FastQCFastQC Report
Wed 25 May 2016
SRR1779844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284905
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT216277.590951369754831No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG26820.9413664203857426No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC14870.5219283620856074No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5990.21024552043663677Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5220.18321896772608412No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT3600.12635790877660974No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3560.1249539320124252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA30550.054.05535538
CGTCTTC31800.053.97725348
CCGTCTT31850.053.97404547
TATGCCG31900.053.96129643
CGTATGC32250.053.91810641
CTCGTAT31800.053.91094239
TCGTATG32200.053.89317340
GCCGTCT31900.053.8799946
ATCTCGT30750.053.70377737
ATGCCGT32150.053.43286544
GTATGCC32400.053.34453242
GTCTTCT32600.053.08247849
TGCCGTC32400.053.0205845
GATCTCG30950.053.01760536
CTTGAAA31900.052.83830357
CTGCTTG32800.052.7773554
AGATCTC30850.052.73582535
GCTTGAA32300.052.5094356
GGTGCAC200.00694235452.5082971
CGTGCGA200.00695683152.4806511