##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779845_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93226
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.546596443052366	34.0	34.0	34.0	34.0	34.0
2	33.625372750091174	34.0	34.0	34.0	34.0	34.0
3	33.76795100079377	34.0	34.0	34.0	34.0	34.0
4	33.57524724862163	34.0	34.0	34.0	34.0	34.0
5	33.63175508978182	34.0	34.0	34.0	34.0	34.0
6	37.36660373715487	38.0	38.0	38.0	37.0	38.0
7	37.40125072404694	38.0	38.0	38.0	37.0	38.0
8	37.31363568103319	38.0	38.0	38.0	37.0	38.0
9	37.22399330658829	38.0	38.0	38.0	36.0	38.0
10-11	37.15243601570377	38.0	38.0	38.0	36.5	38.0
12-13	37.31194087486324	38.0	38.0	38.0	36.5	38.0
14-15	37.33167785810825	38.0	38.0	38.0	37.0	38.0
16-17	37.153143972711476	38.0	38.0	38.0	36.0	38.0
18-19	36.95805354729367	38.0	38.0	38.0	35.0	38.0
20-21	37.06734172870229	38.0	38.0	38.0	35.5	38.0
22-23	37.14651492073027	38.0	38.0	38.0	36.0	38.0
24-25	37.246621114281425	38.0	38.0	38.0	36.0	38.0
26-27	37.07414240662476	38.0	38.0	38.0	36.0	38.0
28-29	36.49223392615794	38.0	37.0	38.0	34.0	38.0
30-31	36.589470748503636	38.0	37.0	38.0	35.0	38.0
32-33	36.57606783515328	38.0	37.0	38.0	34.0	38.0
34-35	36.646364748031665	38.0	37.0	38.0	34.0	38.0
36-37	36.59570291549568	38.0	37.0	38.0	34.5	38.0
38-39	35.08553407847596	37.5	35.5	38.0	29.0	38.0
40-41	36.32040954240233	38.0	37.0	38.0	34.0	38.0
42-43	37.0911494647416	38.0	37.5	38.0	36.0	38.0
44-45	36.87220303348851	38.0	37.5	38.0	35.0	38.0
46-47	37.02957865831421	38.0	38.0	38.0	36.0	38.0
48-49	36.596534228648665	38.0	37.0	38.0	34.0	38.0
50-51	36.67549288825006	38.0	37.0	38.0	34.0	38.0
52-53	36.60454701478128	38.0	37.0	38.0	34.0	38.0
54-55	36.624852508956735	38.0	37.0	38.0	34.0	38.0
56-57	36.46641494861949	38.0	37.0	38.0	34.0	38.0
58-59	36.48379207517216	38.0	37.0	38.0	34.0	38.0
60-61	36.05910368352176	38.0	37.0	38.0	33.0	38.0
62-63	36.00647351597194	38.0	37.0	38.0	33.0	38.0
64-65	36.50165189968464	38.0	37.0	38.0	34.0	38.0
66-67	36.11872760817798	38.0	37.0	38.0	32.5	38.0
68-69	35.97543067384635	38.0	37.0	38.0	31.5	38.0
70-71	33.12922360714822	37.5	30.0	38.0	21.0	38.0
72-73	28.156474588634072	32.5	21.5	38.0	11.0	38.0
74-75	27.346770214317893	32.0	19.0	38.0	11.0	38.0
76	27.351725913371805	32.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	7.0
23	10.0
24	22.0
25	60.0
26	115.0
27	264.0
28	480.0
29	828.0
30	1285.0
31	2007.0
32	3104.0
33	4929.0
34	8888.0
35	19906.0
36	20553.0
37	30766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.150455013736263	11.207932692307693	12.538633241758243	56.102979052197796
2	9.872782271040267	10.641881020316221	60.6601162765752	18.825220432068306
3	9.804131894535859	13.115439898740695	60.73627528800978	16.344152918713664
4	6.9626499045330705	47.71522965696265	29.003711410979772	16.31840902752451
5	6.506768498058483	25.108875206487465	60.396241391886385	7.9881149035676735
6	14.167721451097334	61.805719434492524	15.014051873940746	9.012507240469397
7	12.96419453800442	22.72327462295926	15.532147684122455	48.780383154913864
8	12.1586252762105	60.68371484349859	11.079527170531826	16.07813270975908
9	11.656619398022011	15.320833243944824	11.297277583506746	61.72526977452642
10-11	12.487396219938644	37.08192993370948	33.451504944972434	16.979168901379442
