##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779842_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252295
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.01221982203373	34.0	24.0	34.0	12.0	34.0
2	29.703684179234624	34.0	31.0	34.0	12.0	34.0
3	29.3580411819497	34.0	27.0	34.0	11.0	34.0
4	29.62954477892943	34.0	27.0	34.0	12.0	34.0
5	30.245910541231495	34.0	32.0	34.0	11.0	34.0
6	32.76988842426525	37.0	32.0	38.0	12.0	38.0
7	32.61224756733189	37.0	31.0	38.0	12.0	38.0
8	32.11141719019402	37.0	31.0	38.0	11.0	38.0
9	33.05026258942904	37.0	34.0	38.0	12.0	38.0
10-11	31.64585505063517	37.0	28.5	38.0	11.5	38.0
12-13	32.50779246516974	37.0	31.0	38.0	11.0	38.0
14-15	31.313286034205987	37.0	27.0	38.0	11.0	38.0
16-17	31.938698745516163	37.0	29.0	38.0	11.0	38.0
18-19	32.7925900235835	37.5	33.5	38.0	11.0	38.0
20-21	31.520339285360393	37.0	27.5	38.0	11.0	38.0
22-23	32.42096949999009	37.0	32.5	38.0	11.0	38.0
24-25	32.73246199885055	37.5	32.5	38.0	11.0	38.0
26-27	31.632868269287936	37.0	28.5	38.0	11.0	38.0
28-29	32.83023444776948	37.5	32.5	38.0	11.0	38.0
30-31	33.25000693632454	38.0	34.0	38.0	11.0	38.0
32-33	33.33597177906816	38.0	34.0	38.0	11.0	38.0
34-35	33.283380566400446	38.0	34.0	38.0	11.0	38.0
36-37	32.8042252125488	38.0	32.5	38.0	11.0	38.0
38-39	32.74074595215917	38.0	32.5	38.0	11.0	38.0
40-41	32.71788382647298	37.5	32.5	38.0	11.0	38.0
42-43	33.12225569274064	38.0	34.0	38.0	11.0	38.0
44-45	33.18528508293862	38.0	34.0	38.0	11.0	38.0
46-47	33.08648209437365	38.0	34.0	38.0	11.0	38.0
48-49	32.80873382350027	37.5	33.0	38.0	11.0	38.0
50-51	32.18774450544006	37.5	30.5	38.0	11.0	38.0
52-53	32.76601597336452	37.0	33.0	38.0	11.0	38.0
54-55	32.89877127965279	37.5	33.0	38.0	11.0	38.0
56-57	32.96610515468004	38.0	34.0	38.0	11.0	38.0
58-59	33.223510176579005	38.0	34.0	38.0	11.0	38.0
60-61	33.103747597059	38.0	34.0	38.0	11.0	38.0
62-63	33.105539150597515	38.0	34.0	38.0	11.0	38.0
64-65	33.12366079391189	38.0	34.0	38.0	11.0	38.0
66-67	32.96996373293169	38.0	34.0	38.0	11.0	38.0
68-69	32.35152301868844	37.0	31.0	38.0	11.0	38.0
70-71	32.39958976594859	37.0	31.0	38.0	11.0	38.0
72-73	32.55630511900751	37.0	31.5	38.0	11.0	38.0
74-75	32.715059751481405	37.5	32.0	38.0	11.0	38.0
76	31.576888166630333	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	30.0
13	501.0
14	1992.0
15	3732.0
16	5116.0
17	5955.0
18	6221.0
19	5455.0
20	4353.0
21	3451.0
22	2687.0
23	2341.0
24	2284.0
25	2451.0
26	2738.0
27	3013.0
28	3709.0
29	4562.0
30	5437.0
31	6809.0
32	8556.0
33	11365.0
34	15518.0
35	22855.0
36	40420.0
37	80741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76165014487124	23.32724253150107	10.033572076910577	28.877535246717112
2	19.2397788303375	24.940644880001585	31.219009492855587	24.600566796805325
3	17.670980399928656	22.295725242275907	32.75490992687132	27.27838443092412
4	12.795735151310966	21.70276858439525	31.787788105194316	33.71370815909947
5	13.081115360986148	33.82548207455558	36.17709427455954	16.91630828989873
6	26.674488477362996	40.03551524856711	17.305756165621556	15.984240108448347
7	25.887608560363724	28.941370466824427	19.308384764486938	25.86263620832491
8	23.098601598173516	36.15510844748858	19.74806570268899	20.99822425164891
