FastQCFastQC Report
Wed 25 May 2016
SRR1779841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62366
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC252.8095837E-670.0000119
CAAGCTG150.002215021570.0000156
TACGTTT150.002215021570.0000133
GTCGGGG150.002215021570.0000117
TGCCGCT301.0084659E-770.0000113
TCGCCGT453.7471182E-1062.22222552
TCGGTGG850.057.6470645
CATCTGC553.45608E-1157.272739
ACGTTTA252.3734802E-456.00000434
CTTCCGA252.3734802E-456.00000418
GCCGTAC252.3734802E-456.00000454
TACGTGT252.3734802E-456.00000433
ATCTCCG200.00692139552.542
CCACTTT200.00692139552.513
AGCGGGA200.00692139552.544
CCGTATC407.369763E-752.555
GTAGATA200.00692139552.538
CGGATCA200.00692139552.556
GGTGGAA200.00692139552.547
ACGTGTA200.00692139552.534
Produced by FastQC (version 0.11.5)