12-13	12.595735095359665	13.80945229871495	37.57320919056916	36.02160341535623
14-15	12.204213416857957	12.513140111127797	16.735674597215368	58.54697187479888
16-17	34.5397206787806	33.321176495827345	12.206895072190163	19.932207753201897
18-19	35.91004655353657	18.31624225001609	12.929869349752215	32.84384184669513
20-21	13.8153519404458	13.714521699954949	12.808122197670176	59.66200416192907
22-23	12.543174650848476	36.02321240855556	12.725527213438312	38.70808572715766
24-25	34.54722931371077	35.67996052603351	12.541029326582711	17.23178083367301
26-27	35.65850728337588	38.70754939609122	13.497843949112909	12.136099371419991
28-29	13.443138180335959	39.03471134662004	13.648552978782744	33.87359749426126
30-31	11.875442473129812	38.172290991783406	36.081136163731145	13.87113037135563
32-33	31.132409413682876	40.91830605195975	16.31948168965739	11.629802844699977
34-35	16.420311930148245	16.337180614849935	55.96507412095338	11.27743333404844
36-37	11.561688799262008	54.844142192092335	21.435007401368715	12.15916160727694
38-39	11.797674468495913	20.572587046532085	35.61452813592775	32.01521034904426
40-41	11.668418681483708	13.303155771994938	38.4291935726085	36.59923197391286
42-43	33.66586216143738	13.192276749798873	36.37060874229016	16.771252346473588
44-45	13.284920515735955	34.70598330937722	38.98858687490615	13.020509299980693
46-47	33.74112372085041	14.141977559908181	19.536931757235106	32.57996696200631
48-49	32.34290863063952	12.692811018385427	15.316542595413296	39.64773775556175
50-51	15.281294253958718	12.635175728446981	37.4495773076428	34.63395270995151
52-53	10.845260831312633	12.557133967082251	59.12855947297268	17.46904572863243
54-55	10.470001770756756	13.003793712203734	41.516733651354095	35.009470865685415
56-57	32.62680031767155	12.513146880164847	20.24243920238683	34.61761359977677
58-59	12.282847792263533	13.711572509254788	56.23102097752025	17.774558720961426
60-61	32.18704063348319	34.253049925428385	20.562452386828184	12.99745705426024
62-63	11.793108258180121	61.02780624151676	13.219633363197905	13.95945213710521
64-65	9.801691815026793	63.54605287860664	13.052293927381765	13.599961378984805
66-67	9.917483958194733	63.655922055067926	12.682683434555871	13.743910552181472
68-69	10.162518772795536	63.69502252735465	12.520918257884576	13.621540441965244
70-71	10.318358589245005	59.56229046533459	13.252514415984981	16.86683652943543
72-73	11.688026049685377	50.887545396512046	14.871816879724054	22.552611674078523
74-75	13.124356333676623	46.52849296258153	16.086830587023687	24.26032011671816
76	12.893985881949064	47.28366983500333	15.579204840474606	24.243139442573003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	7.0
16	16.5
17	19.0
18	12.5
19	18.0
20	22.5
21	15.0
22	37.5
23	58.5
24	94.5
25	132.0
26	151.5
27	183.0
28	280.5
29	366.0
30	419.5
31	514.5
32	668.0
33	780.0
34	893.5
35	1219.5
36	6656.0
37	11880.0
38	14281.5
39	15005.5
40	11071.0
41	7151.5
42	5489.0
43	4673.5
44	3568.0
45	3102.5
46	2927.0
47	2881.0
48	2695.5
49	2469.0
50	2382.0
51	2280.0
52	1958.5
53	1661.5
54	1584.0
55	1494.0
56	1256.0
57	1024.5
58	941.0
59	853.5
60	709.5
61	619.0
62	585.0
63	518.0
64	424.5
65	375.5
66	322.5
67	292.0
68	270.0
69	209.0
70	188.0
71	206.0
72	174.5
73	165.5
