##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779843_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117053
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.521575696479374	34.0	34.0	34.0	34.0	34.0
2	33.60705834109335	34.0	34.0	34.0	34.0	34.0
3	33.749797100458764	34.0	34.0	34.0	34.0	34.0
4	33.55665382348167	34.0	34.0	34.0	34.0	34.0
5	33.60245358939967	34.0	34.0	34.0	34.0	34.0
6	37.29837765798399	38.0	38.0	38.0	37.0	38.0
7	37.352908511528966	38.0	38.0	38.0	37.0	38.0
8	37.25117681733916	38.0	38.0	38.0	36.0	38.0
9	37.17514288399272	38.0	38.0	38.0	36.0	38.0
10-11	37.109215483584364	38.0	37.5	38.0	35.5	38.0
12-13	37.27356838355275	38.0	38.0	38.0	36.5	38.0
14-15	37.28142807104474	38.0	38.0	38.0	36.5	38.0
16-17	37.164297369567635	38.0	38.0	38.0	36.0	38.0
18-19	37.021695300419466	38.0	38.0	38.0	35.5	38.0
20-21	37.07145481106849	38.0	38.0	38.0	35.5	38.0
22-23	37.078490085687676	38.0	38.0	38.0	36.0	38.0
24-25	37.249446831777064	38.0	38.0	38.0	36.0	38.0
26-27	37.09979240173254	38.0	38.0	38.0	36.0	38.0
28-29	36.606114324280455	38.0	37.0	38.0	34.0	38.0
30-31	37.00044424320607	38.0	37.0	38.0	36.0	38.0
32-33	36.808219353626136	38.0	37.0	38.0	35.0	38.0
34-35	36.925884001264386	38.0	38.0	38.0	35.5	38.0
36-37	36.81131196979146	38.0	38.0	38.0	35.0	38.0
38-39	35.41025860080477	38.0	36.5	38.0	29.0	38.0
40-41	36.454755538089586	38.0	37.0	38.0	34.0	38.0
42-43	37.094606716615544	38.0	37.5	38.0	36.0	38.0
44-45	36.850422458202694	38.0	37.5	38.0	35.0	38.0
46-47	37.011772444960826	38.0	38.0	38.0	36.0	38.0
48-49	36.642854091736226	38.0	37.0	38.0	34.0	38.0
50-51	36.761232091445756	38.0	37.0	38.0	34.5	38.0
52-53	36.66778297010756	38.0	37.0	38.0	34.0	38.0
54-55	36.693685766276815	38.0	37.0	38.0	34.5	38.0
56-57	36.54662845036009	38.0	37.0	38.0	34.0	38.0
58-59	36.63801013216235	38.0	37.0	38.0	34.0	38.0
60-61	36.24762714325989	38.0	37.0	38.0	34.0	38.0
62-63	36.1374035693233	38.0	37.0	38.0	34.0	38.0
64-65	36.58400041007065	38.0	37.0	38.0	34.0	38.0
66-67	36.31290099356701	38.0	37.0	38.0	33.5	38.0
68-69	36.333618104619276	38.0	37.0	38.0	34.0	38.0
70-71	34.91803285691097	38.0	35.5	38.0	27.5	38.0
72-73	32.29131248237978	38.0	28.0	38.0	11.0	38.0
74-75	31.885445054804233	38.0	25.0	38.0	11.0	38.0
76	31.67583914978685	38.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	2.0
23	7.0
24	33.0
25	81.0
26	145.0
27	280.0
28	467.0
29	821.0
30	1369.0
31	2019.0
32	3092.0
33	5035.0
34	8797.0
35	17739.0
36	19005.0
37	58160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.44232659515364	16.064789093551006	12.022736014359587	41.47014829693577
2	14.759980521644042	15.490418870084492	48.1713411873254	21.57825942094607
3	13.803149000879944	18.903402732095717	45.2735085815827	22.019939685441635
4	10.322674344100536	33.49337479603257	33.74283444251749	22.441116417349406
5	9.961299582240525	30.388798236696196	48.99062817697966	10.65927400408362
6	21.16904308304785	50.45833938472316	17.072608134776555	11.300009397452436
7	20.039640163003085	25.94038597900096	19.772239925503833	34.24773393249212
8	17.760330790325753	48.77961265409686	15.697162823678163	17.762893731899226
9	17.85601394240216	16.07476954883685	16.38146822379606	49.687748284964925
10-11	18.834630466540798	31.805677769899106	30.40161294456357	18.958078818996523
