##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779844_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284905
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.535515347221	34.0	34.0	34.0	34.0	34.0
2	33.61168108667802	34.0	34.0	34.0	34.0	34.0
3	33.74598901388182	34.0	34.0	34.0	34.0	34.0
4	33.567073234937965	34.0	34.0	34.0	34.0	34.0
5	33.58400519471403	34.0	34.0	34.0	34.0	34.0
6	37.25449886804373	38.0	38.0	38.0	36.0	38.0
7	37.33537494954459	38.0	38.0	38.0	37.0	38.0
8	37.21982766185219	38.0	38.0	38.0	36.0	38.0
9	37.135736473561366	38.0	38.0	38.0	36.0	38.0
10-11	37.098180095119424	38.0	37.5	38.0	35.5	38.0
12-13	37.24817044277917	38.0	38.0	38.0	36.5	38.0
14-15	37.2679928397185	38.0	38.0	38.0	36.0	38.0
16-17	37.19955774731928	38.0	38.0	38.0	36.0	38.0
18-19	37.07729418578123	38.0	38.0	38.0	36.0	38.0
20-21	37.08796616415998	38.0	38.0	38.0	36.0	38.0
22-23	37.06241729699373	38.0	38.0	38.0	35.5	38.0
24-25	37.25309138133764	38.0	38.0	38.0	36.0	38.0
26-27	37.16982327442481	38.0	38.0	38.0	36.0	38.0
28-29	36.70201997156947	38.0	37.0	38.0	34.0	38.0
30-31	36.969277478457734	38.0	38.0	38.0	35.5	38.0
32-33	37.026756287183446	38.0	38.0	38.0	36.0	38.0
34-35	37.0617521630017	38.0	38.0	38.0	36.0	38.0
36-37	37.06224355486917	38.0	38.0	38.0	36.0	38.0
38-39	36.612806023060315	38.0	37.0	38.0	34.0	38.0
40-41	36.86174865305979	38.0	37.0	38.0	35.0	38.0
42-43	37.1637317702392	38.0	38.0	38.0	36.0	38.0
44-45	36.92989593022236	38.0	38.0	38.0	35.5	38.0
46-47	37.072919043189835	38.0	38.0	38.0	36.0	38.0
48-49	36.72777768027939	38.0	37.0	38.0	34.5	38.0
50-51	36.90456116951265	38.0	37.5	38.0	35.5	38.0
52-53	36.84010635123988	38.0	37.5	38.0	35.0	38.0
54-55	36.82927993541706	38.0	37.0	38.0	35.5	38.0
56-57	36.716493217037254	38.0	37.0	38.0	34.5	38.0
58-59	36.82481177936505	38.0	37.0	38.0	35.0	38.0
60-61	36.512237412470824	38.0	37.0	38.0	34.0	38.0
62-63	36.31926782611748	38.0	37.0	38.0	34.0	38.0
64-65	36.663982731085795	38.0	37.0	38.0	34.0	38.0
66-67	36.451615450764294	38.0	37.0	38.0	34.0	38.0
68-69	36.553540653902175	38.0	37.0	38.0	34.0	38.0
70-71	35.94582404661203	38.0	37.0	38.0	31.5	38.0
72-73	34.88152717572524	38.0	37.0	38.0	23.5	38.0
74-75	34.75235429353644	38.0	37.0	38.0	23.5	38.0
76	34.5108860848353	38.0	36.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	10.0
23	18.0
24	61.0
25	158.0
26	259.0
27	486.0
28	823.0
29	1465.0
30	2368.0
31	3733.0
32	5835.0
33	9313.0
34	15985.0
35	29880.0
36	45041.0
37	169469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.987550701480224	17.11436146863093	10.99998244104581	32.89810538884304
2	19.67111844298977	18.352433267229426	37.56971622119654	24.406732068584265
3	18.48405608887173	19.773959740966287	35.35669784665064	26.385286323511348
4	13.342693178427897	23.2354644530633	33.733349713062246	29.688492655446552
5	13.344799143574173	31.723907969323108	40.094417437391414	14.836875449711307
6	27.768554430424174	41.25480423298995	17.185026587809972	13.791614748775908
7	26.286305961636337	27.98617082887278	19.91821835348625	25.809304856004633
8	23.538021445745073	39.62057527947913	17.749425247012162	19.09197802776364
9	22.545409873466593	16.53814429371194	18.51564556606588	42.40080026675558
10-11	23.372878678857862	27.344026956353872	27.71327284533441	21.569821519453853