74	155.5
75	123.0
76	98.5
77	89.0
78	73.0
79	42.0
80	38.5
81	33.5
82	31.0
83	30.0
84	19.5
85	11.0
86	10.0
87	7.0
88	4.5
89	1.5
90	0.5
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04505180958101817
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.001072662132881385
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01501726986033939
52-53	0.025743891189153243
54-55	0.048806127046103015
56-57	0.051487782378306486
58-59	0.03325252611932293
60-61	0.028961877587797395
62-63	0.02949820865423809
64-65	0.013408276661017312
66-67	0.03432518825220432
68-69	0.006435972797288311
70-71	0.014480938793898697
72-73	0.020916911591187005
74-75	0.01072662132881385
76	0.012871945594576622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	93226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.74318323214554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	41.110897559100515	6.8832729066998475
2	13.613940675251456	4.558814064745887
3	11.647126657697482	5.850299272735074
4	10.019860336985072	6.710574303305945
5	7.6302133384585815	6.3877030013086475
6	5.349477865334102	5.374037285735739
7	3.542827855724261	4.152275116383841
8	2.2166698699468257	2.9691287838156737
9	1.358190787366263	2.0466393495376827
>10	3.286565443013646	7.826142921502585
>50	0.0832852841309501	0.9074721644176517
>100	0.10891152540201167	3.363868448716023
>500	0.012813120635530783	1.7237680475403856
>1k	0.012813120635530783	6.855383691244931
>5k	0.0	0.0
>10k+	0.006406560317765392	34.39062064231008
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	32061	34.39062064231008	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	4124	4.423658636002832	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	2267	2.4317250552421	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	832	0.8924548945573124	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	775	0.8313131529830734	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	343	0.3679231115783151	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	337	0.36148713878102673	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	312	0.3346705854589921	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	256	0.27460150601763456	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	247	0.2649475468217021	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT	224	0.24027631776543024	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC	167	0.1791345761911913	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAGTTAATCTCGTATGCCGT	165	0.17698925192542853	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	145	0.15553600926780084	No Hit
CTTATACACATCTCCGAGCCCACGAGACATACGTTAATCTCGTATGCCGT	134	0.1437367258061056	No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT	125	0.13408276661017313	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT	124	0.13301010447729172	No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	117	0.12550146954712205	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT	114	0.1222834831484779	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	111	0.11906549674983373	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCG	109	0.11692017248407097	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	106	0.1137021860854268	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06865037650440864	0.0