9	22.894245519734575	18.449853928085844	20.067545872923382	38.588354679256206
10-11	22.848111300487535	26.753933964881682	25.057077173094456	25.340877561536328
12-13	22.926976212323297	23.586126530701222	26.063390204701253	27.423507052274225
14-15	24.09198017388478	25.67069702548817	22.94093981810867	27.296382982518374
16-17	24.552308021593856	26.849825996654697	23.636313190167026	24.96155279158442
18-19	21.718984416649985	26.543421519411307	26.028732978316494	25.708861085622214
20-21	23.55635268237579	25.792623714302703	25.606135674508018	25.04488792881349
22-23	24.638657727165526	25.971655465865467	24.374476046272424	25.015210760696583
24-25	21.58302895296456	28.555403833309885	24.1449768816256	25.71659033209996
26-27	23.07911832670744	28.226506482542124	25.11306298628975	23.581312204460687
28-29	21.860277985329763	27.530179820397617	26.609970290887684	23.999571903384936
30-31	22.472437855117157	27.26090842609897	26.00284985542632	24.26380386335756
32-33	21.33752155215125	28.11391426702868	25.903604906954158	24.64495927386591
34-35	21.52957661062566	28.338727354524934	25.784081923059393	24.347614111790016
36-37	23.30446744113677	26.66689130396003	25.53927816109269	24.489363093810514
38-39	23.11291866597694	25.969132829517054	26.924045038242074	23.99390346626393
40-41	21.66211508048182	26.78433408774718	26.766894047793638	24.78665678397736
42-43	20.21851534026838	26.359577238414783	27.45968485117532	25.962222570141524
44-45	19.78452769310655	24.874248363940495	28.981540571501053	26.359683371451904
46-47	20.438231062242604	25.059357002144385	28.61701157808369	25.88540035752932
48-49	20.223587910160546	25.86540729187517	28.48595589677936	25.425048901184926
50-51	22.201236672083713	25.71703198699909	27.02604146022434	25.055689880692857
52-53	18.050790517359275	26.938239159001316	28.107812416384732	26.90315790725468
54-55	18.31666488288481	26.466367784106897	27.785375367161762	27.431591965846525
56-57	18.904101986775967	26.796700334564825	27.13463459495457	27.16456308370463
58-59	18.89150293634387	26.782468748699305	27.171933190496674	27.15409512446015
60-61	18.266369858804826	26.035771558380034	27.54009582162984	28.1577627611853
62-63	19.378174877662538	26.19379364063209	26.681557912345134	27.746473569360237
64-65	19.917739521699918	25.45852783476546	25.901346891445904	28.722385752088726
66-67	19.560559360164913	27.209839347479214	24.669131128531927	28.560470163823947
68-69	20.171744416721	28.13013414931419	23.739612792243953	27.958508641720854
70-71	20.006183250130306	28.164902524628953	24.34436739603915	27.48454682920159
72-73	20.483021320955896	27.543036303891995	24.711737029652735	27.262205345499375
74-75	20.370979633578827	27.909686932669516	24.177296474524134	27.542036959227516
76	21.24380673034999	27.525466724800822	24.153553450394387	27.077173094454793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	4.5
14	9.5
15	8.5
16	19.5
17	34.0
18	35.0
19	56.0
20	95.5
21	115.0
22	161.5
23	306.0
24	500.5
25	597.0
26	733.0
27	965.0
28	1231.0
29	1401.0
30	1734.0
31	2369.0
32	3310.0
33	3949.0
34	4427.0
35	5471.5
36	6713.5
37	7389.0
38	8180.5
39	9925.0
40	11904.0
41	13364.5
42	13799.0
43	14574.5
44	15852.5
45	16408.0
46	16461.0
47	16607.0
48	16167.5
49	15231.5
50	14881.0
51	14045.0