12-13	18.701784661648997	18.784225948929116	31.976967698393032	30.537021691028848
14-15	17.45106917379307	18.393804515903053	20.089617523685853	44.06550878661803
16-17	29.013352925597808	30.08551681716829	18.704774760151384	22.196355497082518
18-19	29.938147676693465	22.536799569425817	19.583009405995575	27.942043347885146
20-21	18.060194954422357	20.24894705817023	19.48988919549264	42.200968791914775
22-23	17.717615097434493	31.6557456878508	19.653917456195057	30.972721758519643
24-25	28.95874447890236	31.445694611750437	19.330462789724137	20.26509811962307
26-27	29.401638573979312	33.18240455178423	19.727388447968014	17.688568426268443
28-29	18.498457963486626	32.601471128463174	20.637232706551735	28.262838201498468
30-31	17.260557183498072	22.38131444730165	41.82208059596935	18.53604777323093
32-33	28.687005031908626	20.38820021699572	23.64911621231408	27.275678538781577
34-35	28.7442440603829	19.894833964101732	20.325408148445575	31.03551382706979
36-37	31.546820670978104	29.8065833425884	20.009739177979206	18.63685680845429
38-39	18.283170871314702	23.041699059400443	31.003477057401348	27.671653011883507
40-41	16.973507727268846	19.549264008611484	33.290475254799105	30.186753009320565
42-43	28.674739967108774	19.479720638175177	31.995044958458813	19.850494436257236
44-45	17.44466181985938	30.77067653114401	33.74069865787293	18.04396299112368
46-47	28.119740630312762	19.849555329637003	24.11599873561549	27.914705304434744
48-49	27.330354625682386	19.235730822789677	21.198516911142814	32.23539764038513
50-51	18.612539945656817	18.97653673291522	32.85754567048892	29.553377650939044
52-53	15.832428875424098	19.06241186876672	44.32498953107774	20.78016972473144
54-55	15.301303175234116	19.615159402821767	35.396872208473845	29.686665213470278
56-57	26.750811025675	19.200899287494178	24.324358979838692	29.723930706992128
58-59	16.627066203996513	19.445394095827425	42.632604570862036	21.29493512931403
60-61	26.944311500068373	29.966754409954877	24.123136879529604	18.96579721044715
62-63	16.079562449258642	44.7228987736615	20.23202153570055	18.96551724137931
64-65	15.151670511834572	45.89207895411433	19.743655472955652	19.212595061095445
66-67	14.987393701123883	46.077945386949274	19.303875902739197	19.630785009187644
68-69	15.035393731229787	46.14129297123645	18.975482845681626	19.847830451852136
70-71	15.89144802894947	44.09752929766772	19.40588644937474	20.605136224008067
72-73	16.20720890089043	38.88969595460683	20.40171933482593	24.501375809676812
74-75	17.063719249075014	36.359363918345025	20.94779926343043	25.62911756914953
76	17.238462261490742	36.11839800391356	20.949508241546965	25.69363149304873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.5
15	4.5
16	7.5
17	11.0
18	18.5
19	30.0
20	35.5
21	37.0
22	39.0
23	74.0
24	141.5
25	176.0
26	227.0
27	303.5
28	401.5
29	474.0
30	581.0
31	765.5
32	1123.5
33	1404.0
34	1557.0
35	1941.5
36	2471.5
37	2770.0
38	3072.5
39	3795.5
40	4807.0
41	8412.5
42	11427.0
43	13099.5
44	14261.5
45	12013.5
46	10276.0
47	9032.5
48	7047.0
49	5698.0
50	5091.0
51	4590.0
52	3623.0
53	3022.5
54	2888.0
55	2716.0
56	2298.0
57	1795.0
58	1538.0
59	1338.5
60	1049.0
61	877.0
62	795.0
63	747.0
64	654.0
65	517.0
66	440.0
67	455.0
68	399.5
69	345.5
70	307.0
71	267.0
72	258.5
73	218.0
74	180.5
75	175.0
76	142.0
