##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779843_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117053
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.61986450582215	33.0	23.0	34.0	12.0	34.0
2	27.99203779484507	34.0	23.0	34.0	12.0	34.0
3	27.7407499167044	34.0	23.0	34.0	11.0	34.0
4	27.840790069455718	34.0	23.0	34.0	12.0	34.0
5	28.045552014899233	34.0	23.0	34.0	11.0	34.0
6	30.939634182806078	37.0	26.0	38.0	12.0	38.0
7	31.100108497859942	37.0	26.0	38.0	12.0	38.0
8	30.501140509000198	37.0	25.0	38.0	11.0	38.0
9	31.352207974165548	37.0	27.0	38.0	11.0	38.0
10-11	30.01699657420143	36.0	24.0	38.0	11.0	38.0
12-13	30.68896568221233	37.0	26.0	38.0	11.0	38.0
14-15	29.600108497859942	35.0	22.0	38.0	11.0	38.0
16-17	30.135891433794946	36.5	24.0	38.0	11.0	38.0
18-19	30.794798082919705	37.0	26.0	38.0	11.0	38.0
20-21	29.76546948818057	35.0	23.5	38.0	11.0	38.0
22-23	30.498722800782552	37.0	24.5	38.0	11.0	38.0
24-25	30.781445157321897	37.0	25.5	38.0	11.0	38.0
26-27	29.790641846001385	36.0	22.5	38.0	11.0	38.0
28-29	30.788762355514166	37.0	25.5	38.0	11.0	38.0
30-31	31.163669448882132	37.0	26.0	38.0	11.0	38.0
32-33	31.271116502780792	37.0	26.0	38.0	11.0	38.0
34-35	31.265486574457725	37.0	26.0	38.0	11.0	38.0
36-37	30.865129471265156	37.0	25.5	38.0	11.0	38.0
38-39	30.720169495869392	37.0	25.0	38.0	11.0	38.0
40-41	30.682438724338546	37.0	25.0	38.0	11.0	38.0
42-43	31.085683408370567	37.0	26.0	38.0	11.0	38.0
44-45	31.167894030909075	37.0	26.0	38.0	11.0	38.0
46-47	31.048443867307974	37.0	26.0	38.0	11.0	38.0
48-49	30.7568152888008	37.0	25.0	38.0	11.0	38.0
50-51	30.195022767464312	36.5	23.5	38.0	11.0	38.0
52-53	30.718055069071276	37.0	25.0	38.0	11.0	38.0
54-55	30.8402860242796	37.0	25.5	38.0	11.0	38.0
56-57	30.864343502515954	37.0	25.5	38.0	11.0	38.0
58-59	31.066538234816708	37.0	25.5	38.0	11.0	38.0
60-61	30.999431881284544	37.0	25.0	38.0	11.0	38.0
62-63	30.936678256858002	37.0	25.0	38.0	11.0	38.0
64-65	30.971111376897646	37.0	25.0	38.0	11.0	38.0
66-67	30.937502669730804	37.0	25.0	38.0	11.0	38.0
68-69	30.40856705936627	37.0	24.0	38.0	11.0	38.0
70-71	30.459471350584778	37.0	24.5	38.0	11.0	38.0
72-73	30.53916601881199	37.0	24.0	38.0	11.0	38.0
74-75	30.743880976993328	37.0	25.0	38.0	11.0	38.0
76	29.73298420373677	36.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	29.0
13	397.0
14	1626.0
15	3097.0
16	4055.0
17	4792.0
18	4749.0
19	4036.0
20	3318.0
21	2382.0
22	1794.0
23	1459.0
24	1277.0
25	1267.0
26	1340.0
27	1381.0
28	1733.0
29	1947.0
30	2328.0
31	2961.0
32	3547.0
33	4737.0
34	6626.0
35	9453.0
36	16597.0
37	30124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.04742296224787	20.706004972106655	10.477305152366876	35.7692669132786
2	16.379759596080408	20.49584376308168	32.32723638010132	30.79716026073659
3	19.381818492477766	21.947323007526506	31.683083731301203	26.98777476869452
4	16.0696436656899	24.27874552553117	30.185471538533825	29.4661392702451