52	12051.5
53	10042.0
54	9190.0
55	8387.0
56	6873.0
57	5563.0
58	4964.0
59	4597.5
60	3783.0
61	3051.0
62	2767.0
63	2519.0
64	2102.5
65	1742.5
66	1409.0
67	1267.0
68	1300.5
69	1192.5
70	991.0
71	931.0
72	898.0
73	801.5
74	729.0
75	720.0
76	664.5
77	568.0
78	513.0
79	499.0
80	459.5
81	375.5
82	284.0
83	237.0
84	223.5
85	209.0
86	171.0
87	134.0
88	114.5
89	82.5
90	59.0
91	40.5
92	33.0
93	23.5
94	16.5
95	16.0
96	13.0
97	12.0
98	9.5
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.927228046532829E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003567252620939773
7	0.006341782437226263
8	0.00277452981628649
9	0.00871995085118611
10-11	0.001981807011633207
12-13	1.981807011633207E-4
14-15	5.945421034899621E-4
16-17	3.963614023266414E-4
18-19	0.002576349115123169
20-21	0.0
22-23	0.002576349115123169
24-25	0.002576349115123169
26-27	7.927228046532829E-4
28-29	0.006143601736062943
30-31	5.945421034899621E-4
32-33	0.0
34-35	0.0037654333221030937
36-37	0.014467191184922411
38-39	0.00812540874769615
40-41	7.927228046532829E-4
42-43	5.945421034899621E-4
44-45	0.004756336827919697
46-47	0.0031708912186131314
48-49	1.981807011633207E-4
50-51	0.001981807011633207
52-53	0.009314492954676073
54-55	0.00871995085118611
56-57	0.010701757862819319
58-59	0.010107215759329357
60-61	0.0219980578291286
62-63	0.00852177015002279
64-65	0.018827166610515467
66-67	0.01684535959888226
68-69	5.945421034899621E-4
70-71	1.981807011633207E-4
72-73	0.0031708912186131314
74-75	0.006738143839552904
76	0.001981807011633207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	252295.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.29539928605347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53776372284594	48.14003586425566
2	8.266003682060918	10.13335992383824
3	5.091886721516809	9.363276891441794
4	3.0870988435776363	7.5689982501042055
5	1.8059177161199405	5.534722374366474
6	1.009426408477154	3.712391685449643
7	0.5673953848326997	2.4345108666469004
8	0.350008786487284	1.7163142657092085
9	0.24118679549822705	1.3305276839328795
>10	1.0373503430877877	9.85960382186256
>50	0.005961595495616921	0.20625837239241634
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.927228046532829E-4	0.0
2	0.0	0.0	0.0	7.927228046532829E-4	0.0
3	0.0	3.963614023266414E-4	0.0	7.927228046532829E-4	0.0
4	0.0	3.963614023266414E-4	0.0	0.0015854456093065657	0.0
5	0.0	3.963614023266414E-4	0.0	0.0015854456093065657	0.0
6	0.0	3.963614023266414E-4	0.0	0.0015854456093065657	0.0
7	0.0	3.963614023266414E-4	0.0	0.0015854456093065657	0.0
8	0.0	3.963614023266414E-4	0.0	0.0019818070116332073	0.0
9	0.0	3.963614023266414E-4	0.0	0.0031708912186131314	0.0
10	0.0	3.963614023266414E-4	0.0	0.0031708912186131314	0.0
11	0.0	3.963614023266414E-4	0.0	0.0031708912186131314	0.0
12	0.0	3.963614023266414E-4	0.0	0.003567252620939773	0.0
13	0.0	3.963614023266414E-4	0.0	0.003567252620939773	0.0
14	0.0	3.963614023266414E-4	0.0	0.003963614023266415	0.0
15	0.0	3.963614023266414E-4	0.0	0.004359975425593056	0.0
16	0.0	3.963614023266414E-4	0.0	0.004756336827919697	0.0
17	0.0	3.963614023266414E-4	0.0	0.004756336827919697	0.0
18	0.0	3.963614023266414E-4	0.0	0.004756336827919697	0.0
19	0.0	3.963614023266414E-4	0.0	0.007134505241879546	0.0
