##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779841_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62366
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.50771253567649	23.0	12.0	34.0	12.0	34.0
2	24.548632267581695	24.0	12.0	34.0	12.0	34.0
3	24.760398293942213	27.0	11.0	34.0	11.0	34.0
4	25.156206907609917	27.0	12.0	34.0	12.0	34.0
5	24.850768046692107	31.0	11.0	34.0	11.0	34.0
6	27.16661321874098	31.0	12.0	38.0	11.0	38.0
7	27.46475643780265	33.0	12.0	38.0	11.0	38.0
8	27.273883205592792	31.0	11.0	37.0	11.0	38.0
9	27.62708847769618	34.0	12.0	38.0	11.0	38.0
10-11	26.3510245967354	28.0	11.5	37.0	11.0	38.0
12-13	26.663173844723087	30.5	11.0	38.0	11.0	38.0
14-15	25.746207869672578	27.0	11.0	37.0	11.0	38.0
16-17	26.028797742359618	27.5	11.0	37.0	11.0	38.0
18-19	26.232618734566913	28.0	11.0	38.0	11.0	38.0
20-21	25.87631081037745	28.5	11.0	37.0	11.0	38.0
22-23	26.240555751531282	29.0	11.0	38.0	11.0	38.0
24-25	26.53174005066863	31.0	11.0	38.0	11.0	38.0
26-27	25.60582689285829	26.5	11.0	37.0	11.0	38.0
28-29	26.04593849212712	27.0	11.0	38.0	11.0	38.0
30-31	26.22224449219126	28.0	11.0	38.0	11.0	38.0
32-33	26.469109771349775	31.0	11.0	38.0	11.0	38.0
34-35	26.514398871179807	31.0	11.0	38.0	11.0	38.0
36-37	26.187626270724433	29.0	11.0	38.0	11.0	38.0
38-39	25.98031780136613	27.0	11.0	38.0	11.0	38.0
40-41	26.018904531315137	27.0	11.0	38.0	11.0	38.0
42-43	26.287263893788282	29.0	11.0	38.0	11.0	38.0
44-45	26.38723823878395	31.0	11.0	38.0	11.0	38.0
46-47	26.28962896449989	29.0	11.0	38.0	11.0	38.0
48-49	25.96813969149857	27.0	11.0	38.0	11.0	38.0
50-51	25.58521470031748	26.5	11.0	37.5	11.0	38.0
52-53	26.064402078055352	27.0	11.0	37.5	11.0	38.0
54-55	26.070791777571113	27.0	11.0	38.0	11.0	38.0
56-57	26.14909566109739	29.0	11.0	38.0	11.0	38.0
58-59	26.087251066286118	28.5	11.0	38.0	11.0	38.0
60-61	26.18750601289164	28.0	11.0	38.0	11.0	38.0
62-63	26.072371163775134	27.0	11.0	38.0	11.0	38.0
64-65	26.04933778020075	27.5	11.0	38.0	11.0	38.0
66-67	26.280882211461375	29.0	11.0	38.0	11.0	38.0
68-69	25.893836385209887	27.0	11.0	38.0	11.0	38.0
70-71	25.95881570086265	27.0	11.0	37.0	11.0	38.0
72-73	25.693711317063787	27.0	11.0	37.5	11.0	38.0
74-75	25.92228137125998	27.0	11.0	37.5	11.0	38.0
76	24.92927235993971	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	32.0
13	427.0
14	1697.0
15	3180.0
16	4382.0
17	5063.0
18	5131.0
19	4521.0
20	3394.0
21	2449.0
22	1726.0
23	1098.0
24	860.0
25	646.0
26	630.0
27	587.0
28	586.0
29	695.0
30	731.0
31	867.0
32	1136.0
33	1549.0
34	2015.0
35	2975.0
36	5274.0
37	10713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.74117307507296	19.194753551614664	9.59016130583972	34.47391206747266
2	14.200044896257577	22.5876278741622	28.7592598531251	34.453067376455124
3	13.674117307507297	21.258377962351283	39.65942981752878	25.408074912612644
4	10.335759869159478	38.060802360260396	23.623448673956965	27.979989096623157