5	12.060348730916765	32.29733539507744	32.50066209323981	23.141653780765978
6	23.473075378687557	43.985852079043816	15.22781057505831	17.313261967210313
7	22.224690493075077	33.59421057578113	18.970275373587032	25.210823557556754
8	20.121143774935714	38.958231881829285	19.645282825435068	21.275341517799934
9	22.714922848208335	18.471147109584592	19.103398779925154	39.71053126228192
10-11	20.98181103640356	28.231339011866822	24.72426077967347	26.06258917205615
12-13	20.829026167633465	23.406491076691754	26.215475041220643	29.549007714454135
14-15	24.848464137580468	25.530642494970163	22.282926745919532	27.33796662152984
16-17	26.593081766379335	25.438476587528726	22.108361169726535	25.860080476365404
18-19	21.952626070612357	25.383711740959868	24.49347487131293	28.17018731711485
20-21	24.278318368602257	24.940838765345614	23.691831905205333	27.089010960846792
22-23	26.268688594617686	24.653139683895773	22.661255873558307	26.416915847928234
24-25	21.369529464533628	29.213780730045496	22.41953053247613	26.997159272944742
26-27	23.35575916789475	29.44533435851434	23.216932572990753	23.98197390060016
28-29	22.070859818194243	27.78091039573508	25.771478367849088	24.376751418221584
30-31	22.887361545986167	26.331572000358815	25.923631905614197	24.857434548040818
32-33	20.26389755068217	28.63959061279933	25.53800415196535	25.55850768455315
34-35	20.641192678186208	29.99722334949486	24.925350819111898	24.43623315320703
36-37	24.11839598055216	26.1648623014415	24.38029240115867	25.33644931684767
38-39	24.155637767961654	25.027981647456876	26.85255594193488	23.963824642646596
40-41	21.21740244761966	27.517139744986224	25.868734115033853	25.39672369236027
42-43	19.227779106721798	26.99868434541913	26.648412671291393	27.12512387656768
44-45	18.56751688402671	25.0579887824278	28.979867319957116	27.394627013588384
46-47	20.067833969098807	26.234830265569137	27.466777160285517	26.23055860504654
48-49	20.52104602188752	27.202207547008623	26.416238797809537	25.860507633294322
50-51	23.71038017941051	26.623665100384454	23.85689876121316	25.809055958991884
52-53	17.956604765017236	28.664436669358047	25.89530974320855	27.483648822416175
54-55	18.872583573640927	26.557727224180283	25.959628324255046	28.610060877923743
56-57	20.31579756829037	27.321274468757746	24.735340106120287	27.6275878568316
58-59	19.867818449024234	26.982723264636522	25.300334939676684	27.849123346662562
60-61	18.700461892777636	26.120228852702777	25.65107226635105	29.528236988168537
62-63	19.517936405541768	26.609192701546924	24.89608120404824	28.976789688863064
64-65	20.012988511149185	25.754423143476053	24.5914386912366	29.641149654138164
66-67	18.65902167457798	29.763771453474213	22.771338115725925	28.80586875622188
68-69	19.055897755717496	31.556218123414183	21.97337958019017	27.414504540678152
70-71	18.836500017086426	31.214759252298123	23.278970030413834	26.669770700201617