2	0.0	0.0	0.0	0.07615901143457833	0.0
3	0.0	0.0	0.0	0.07615901143457833	0.0
4	0.0	0.0	0.0	0.09010361916203634	0.0
5	0.0	0.0	0.0	0.1008302404908502	0.0
6	0.0	0.0	0.0	0.11048419968678266	0.0
7	0.0	0.0	0.0	0.11048419968678266	0.0
8	0.0	0.0	0.0	0.11048419968678266	0.0
9	0.0	0.0	0.0	0.11048419968678266	0.0
10	0.0	0.0	0.0	0.11262952395254543	0.0
11	0.0	0.0	0.0	0.11262952395254543	0.0
12	0.0	0.0	0.0	0.11262952395254543	0.0
13	0.0	0.0	0.0	0.11692017248407097	0.0
14	0.0	0.0	0.0	0.11906549674983374	0.0
15	0.0	0.0	0.0	0.11906549674983374	0.0
16	0.0	0.0	0.0	0.11906549674983374	0.0
17	0.0	0.0	0.0	0.12335614528135928	0.0
18	0.0	0.0	0.0	0.12550146954712205	0.0
19	0.0	0.0	0.0	0.12550146954712205	0.0
20	0.0	0.0	0.0	0.12764679381288482	0.0
21	0.0	0.0	0.0	0.13837341514169868	0.0
22	0.0	0.0	0.0	0.14480938793898698	0.0
23	0.0	0.0	0.0	0.14695471220474976	0.0
24	0.0	0.0	0.0	0.16197198206508914	0.0
25	0.0	0.0	0.0	0.16840795486237745	0.0
26	0.0	0.0	0.0	0.17055327912814022	0.0
27	0.0	0.0	0.0	0.18235256258983545	0.0
28	0.0	0.0	0.0	0.20595112951322592	0.0
29	0.0	0.0	0.0	0.228477034303735	0.0
30	0.0	0.0	0.0	0.2649475468217021	0.0
31	0.0	0.0	0.0	0.28425546521356704	0.0
32	0.0	0.0	0.0	0.32501662626305966	0.0
33	0.0	0.0	0.0	0.359341814515264	0.0
34	0.0	0.0	0.0	0.3861583678372986	0.0
35	0.0	0.0	0.0	0.4784073112650977	0.0
36	0.0	0.0	0.0	0.5921094973505245	0.0
37	0.0	0.0	0.0	0.8688563276339218	0.0
38	0.0	0.0	0.0	1.111277969665115	0.0
39	0.0	0.0	0.0	1.459893162851565	0.0
40	0.0	0.0	0.0	1.8707227597451355	0.0
41	0.0	0.0	0.0	2.2976422886319265	0.0
42	0.0	0.0	0.0	2.77283161349838	0.0
43	0.0	0.0	0.0	3.209405101581104	0.0
44	0.0	0.0	0.0	3.6824491021817947	0.0
45	0.0	0.0	0.0	4.199472250230622	0.0
46	0.0	0.0	0.0	4.703623452684873	0.0
47	0.0	0.0	0.0	5.209919979404887	0.0
48	0.0	0.0	0.0	5.694763263467273	0.0
49	0.0	0.0	0.0	6.007980606268638	0.0
50	0.0	0.0	0.0	6.3877030013086475	0.0
51	0.0	0.0	0.0	6.869328298972389	0.0
52	0.0	0.0	0.0	7.215798167893077	0.0
53	0.0	0.0	0.0	7.613755819192071	0.0
54	0.0	0.0	0.0	7.999914187029369	0.0
55	0.0	0.0	0.0	8.354965353013108	0.0
56	0.0	0.0	0.0	8.758286314976509	0.0
57	0.0	0.0	0.0	9.193787140926352	0.0
58	0.0	0.0	0.0	9.586381481560938	0.0
59	0.0	0.0	0.0	9.988629781391458	0.0
60	0.0	0.0	0.0	10.300774462059941	0.0
61	0.0	0.0	0.0	10.621500439791474	0.0
62	0.0	0.0	0.0	10.888593310878939	0.0
63	0.0	0.0	0.0	11.277969665114883	0.0
64	0.0	0.0	0.0	11.606204277776586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATTAC	15	0.0022138252	70.03703	1
TGTTTCG	15	0.0022185454	69.999466	54
GAGTCGG	15	0.0022185454	69.999466	48
TCGGGCC	15	0.0022185454	69.999466	51
CGTTAAT	15	0.0022185454	69.999466	32
CCACTCG	15	0.0022185454	69.999466	23
CGGATCG	15	0.0022185454	69.999466	51
AGCTGGC	15	0.0022185454	69.999466	17
GACATTA	25	2.8206778E-6	69.999466	26
GACATAC	15	0.0022185454	69.999466	26
GACGCCT	15	0.0022185454	69.999466	28
ATCGGAG	15	0.0022185454	69.999466	54
GCAGAAT	15	0.0022185454	69.999466	25
TGTGTTC	15	0.0022185454	69.999466	10
AGTCGGG	15	0.0022185454	69.999466	49
AACACGA	15	0.0022185454	69.999466	69
TCTTTAT	120	0.0	64.200615	1
AGACATT	35	2.9463263E-7	59.99954	25
CGTCTGC	30	8.3204595E-6	58.332886	41
TCAGTTA	30	8.3204595E-6	58.332886	30
>>END_MODULE