5	11.296219093736973	32.37020171247154	36.36115832344547	19.97242087034602
6	17.744495935089716	40.026939050398475	14.268075621762904	27.960489392748904
7	16.492679719696604	37.51703789227241	14.993345199724187	30.996937188306795
8	14.150565220877095	41.38218552072477	13.839493305539966	30.62775595285817
9	19.350234922467568	20.75495902888023	15.719760747903337	44.17504530074886
10-11	15.90074561051872	32.22240038483123	22.370720756834764	29.50613324781528
12-13	15.631914825385627	24.653657441554692	26.456723214572044	33.257704518487635
14-15	24.890364063464578	28.302506995053356	20.18503820221116	26.62209073927091
16-17	26.48018535889234	26.056072668382356	19.317571413682245	28.14617055904306
18-19	18.681953018520005	25.820572436462758	23.653491541730137	31.8439830032871
20-21	24.526985857678863	24.838854504056698	21.21989545585736	29.41426418240708
22-23	26.89772945930344	24.462029375922008	19.485600667051504	29.154640497723044
24-25	18.76548356837624	32.34853161654467	18.502513449157775	30.383471365921316
26-27	22.650525134290067	32.57756754589914	20.992543894812794	23.779363424997996
28-29	19.129437798659442	30.34139379750489	24.38263686219172	26.146531541643952
30-31	21.26335874802575	27.904851239868194	24.46144102107736	26.370348991028692
32-33	16.884199724208703	31.714395664304266	24.166212359298335	27.235192252188696
34-35	16.833564504878655	34.18425842039014	22.910035517570375	26.072141557160837
36-37	22.560858269989417	26.695852977965938	23.575162769813016	27.16812598223163
38-39	23.28001603527761	23.959110042092604	26.688314291441174	26.072559631188614
40-41	19.05365102780361	29.52971170188885	24.047557964275406	27.369079306032134
42-43	14.405400421707515	28.530196983909374	26.290978185054236	30.773424409328875
44-45	14.066032743774354	24.395875759665188	30.38580568606385	31.15228581049661
46-47	16.780110798438237	27.760540683551	27.01814304383032	28.441205474180435
48-49	17.586505467722798	28.376038225956453	25.666228393676043	28.37122791264471
50-51	23.56728240651658	27.500641395676993	21.425822589955743	27.506253607850685
52-53	12.276298909557408	31.362251443232843	24.885343168697883	31.476106478511866
54-55	15.391584618838394	26.300471440941596	24.664058240595235	33.64388569962477
56-57	17.27427980404577	28.78538842074036	22.44894686625562	31.491384908958253
58-59	16.06786347126787	29.348706312489476	23.00494704179729	31.57848317444536
60-61	13.59270009782382	26.65859486505124	24.28195711788572	35.466747919239225
62-63	16.363315640084032	27.380169662759187	22.859571192610527	33.39694350454626
64-65	16.646754600489118	26.493204506274303	22.32610351601652	34.53393737722006
66-67	14.890784873464414	33.58725983898387	18.565769637874073	32.95618564967765
68-69	15.971041913863324	36.078151556938074	17.406920437417824	30.543886091780774
70-71	14.827790783439696	35.96751435076805	19.311804508867013	29.89289035692525
