FastQCFastQC Report
Wed 25 May 2016
SRR1779844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284905
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT150.002222673570.00403625
GACGAAT653.6379788E-1253.8492623
ATCTCCG200.00694993552.49381642
TCGTCAG200.00694993552.4938168
CGATCGG200.00694993552.49381643
TCGCGAT200.00694993552.49381640
TCGGTGG1350.049.25345645
CGACGAA2100.048.32763722
AACTTCG305.875331E-446.6611743
AATCTCG305.875331E-446.6611741
GTACATC404.5845027E-543.75252538
GCCCCGT404.5892564E-543.7448466
CTCGGTG1200.043.74484644
GTCGCCG1050.043.33583551
TGGTCGC1050.043.33583549
CGGTGGT1100.041.35876546
ACGAATA602.5894587E-740.8356924
ATGTCGA350.001254906639.9952912
GCCGTAT459.1813614E-538.89113254
TCTCACG459.190862E-538.88430411
Produced by FastQC (version 0.11.5)