72-73	19.537961408500042	29.337058348605044	24.118619523010086	27.00636071988483
74-75	19.086637047163364	29.619788106630214	23.03144224196856	28.262132604237866
76	20.685707450853076	29.35743761053252	22.49267388314096	27.464181055473443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	4.0
16	8.5
17	12.0
18	9.5
19	11.5
20	22.0
21	28.0
22	34.5
23	76.5
24	148.0
25	184.0
26	214.5
27	282.0
28	430.0
29	541.0
30	616.5
31	832.0
32	1193.5
33	1415.0
34	1671.0
35	2160.5
36	2732.5
37	3071.0
38	3473.0
39	4349.5
40	5207.0
41	5843.0
42	6096.0
43	6473.5
44	7199.0
45	7653.5
46	7760.0
47	7812.0
48	7626.5
49	7105.0
50	6821.0
51	6574.5
52	5869.0
53	5095.5
54	4781.0
55	4539.0
56	3896.0
57	3202.0
58	2909.0
59	2635.0
60	2150.0
61	1768.5
62	1598.0
63	1444.0
64	1189.5
65	952.0
66	801.5
67	788.0
68	728.5
69	568.5
70	440.0
71	412.0
72	401.5
73	332.5
74	266.0
75	258.0
76	219.5
77	161.5
78	133.5
79	125.0
80	115.5
81	91.0
82	68.0
83	60.0
84	57.0
85	44.0
86	26.5
87	19.0
88	17.5
89	15.5
90	13.5
91	9.0
92	6.0
93	5.0
94	3.5
95	6.0
96	9.0
97	7.0
98	4.0
99	6.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003417255431300351
7	0.010251766293901053
8	0.0017086277156501755
9	0.009397452436075966
10-11	0.003417255431300351
12-13	0.0
14-15	0.0012814707867376317
16-17	0.0
18-19	0.004698726218037983
20-21	0.0
22-23	0.0025629415734752634
24-25	0.004698726218037983
26-27	4.271569289125439E-4
28-29	0.004271569289125439
30-31	0.0012814707867376317
32-33	0.0
34-35	0.004698726218037983
36-37	0.01879490487215193
38-39	0.010251766293901053
40-41	4.271569289125439E-4
42-43	8.543138578250878E-4
44-45	0.0038444123602128953
46-47	0.0021357846445627196
48-49	0.0
50-51	0.0025629415734752634
52-53	0.010678923222813597
54-55	0.013241864796288862
56-57	0.013669021725201404
58-59	0.014523335583026492
60-61	0.029473828094965526
62-63	0.012387550938463773
64-65	0.022639317232364828
66-67	0.022639317232364828
68-69	0.0
70-71	8.543138578250878E-4
72-73	0.005553040075863071
74-75	0.011106080151726141
76	0.005125883146950527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117053.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.28354676941214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60173333819543	59.46109881848394
2	6.507919143298205	9.14799278959104
3	3.5238060508818636	7.429967621504789
4	1.9168824222975847	5.389011815160654
5	1.1511018731235338	4.045176116801791
6	0.657598852544701	2.773102782500235
7	0.4655459529105738	2.2904154528290603
8	0.28443277540750467	1.5992755418485642
9	0.20542367112764223	1.2994113777519585
>10	0.68555592021296	6.564547683527974
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005125883146950527	0.0
2	0.0	0.0	0.0	0.005125883146950527	0.0
3	0.0	0.0	0.0	0.005125883146950527	0.0
4	0.0	0.0	0.0	0.005125883146950527	0.0
5	0.0	0.0	0.0	0.008543138578250878	0.0
6	0.0	0.0	0.0	0.012814707867376316	0.0