12-13	23.51099489303452	20.683034695775785	28.313823906214353	27.49214650497534
14-15	21.09668134992366	21.354311086151522	21.798845229111457	35.75016233481335
16-17	26.15784208771345	28.277496007441076	22.329372948877698	23.23528895596778
18-19	26.31754444463944	24.52764956739966	23.515908811709167	25.638897176251735
20-21	21.283059265369157	23.65455151717239	23.264772468015654	31.7976167494428
22-23	21.469788175005704	28.41964865481476	23.144205963391308	26.966357206788228
24-25	26.269023951927668	28.448705528879902	22.816457473394546	22.465813045797884
26-27	25.987258910865023	28.891209350485248	23.72878678857865	21.392744950071076
28-29	21.36712237412471	24.33495375651533	28.372264439023535	25.925659430336427
30-31	25.23911479265018	23.345852126147314	29.793966409855916	21.62106667134659
32-33	21.828855232445903	23.67455818606202	28.53231779014057	25.964268791351504
34-35	21.077552166511644	32.61525771748477	24.86618346466366	21.44100665133992
36-37	25.907232235306505	29.180253066811744	23.477650444885136	21.434864252996615
38-39	20.97541285691722	24.637686246292624	28.697109562836737	25.689791333953423
40-41	20.58142187746793	22.717923518365772	29.6546217160106	27.0460328881557
42-43	25.382058517956924	22.241351472776795	29.43798612866088	22.938603880605395
44-45	20.507537600252714	26.62220740246749	30.880293431143713	21.989961566136078
46-47	24.43797055158737	22.04313718607957	26.92880082834629	26.590091433986768
48-49	23.836015513943245	21.934679981046315	26.315613976588686	27.913690528421753
50-51	19.70301461231296	22.367457984115145	31.03216464083549	26.89736276273641
52-53	18.222147319931675	22.535641494230475	35.72189715798473	23.52031402785312
54-55	17.89251148351571	22.71646602705474	31.531205663796374	27.85981682563317
56-57	22.445378195527006	22.455562442603867	26.80880129656001	28.29025806530912
58-59	18.211583938876295	22.255481487853736	34.97442937926506	24.558505194004905
60-61	22.59698353426134	26.820772918872134	26.707888070195768	23.874355476670758
62-63	18.186988929811566	33.33836576913759	24.208532436389426	24.266112864661416
64-65	18.098556321032078	34.21054017289043	23.00372986967397	24.68717363640353
66-67	17.866297279456173	33.88126939985112	22.258915153302716	25.99351816738999
68-69	18.085558231127273	34.066209869349414	22.305375559003377	25.542856340519936
70-71	18.638861634215566	32.780672916421665	22.68253854025335	25.89792690910942
72-73	19.411735793679213	30.62951572472774	22.85158581369059	27.107162667902458
74-75	19.564706088849103	29.825706913801277	23.264879855370086	27.344707141979534
76	19.296576611997303	30.01291844529319	23.692007414064257	26.99849752864525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	2.0
14	2.5
15	20.5
16	33.5
17	31.0
18	49.0
19	80.0
20	131.5
21	170.0
22	206.5
23	327.5
24	519.0
25	626.0
26	827.0
27	1147.0
28	1415.0
29	1564.0
30	1933.0
31	2598.0
32	3476.0
33	4058.0
34	4477.5
35	5518.0
36	6761.0
37	7383.0
38	8209.5
39	13168.0
40	19760.0
41	21675.0
42	21130.0
43	19705.5
44	17634.5
45	16349.0
46	15710.0
47	15787.0
48	15345.0
49	14114.0
50	13402.0
51	12871.5
52	11520.0
53	10182.0
54	9665.0
55	8865.0
56	7507.0
57	6256.5
58	5564.0
59	5116.5
60	4171.0
