FastQCFastQC Report
Wed 25 May 2016
SRR1779843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117053
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC150.002219902170.038
GCGCGAA150.002219902170.026
GCCGGTT150.002219902170.024
ATTCGGT150.002219902170.011
GTGTCGA207.898952E-570.036
CCGGTTA150.002219902170.025
TGTCGAT207.898952E-570.037
CTAATGG150.002219902170.01
GATCTCG850.057.6470641
TTCCGAC553.45608E-1157.27272419
CTTCCGA509.695214E-1055.99999618
GAAACTC252.382145E-455.99999626
CGAGCGA252.382145E-455.99999625
CTCGGTG850.053.5294144
CGGTGGT850.053.5294146
CTTCGTG200.00693652752.536
ATCTCGG800.052.542
AGATCCG200.00693652752.554
CGGCCAT200.00693652752.52
GATCCGC200.00693652752.555
Produced by FastQC (version 0.11.5)