7	0.0	0.0	0.0	0.012814707867376316	0.0
8	0.0	0.0	0.0	0.012814707867376316	0.0
9	0.0	0.0	0.0	0.014523335583026492	0.0
10	0.0	0.0	0.0	0.01623196329867667	0.0
11	0.0	0.0	0.0	0.01623196329867667	0.0
12	0.0	0.0	0.0	0.017940591014326844	0.0
13	0.0	0.0	0.0	0.01964921872997702	0.0
14	0.0	0.0	0.0	0.01964921872997702	0.0
15	0.0	0.0	0.0	0.020503532587802107	0.0
16	0.0	0.0	0.0	0.020503532587802107	0.0
17	0.0	0.0	0.0	0.021357846445627195	0.0
18	0.0	0.0	0.0	0.023920788019102458	0.0
19	0.0	0.0	0.0	0.023920788019102458	0.0
20	0.0	0.0	0.0	0.025629415734752633	0.0
21	0.0	0.0	0.0	0.03246392659735334	0.0
22	0.0	0.0	0.0	0.045278634464729656	0.0
23	0.0	0.0	0.0	0.045278634464729656	0.0
24	0.0	0.0	0.0	0.05723902847428088	0.0
25	0.0	0.0	0.0	0.05894765618993106	0.0
26	0.0	0.0	0.0	0.06236491162123141	0.0
27	0.0	0.0	0.0	0.07090805019948229	0.0
28	0.0	0.0	0.0	0.0914115827872844	0.0
29	0.0	0.0	0.0	0.11618668466421193	0.0
30	0.0	0.0	0.0	0.155485122124166	0.0
31	0.0	0.0	0.0	0.19222061801064474	0.0
32	0.0	0.0	0.0	0.21528709217192213	0.0
33	0.0	0.0	0.0	0.25373121577405106	0.0
34	0.0	0.0	0.0	0.3075529888170316	0.0
35	0.0	0.0	0.0	0.379315352874339	0.0
36	0.0	0.0	0.0	0.4929390959650756	0.0
37	0.0	0.0	0.0	0.7475246255969518	0.0
38	0.0	0.0	0.0	1.0294481986792308	0.0
39	0.0	0.0	0.0	1.353233150794939	0.0
40	0.0	0.0	0.0	1.8162712617361367	0.0
41	0.0	0.0	0.0	2.297249963691661	0.0
42	0.0	0.0	0.0	2.8089839645288888	0.0
43	0.0	0.0	0.0	3.1959881421236536	0.0
44	0.0	0.0	0.0	3.665860763927452	0.0
45	0.0	0.0	0.0	4.166488684612953	0.0
46	0.0	0.0	0.0	4.697017590322333	0.0
47	0.0	0.0	0.0	5.177996292277857	0.0
48	0.0	0.0	0.0	5.707670884129412	0.0
49	0.0	0.0	0.0	6.1578942872032325	0.0
50	0.0	0.0	0.0	6.668773974182635	0.0
51	0.0	0.0	0.0	7.1617130701477105	0.0
52	0.0	0.0	0.0	7.736666296463995	0.0
53	0.0	0.0	0.0	8.183472444106515	0.0
54	0.0	0.0	0.0	8.57560250484823	0.0
55	0.0	0.0	0.0	9.05060100979898	0.0
56	0.0	0.0	0.0	9.553791872057957	0.0
57	0.0	0.0	0.0	10.000598019700478	0.0
58	0.0	0.0	0.0	10.439715342622574	0.0
59	0.0	0.0	0.0	10.833554031079938	0.0
60	0.0	0.0	0.0	11.282923120295933	0.0
61	0.0	0.0	0.0	11.692139458194152	0.0
62	0.0	0.0	0.0	12.082560891220217	0.0
63	0.0	0.0	0.0	12.507154878559286	0.0
64	0.0	0.0	0.0	12.8890331730071	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	15	0.0022199021	70.0	38
GCGCGAA	15	0.0022199021	70.0	26
GCCGGTT	15	0.0022199021	70.0	24
ATTCGGT	15	0.0022199021	70.0	11
GTGTCGA	20	7.898952E-5	70.0	36
CCGGTTA	15	0.0022199021	70.0	25
TGTCGAT	20	7.898952E-5	70.0	37
CTAATGG	15	0.0022199021	70.0	1
GATCTCG	85	0.0	57.64706	41
TTCCGAC	55	3.45608E-11	57.272724	19
CTTCCGA	50	9.695214E-10	55.999996	18
GAAACTC	25	2.382145E-4	55.999996	26
CGAGCGA	25	2.382145E-4	55.999996	25
CTCGGTG	85	0.0	53.52941	44
CGGTGGT	85	0.0	53.52941	46
CTTCGTG	20	0.006936527	52.5	36
ATCTCGG	80	0.0	52.5	42
AGATCCG	20	0.006936527	52.5	54
CGGCCAT	20	0.006936527	52.5	2
GATCCGC	20	0.006936527	52.5	55
>>END_MODULE