61	3509.0
62	3345.0
63	3142.0
64	2678.0
65	2380.5
66	2239.0
67	2134.0
68	1996.5
69	1723.0
70	1448.5
71	1310.0
72	1297.0
73	1190.5
74	1044.5
75	992.0
76	904.5
77	785.0
78	714.0
79	675.0
80	594.0
81	431.0
82	328.0
83	307.0
84	267.5
85	195.0
86	123.5
87	85.0
88	64.0
89	30.0
90	22.0
91	18.5
92	10.0
93	8.5
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05264912865692073
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.509941910461382E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.509941910461382E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014917253119460874
52-53	0.03141398009862937
54-55	0.05229813446587459
56-57	0.05317561994348993
58-59	0.03702988715536758
60-61	0.03562591039118303
62-63	0.030185500429967885
64-65	0.014917253119460874
66-67	0.03825836682402906
68-69	0.00807286639406118
70-71	0.014215264737368595
72-73	0.023516610800091257
74-75	0.014039767641845526
76	0.014390761832891666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	284905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.67517242589635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.88882088693587	11.151436443726856
2	17.977404311051814	10.310103367789262
3	14.559898160275164	12.52522770748144
4	10.393282495073258	11.921166704691037
5	6.493506493506493	9.310120917498816
6	3.745547572126271	6.444253347607097
7	2.1689902934012264	4.353731945736298
8	1.1493690098779636	2.63666836313859
9	0.8458082916141351	2.1828328741159333
>10	3.694138095646107	17.85296853337077
>50	0.06364982802306082	1.125287376493919
>100	0.013464386697185943	0.7384917779610747
>500	0.0024480703085792625	0.3934644881627209
>1k	0.0024480703085792625	1.46329478247135
>5k	0.0	0.0
>10k+	0.0012240351542896313	7.590951369754831
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	21627	7.590951369754831	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2682	0.9413664203857426	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1487	0.5219283620856074	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	599	0.21024552043663677	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	522	0.18321896772608412	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	360	0.12635790877660974	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	356	0.1249539320124252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004211930292553658	0.0
2	0.0	0.0	0.0	0.004211930292553658	0.0
3	0.0	0.0	0.0	0.004562924483599797	0.0
4	0.0	0.0	0.0	0.009476843158245731	0.0
5	0.0	0.0	0.0	0.010880819922430283	0.0
6	0.0	0.0	0.0	0.01158280830452256	0.0
7	0.0	0.0	0.0	0.01158280830452256	0.0
8	0.0	0.0	0.0	0.012635790877660974	0.0
9	0.0	0.0	0.0	0.013337779259753251	0.0
10	0.0	0.0	0.0	0.016847721170214634	0.0010529825731384146
11	0.0	0.0	0.0	0.01719871536126077	0.0010529825731384146
12	0.0	0.0	0.0	0.01754970955230691	0.0010529825731384146
13	0.0	0.0	0.0	0.018251697934399187	0.0010529825731384146
14	0.0	0.0	0.0	0.018251697934399187	0.0010529825731384146
15	0.0	0.0	0.0	0.018251697934399187	0.0010529825731384146
16	0.0	0.0	0.0	0.019655674698583737	0.0010529825731384146
17	0.0	0.0	0.0	0.020006668889629875	0.0010529825731384146
18	0.0	0.0	0.0	0.022112634035906707	0.0010529825731384146
19	0.0	0.0	0.0	0.026324564328460365	0.0010529825731384146
