FastQCFastQC Report
Wed 25 May 2016
SRR1779843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117053
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2045517.47498996181217No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG27702.366449386175493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC15861.3549417785105893No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT5110.4365543813486199RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4800.4100706517560422No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT4380.3741894697273884No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT3820.3263478936891835No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2520.21528709217192213No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1890.1614653191289416No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1800.1537764944085158No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1550.1324186479628886No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1190.10166334908118546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAACG150.002219955469.9995721
CCTGAAC150.002219955469.9995719
GCTTGAC401.05610525E-861.24962656
CTCTCGT308.33366E-658.33297737
AACGGGG252.3822166E-455.9996624
GCTCTCG252.3822166E-455.9996636
CTTGACA452.6757334E-854.44411557
CATGGCG200.00693669452.4996821
TGCCCTA200.00693669452.4996831
CGTGCCC200.00693669452.4996833
ATCAGCA200.00693669452.4996818
GTAGGCA200.00693669452.4996852
TCAGCAC200.00693669452.4996819
GGGGTAC200.00693669452.4996827
GATTGAT200.00693669452.4996864
AGTCGCC200.00693669452.4996832
GATCTCG26900.050.48296436
TCTCGTA27150.050.4048538
CCGTCTT29450.050.39018247
GCCGTCT29450.050.39018246
Produced by FastQC (version 0.11.5)