77	101.0
78	99.0
79	105.0
80	86.0
81	73.5
82	65.5
83	51.0
84	43.5
85	32.0
86	26.5
87	25.0
88	19.0
89	10.0
90	5.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04955020375385509
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0017086277156501755
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.271569289125439E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016231963298676665
52-53	0.03417255431300351
54-55	0.05937481311884361
56-57	0.06108344083449377
58-59	0.04357000674907947
60-61	0.03844412360212895
62-63	0.03246392659735333
64-65	0.01964921872997702
66-67	0.041007065175604214
68-69	0.010678923222813597
70-71	0.018367747943239386
72-73	0.026483729592577724
74-75	0.020503532587802107
76	0.020503532587802107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.687560335916206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	41.529916687705125	9.837424072855885
2	16.132289825801564	7.642691772103236
3	12.807011216503769	9.10100552741066
4	9.351895264543586	8.86094333336181
5	5.936451833952465	7.031003049900472
6	3.8698741299094745	5.500072616677915
7	2.477729289140549	4.108395342280847
8	1.6446063403902331	3.1165369533459204
9	1.2118151981822771	2.5834451060630657
>10	4.955458578281098	17.992704159654174
>50	0.03606592851732968	0.6176689192075385
>100	0.03245933566559671	1.9751736392916028
>500	0.003606592851732968	0.4365543813486199
>1k	0.007213185703465936	3.721391164686082
>5k	0.0	0.0
>10k+	0.003606592851732968	17.47498996181217
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	20455	17.47498996181217	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	2770	2.366449386175493	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1586	1.3549417785105893	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	511	0.4365543813486199	RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	480	0.4100706517560422	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	438	0.3741894697273884	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	382	0.3263478936891835	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	252	0.21528709217192213	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	189	0.1614653191289416	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	180	0.1537764944085158	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	155	0.1324186479628886	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	119	0.10166334908118546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010251766293901053	0.0
2	0.0	0.0	0.0	0.010251766293901053	0.0
3	0.0	0.0	0.0	0.010251766293901053	0.0
4	0.0	0.0	0.0	0.010251766293901053	0.0
5	0.0	0.0	0.0	0.014523335583026492	0.0
6	0.0	0.0	0.0	0.01879490487215193	0.0
7	0.0	0.0	0.0	0.01879490487215193	0.0
8	0.0	0.0	0.0	0.01879490487215193	0.0
9	0.0	0.0	0.0	0.021357846445627195	0.0
10	0.0	0.0	0.0	0.02306647416127737	0.0
11	0.0	0.0	0.0	0.02306647416127737	0.0
12	0.0	0.0	0.0	0.025629415734752633	0.0
13	0.0	0.0	0.0	0.027338043450402808	0.0
14	0.0	0.0	0.0	0.027338043450402808	0.0
15	0.0	0.0025629415734752634	0.0	0.027338043450402808	0.0
16	0.0	0.0025629415734752634	0.0	0.027338043450402808	0.0
17	0.0	0.0025629415734752634	0.0	0.028192357308227896	0.0
18	0.0	0.0025629415734752634	0.0	0.03246392659735334	0.0
19	0.0	0.0025629415734752634	0.0	0.03417255431300351	0.0
