FastQCFastQC Report
Wed 25 May 2016
SRR1779842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252295
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA252.388718E-455.9939779
GCGTACA200.006943290652.50475736
CGATGAC200.00694873752.4943522
GACGACT200.00694873752.4943523
ACGTGTA305.862276E-446.6801534
ATCTCGG1350.046.66164442
CGACGTA305.873748E-446.66164422
TCGGTGG1050.046.66164445
TGGTCGC851.8189894E-1245.29822549
GATCTCG1250.044.79517741
CGACGAA1650.044.5406622
AGATCTC1500.044.3285640
CGCTTAT404.5821107E-543.75396350
AGTCCAT404.5874775E-543.74529321
CGACGAG757.530616E-1041.9954822
CTCGGTG1300.040.3802744
GCGGTCG350.001253347740.00362448
TCGCTTA350.001253347740.00362449
CGGTGGT1050.039.99569746
CGCTATG350.001254570439.99569325
Produced by FastQC (version 0.11.5)