FastQCFastQC Report
Wed 25 May 2016
SRR1779842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252295
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2715710.763986602984602No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG35581.41025386947819No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC19970.791533720446303No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6850.27150756059374936Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6820.2703184763867695No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2980.11811569789333914Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT2780.11018846984680632No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT2780.11018846984680632No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCA150.002219142470.0297551
AACTCGT653.6379788E-1253.8370137
TAACTCG653.6379788E-1253.8370136
CTCGTAT39050.052.6927139
TCGTATG39100.052.53582840
CAATACG200.006939556452.51189469
CGTATGC39200.052.4910841
AGCGCCG200.00695044952.49107740
GAGCAAC200.00695044952.49107744
TATGCCG39100.052.17783443
CCGTCTT39200.052.1546747
CGTCTTC38750.052.1279448
CTTGAAA38450.052.0897557
GTATGCC39250.051.97842842
GCCGTCT39250.051.90984746
TCTCGTA38050.051.87029338
TGCCGTC39550.051.76111645
ATCTCGT38400.051.67090637
ATGCCGT39500.051.64945244
TCTTCTG39400.051.63360650