20	0.0	0.0025629415734752634	0.0	0.03673549588647877	0.0
21	0.0	0.0025629415734752634	0.0	0.03673549588647877	0.0
22	0.0	0.0025629415734752634	0.0	0.03673549588647877	0.0
23	0.0	0.0025629415734752634	0.0	0.03929843745995404	0.0
24	0.0	0.0025629415734752634	0.0	0.041007065175604214	0.0
25	0.0	0.0025629415734752634	0.0	0.0418613790334293	0.0
26	0.0	0.0025629415734752634	0.0	0.045278634464729656	0.0
27	0.0	0.0025629415734752634	0.0	0.05553040075863071	0.0
28	0.0	0.0025629415734752634	0.0	0.07945118877773316	0.0
29	0.0	0.0025629415734752634	0.0	0.10935217380161123	0.0
30	0.0	0.0025629415734752634	0.0	0.15206786669286562	0.0
31	0.0	0.0025629415734752634	0.0	0.18794904872151932	0.0
32	0.0	0.0025629415734752634	0.0	0.21357846445627193	0.0
33	0.0	0.0025629415734752634	0.0	0.2554398434897012	0.0
34	0.0	0.0025629415734752634	0.0	0.31182455810615706	0.0
35	0.0	0.0025629415734752634	0.0	0.38871280531041497	0.0
36	0.0	0.0025629415734752634	0.0	0.5168598839841781	0.0
37	0.0	0.0025629415734752634	0.0	0.8047636540712327	0.0
38	0.0	0.0025629415734752634	0.0	1.0952303657317626	0.0
39	0.0	0.0025629415734752634	0.0	1.431830025714847	0.0
40	0.0	0.0025629415734752634	0.0	1.928186377111223	0.0
41	0.0	0.0025629415734752634	0.0	2.432231553228025	0.0
42	0.0	0.0025629415734752634	0.0	2.9738665390891303	0.0
43	0.0	0.0025629415734752634	0.0	3.3822285631295226	0.0
44	0.0	0.0025629415734752634	0.0	3.8751676590945983	0.0
45	0.0	0.0025629415734752634	0.0	4.401424995514852	0.0
46	0.0	0.0025629415734752634	0.0	4.962709200105935	0.0
47	0.0	0.0025629415734752634	0.0	5.4650457485070865	0.0
48	0.0	0.0025629415734752634	0.0	6.025475639240344	0.0
49	0.0	0.0025629415734752634	0.0	6.514143165916295	0.0
50	0.0	0.0025629415734752634	0.0	7.0557781517774	0.0
51	0.0	0.0025629415734752634	0.0	7.574346663477228	0.0
52	0.0	0.0025629415734752634	0.0	8.148445575935687	0.0
53	0.0	0.0025629415734752634	0.0	8.599523292867334	0.0
54	0.0	0.0025629415734752634	0.0	9.006176689192076	0.0
55	0.0	0.0025629415734752634	0.0	9.480320880285	0.0
56	0.0	0.0025629415734752634	0.0	9.995472136553527	0.0
57	0.0	0.0025629415734752634	0.0	10.455092992063424	0.0
58	0.0	0.0025629415734752634	0.0	10.892501687269869	0.0
59	0.0	0.0025629415734752634	0.0	11.304280966741562	0.0
60	0.0	0.0025629415734752634	0.0	11.764756136109284	0.0
61	0.0	0.0025629415734752634	0.0	12.191913065021827	0.0
62	0.0	0.0025629415734752634	0.0	12.59002332276832	0.0
63	0.0	0.0025629415734752634	0.0	13.041101039699965	0.0
64	0.0	0.0025629415734752634	0.0	13.43664835587298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAAACG	15	0.0022199554	69.99957	21
CCTGAAC	15	0.0022199554	69.99957	19
GCTTGAC	40	1.05610525E-8	61.249626	56
CTCTCGT	30	8.33366E-6	58.332977	37
AACGGGG	25	2.3822166E-4	55.99966	24
GCTCTCG	25	2.3822166E-4	55.99966	36
CTTGACA	45	2.6757334E-8	54.444115	57
CATGGCG	20	0.006936694	52.49968	21
TGCCCTA	20	0.006936694	52.49968	31
CGTGCCC	20	0.006936694	52.49968	33
ATCAGCA	20	0.006936694	52.49968	18
GTAGGCA	20	0.006936694	52.49968	52
TCAGCAC	20	0.006936694	52.49968	19
GGGGTAC	20	0.006936694	52.49968	27
GATTGAT	20	0.006936694	52.49968	64
AGTCGCC	20	0.006936694	52.49968	32
GATCTCG	2690	0.0	50.482964	36
TCTCGTA	2715	0.0	50.40485	38
CCGTCTT	2945	0.0	50.390182	47
GCCGTCT	2945	0.0	50.390182	46
>>END_MODULE