72-73	16.463155531681192	33.226165946426995	21.24800564432721	29.062672877564598
74-75	15.657347198948052	33.579750002004474	18.344144129697483	32.41875866934999
76	18.1560169967129	32.83732862984046	17.795237713461077	31.21141665998557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	3.0
14	8.0
15	12.0
16	16.0
17	19.0
18	31.0
19	43.0
20	60.5
21	78.0
22	105.0
23	138.5
24	167.0
25	189.0
26	252.0
27	347.0
28	453.5
29	528.0
30	571.0
31	650.0
32	824.0
33	962.0
34	1003.0
35	1149.5
36	1439.5
37	1624.0
38	1704.0
39	1955.0
40	2312.0
41	2662.0
42	2826.0
43	3032.5
44	3366.0
45	3458.5
46	3424.0
47	3540.5
48	3696.0
49	3631.0
50	3527.0
51	3400.5
52	3225.0
53	2977.0
54	2778.0
55	2671.5
56	2406.5
57	2063.0
58	1878.0
59	1798.0
60	1543.0
61	1240.5
62	1113.0
63	983.0
64	745.5
65	593.0
66	486.5
67	425.0
68	378.0
69	292.5
70	216.5
71	179.0
72	158.5
73	127.5
74	98.0
75	79.0
76	69.0
77	51.5
78	41.0
79	38.0
80	34.5
81	28.5
82	17.0
83	8.0
84	14.5
85	16.5
86	11.0
87	10.0
88	8.0
89	5.5
90	4.5
91	5.0
92	6.0
93	4.0
94	3.0
95	2.5
96	1.0
97	1.0
98	4.5
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0048103133117403715
7	0.00801718885290062
8	0.0016034377705801239
9	0.00801718885290062
10-11	0.0016034377705801239
12-13	0.0
14-15	8.017188852900619E-4
16-17	8.017188852900619E-4
18-19	0.0016034377705801239
20-21	0.0
22-23	0.0032068755411602478
24-25	0.0024051566558701857
26-27	0.0016034377705801239
28-29	0.0064137510823204956
30-31	8.017188852900619E-4
32-33	0.0
34-35	0.00400859442645031
36-37	0.012827502164640991
38-39	0.005612032197030433
40-41	0.0
42-43	8.017188852900619E-4
44-45	0.0048103133117403715
46-47	8.017188852900619E-4
48-49	0.0
50-51	0.0032068755411602478
52-53	0.009620626623480743
54-55	0.0064137510823204956
56-57	0.007215469967610557
58-59	0.008818907738190681
60-61	0.014430939935221114
62-63	0.011224064394060866
64-65	0.013629221049931051
66-67	0.019241253246961486
68-69	0.0
70-71	0.0
72-73	0.00400859442645031
74-75	0.008818907738190681
76	0.0016034377705801239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	62366.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.51399801173717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.67883433254914	76.63630824487701
2	4.47749867192836	7.5682262771381845
3	2.5290278515595355	6.412147644549915
4	1.2502845867799954	4.226661963249207
5	0.6317826515898916	2.669723888015906
6	0.2675115731957198	1.3565083539107847
7	0.111937466798209	0.6622197992495912
8	0.020869697199666085	0.1411025238110509
9	0.017075206799726798	0.12987845941699003
>10	0.015177961599757152	0.1972228457813552
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0032068755411602478	0.0
2	0.0	0.0	0.0	0.0032068755411602478	0.0
3	0.0	0.0	0.0	0.0032068755411602478	0.0
4	0.0	0.0	0.0	0.0032068755411602478	0.0
5	0.0	0.0	0.0	0.0064137510823204956	0.0
6	0.0	0.0	0.0	0.0064137510823204956	0.0