20	0.0	0.0	0.0	0.027377546901598777	0.0010529825731384146
21	0.0	0.0	0.0	0.028079535283691056	0.0010529825731384146
22	0.0	0.0	0.0	0.030536494621014022	0.0010529825731384146
23	0.0	0.0	0.0	0.03580140748670609	0.0010529825731384146
24	0.0	0.0	0.0	0.03720538425089065	0.0010529825731384146
25	0.0	0.0	0.0	0.03966234358821361	0.0010529825731384146
26	0.0	0.0	0.0	0.04457626226285955	0.0010529825731384146
27	0.0	0.0	0.0	0.1147751004720872	0.0010529825731384146
28	0.0	0.0	0.0	0.1618083220722697	0.0010529825731384146
29	0.0	0.0	0.0	0.20743756690826767	0.0010529825731384146
30	0.0	0.0	0.0	0.25903371299204997	0.0010529825731384146
31	0.0	0.0	0.0	0.3067689229743248	0.0010529825731384146
32	0.0	0.0	0.0	0.35239816781032274	0.0010529825731384146
33	0.0	0.0	0.0	0.4025903371299205	0.0010529825731384146
34	0.0	0.0	0.0	0.47910707077797865	0.0010529825731384146
35	0.0	0.0	0.0	0.576683455888805	0.0010529825731384146
36	0.0	0.0	0.0	0.7813130692687036	0.0010529825731384146
37	0.0	0.0	0.0	1.2467313665958828	0.0010529825731384146
38	0.0	0.0	0.0	1.7454941120724452	0.0010529825731384146
39	0.0	0.0	0.0	2.380091609483863	0.0010529825731384146
40	0.0	0.0	0.0	3.147364911110721	0.0010529825731384146
41	0.0	0.0	0.0	4.2414138046015335	0.0010529825731384146
42	0.0	0.0	0.0	5.460065635913725	0.0010529825731384146
43	0.0	0.0	0.0	6.680472438181148	0.0010529825731384146
44	0.0	0.0	0.0	7.652024358996859	0.0010529825731384146
45	0.0	0.0	0.0	8.544251592636142	0.0010529825731384146
46	0.0	0.0	0.0	9.508081641248838	0.0010529825731384146
47	0.0	0.0	0.0	10.397500921359752	0.0010529825731384146
48	0.0	0.0	0.0	11.24480089854513	0.0010529825731384146
49	0.0	0.0	0.0	11.983292676506204	0.0010529825731384146
50	0.0	0.0	0.0	12.843228444569242	0.0010529825731384146
51	0.0	0.0	0.0	13.775469015987785	0.0010529825731384146
52	0.0	0.0	0.0	14.948842596655025	0.0010529825731384146
53	0.0	0.0	0.0	16.095891612993803	0.0010529825731384146
54	0.0	0.0	0.0	17.134834418490374	0.0010529825731384146
55	0.0	0.0	0.0	17.976167494427965	0.0010529825731384146
56	0.0	0.0	0.0	18.720626173636827	0.0010529825731384146
57	0.0	0.0	0.0	19.42472052087538	0.0010529825731384146
58	0.0	0.0	0.0	20.097576385110827	0.0010529825731384146
59	0.0	0.0	0.0	20.66899492813394	0.0010529825731384146
60	0.0	0.0	0.0	21.28428774503782	0.0010529825731384146
61	0.0	0.0	0.0	21.84236850880118	0.0010529825731384146
62	0.0	0.0	0.0	22.53593303030835	0.0010529825731384146
63	0.0	0.0	0.0	23.255471121952933	0.0010529825731384146
64	0.0	0.0	0.0	23.95921447500044	0.0010529825731384146
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	3055	0.0	54.055355	38
CGTCTTC	3180	0.0	53.977253	48
CCGTCTT	3185	0.0	53.974045	47
TATGCCG	3190	0.0	53.961296	43
CGTATGC	3225	0.0	53.918106	41
CTCGTAT	3180	0.0	53.910942	39
TCGTATG	3220	0.0	53.893173	40
GCCGTCT	3190	0.0	53.87999	46
ATCTCGT	3075	0.0	53.703777	37
ATGCCGT	3215	0.0	53.432865	44
GTATGCC	3240	0.0	53.344532	42
GTCTTCT	3260	0.0	53.082478	49
TGCCGTC	3240	0.0	53.02058	45
GATCTCG	3095	0.0	53.017605	36
CTTGAAA	3190	0.0	52.838303	57
CTGCTTG	3280	0.0	52.77735	54
AGATCTC	3085	0.0	52.735825	35
GCTTGAA	3230	0.0	52.50943	56
GGTGCAC	20	0.006942354	52.508297	1
CGTGCGA	20	0.006956831	52.48065	11
>>END_MODULE