93226
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3206134.39062064231008No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG41244.423658636002832No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC22672.4317250552421No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT8320.8924548945573124RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC7750.8313131529830734No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3430.3679231115783151No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT3370.36148713878102673No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3120.3346705854589921No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG2560.27460150601763456No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2470.2649475468217021No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2240.24027631776543024No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1670.1791345761911913No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT1650.17698925192542853No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1450.15553600926780084No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT1340.1437367258061056No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1250.13408276661017313No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT1240.13301010447729172No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1170.12550146954712205No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT1140.1222834831484779No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1110.11906549674983373No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG1090.11692017248407097No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1060.1137021860854268No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC150.002213825270.037031
TGTTTCG150.002218545469.99946654
GAGTCGG150.002218545469.99946648
TCGGGCC150.002218545469.99946651
CGTTAAT150.002218545469.99946632
CCACTCG150.002218545469.99946623
CGGATCG150.002218545469.99946651
AGCTGGC150.002218545469.99946617
GACATTA252.8206778E-669.99946626
GACATAC150.002218545469.99946626
GACGCCT150.002218545469.99946628
ATCGGAG150.002218545469.99946654
GCAGAAT150.002218545469.99946625
TGTGTTC150.002218545469.99946610
AGTCGGG150.002218545469.99946649
AACACGA150.002218545469.99946669
TCTTTAT1200.064.2006151
AGACATT352.9463263E-759.9995425
CGTCTGC308.3204595E-658.33288641
TCAGTTA308.3204595E-658.33288630
Produced by FastQC (version 0.11.5)