20	0.0	3.963614023266414E-4	0.0	0.013476287679105808	0.0
21	0.0	3.963614023266414E-4	0.0	0.01387264908143245	0.0
22	0.0	3.963614023266414E-4	0.0	0.029727105174498107	0.0
23	0.0	3.963614023266414E-4	0.0	0.029727105174498107	0.0
24	0.0	3.963614023266414E-4	0.0	0.03408708060009116	0.0
25	0.0	3.963614023266414E-4	0.0	0.03686161041637765	0.0
26	0.0	3.963614023266414E-4	0.0	0.041221585841970707	0.0
27	0.0	3.963614023266414E-4	0.0	0.078479557660675	0.0
28	0.0	3.963614023266414E-4	0.0	0.11296299966309281	0.0
29	0.0	3.963614023266414E-4	0.0	0.17281357141441567	0.0
30	0.0	3.963614023266414E-4	0.0	0.24257317822390456	0.0
31	0.0	3.963614023266414E-4	0.0	0.2996492201589409	0.0
32	0.0	3.963614023266414E-4	0.0	0.34760894984046453	0.0
33	0.0	3.963614023266414E-4	0.0	0.3951723181196615	0.0
34	0.0	3.963614023266414E-4	0.0	0.46810281614776356	0.0
35	0.0	3.963614023266414E-4	0.0	0.5893894052597158	0.0
36	0.0	3.963614023266414E-4	0.0	0.8046136467230821	0.0
37	0.0	3.963614023266414E-4	0.0	1.2215858419707089	0.0
38	0.0	3.963614023266414E-4	0.0	1.7911571771140926	0.0
39	0.0	3.963614023266414E-4	0.0	2.4594225014368103	0.0
40	0.0	3.963614023266414E-4	0.0	3.2933668919320636	0.0
41	0.0	3.963614023266414E-4	0.0	4.3548227273628095	0.0
42	0.0	3.963614023266414E-4	0.0	5.462256485463445	0.0
43	0.0	3.963614023266414E-4	0.0	6.589904675082741	0.0
44	0.0	3.963614023266414E-4	0.0	7.6668186052042255	0.0
45	0.0	3.963614023266414E-4	0.0	8.708060009116313	0.0
46	0.0	3.963614023266414E-4	0.0	9.79646841990527	0.0
47	0.0	3.963614023266414E-4	0.0	10.786975564319546	0.0
48	0.0	3.963614023266414E-4	0.0	11.762817336847737	0.0
49	0.0	3.963614023266414E-4	0.0	12.676826730612973	0.0
50	0.0	3.963614023266414E-4	0.0	13.517112903545453	0.0
51	0.0	3.963614023266414E-4	0.0	14.457678511266574	0.0
52	0.0	3.963614023266414E-4	0.0	15.483461820487921	0.0
53	0.0	3.963614023266414E-4	0.0	16.49378703501853	0.0
54	0.0	3.963614023266414E-4	0.0	17.448225291821082	0.0
55	0.0	3.963614023266414E-4	0.0	18.221922749162687	0.0
56	0.0	3.963614023266414E-4	0.0	19.059038030876554	0.0
57	0.0	3.963614023266414E-4	0.0	19.825997344378603	0.0
58	0.0	3.963614023266414E-4	0.0	20.546582373808437	0.0
59	0.0	3.963614023266414E-4	0.0	21.23585485245447	0.0
60	0.0	3.963614023266414E-4	0.0	21.931469113537723	0.0
61	0.0	3.963614023266414E-4	0.0	22.623119760597714	0.0
62	0.0	3.963614023266414E-4	0.0	23.238669018410988	0.0
63	0.0	3.963614023266414E-4	0.0	23.875225430547573	0.0
64	0.0	3.963614023266414E-4	0.0	24.560930656572662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGA	25	2.388718E-4	55.993977	9
GCGTACA	20	0.0069432906	52.504757	36
CGATGAC	20	0.006948737	52.49435	22
GACGACT	20	0.006948737	52.49435	23
ACGTGTA	30	5.862276E-4	46.68015	34
ATCTCGG	135	0.0	46.661644	42
CGACGTA	30	5.873748E-4	46.661644	22
TCGGTGG	105	0.0	46.661644	45
TGGTCGC	85	1.8189894E-12	45.298225	49
GATCTCG	125	0.0	44.795177	41
CGACGAA	165	0.0	44.54066	22
AGATCTC	150	0.0	44.32856	40
CGCTTAT	40	4.5821107E-5	43.753963	50
AGTCCAT	40	4.5874775E-5	43.745293	21
CGACGAG	75	7.530616E-10	41.99548	22
CTCGGTG	130	0.0	40.38027	44
GCGGTCG	35	0.0012533477	40.003624	48
TCGCTTA	35	0.0012533477	40.003624	49
CGGTGGT	105	0.0	39.995697	46
CGCTATG	35	0.0012545704	39.995693	25
>>END_MODULE