7	0.0	0.0	0.0	0.0064137510823204956	0.0
8	0.0	0.0	0.0	0.0064137510823204956	0.0
9	0.0	0.0	0.0	0.0064137510823204956	0.0
10	0.0	0.0	0.0	0.0064137510823204956	0.0
11	0.0	0.0	0.0	0.009620626623480743	0.0
12	0.0	0.0	0.0	0.009620626623480743	0.0
13	0.0	0.0	0.0	0.011224064394060866	0.0
14	0.0	0.0	0.0	0.012827502164640991	0.0
15	0.0	0.0	0.0	0.012827502164640991	0.0
16	0.0	0.0	0.0	0.012827502164640991	0.0
17	0.0	0.0	0.0	0.012827502164640991	0.0
18	0.0	0.0	0.0	0.01603437770580124	0.0
19	0.0	0.0	0.0	0.019241253246961486	0.0
20	0.0	0.0	0.0	0.022448128788121732	0.0
21	0.0	0.0	0.0	0.02886187987044223	0.0
22	0.0	0.0	0.0	0.043292819805663345	0.0
23	0.0	0.0	0.0	0.04649969534682359	0.0
24	0.0	0.0	0.0	0.052913446429144084	0.0
25	0.0	0.0	0.0	0.052913446429144084	0.0
26	0.0	0.0	0.0	0.052913446429144084	0.0
27	0.0	0.0	0.0	0.06574094859378507	0.0
28	0.0	0.0	0.0	0.09941314177596768	0.0
29	0.0	0.0	0.0	0.15874033928743225	0.0
30	0.0	0.0	0.0	0.20684347240483597	0.0
31	0.0	0.0	0.0	0.2421191033575987	0.0
32	0.0	0.0	0.0	0.2613603566045602	0.0
33	0.0	0.0	0.0	0.32549786742776515	0.0
34	0.0	0.0	0.0	0.3800147516274893	0.0
35	0.0	0.0	0.0	0.5243241509797005	0.0
36	0.0	0.0	0.0	0.686271365808293	0.0
37	0.0	0.0	0.0	1.0534586152711414	0.0
38	0.0	0.0	0.0	1.6258858993682455	0.0
39	0.0	0.0	0.0	2.2416060032710132	0.0
40	0.0	0.0	0.0	2.8428951672385594	0.0
41	0.0	0.0	0.0	3.7231825032870476	0.0
42	0.0	0.0	0.0	4.366161049289677	0.0
43	0.0	0.0	0.0	5.150242119103358	0.0
44	0.0	0.0	0.0	5.924702562293557	0.0
45	0.0	0.0	0.0	6.954109611005997	0.0
46	0.0	0.0	0.0	7.784690376166501	0.0
47	0.0	0.0	0.0	8.535099252797998	0.0
48	0.0	0.0	0.0	9.429817528781708	0.0
49	0.0	0.0	0.0	10.353397684635858	0.0
50	0.0	0.0	0.0	11.131065003367219	0.0
51	0.0	0.0	0.0	11.892697944392777	0.0
52	0.0	0.0	0.0	12.620658692236153	0.0
53	0.0	0.0	0.0	13.385498508802874	0.0
54	0.0	0.0	0.0	14.025270179264343	0.0
55	0.0	0.0	0.0	14.743610300484239	0.0
56	0.0	0.0	0.0	15.518070743674437	0.0
57	0.0	0.0	0.0	16.23320398935317	0.0
58	0.0	0.0	0.0	16.860148157650002	0.0
59	0.0	0.0	0.0	17.48067857486451	0.0
60	0.0	0.0	0.0	18.15412243850816	0.0
61	0.0	0.0	0.0	18.601481576500017	0.0
62	0.0	0.0	0.0	19.127409165250295	0.0
63	0.0	0.0	0.0	19.597216432030272	0.0
64	0.0	0.0	0.0	20.07824776320431	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCGAC	25	2.8095837E-6	70.00001	19
CAAGCTG	15	0.0022150215	70.00001	56
TACGTTT	15	0.0022150215	70.00001	33
GTCGGGG	15	0.0022150215	70.00001	17
TGCCGCT	30	1.0084659E-7	70.00001	13
TCGCCGT	45	3.7471182E-10	62.222225	52
TCGGTGG	85	0.0	57.64706	45
CATCTGC	55	3.45608E-11	57.27273	9
ACGTTTA	25	2.3734802E-4	56.000004	34
CTTCCGA	25	2.3734802E-4	56.000004	18
GCCGTAC	25	2.3734802E-4	56.000004	54
TACGTGT	25	2.3734802E-4	56.000004	33
ATCTCCG	20	0.006921395	52.5	42
CCACTTT	20	0.006921395	52.5	13
AGCGGGA	20	0.006921395	52.5	44
CCGTATC	40	7.369763E-7	52.5	55
GTAGATA	20	0.006921395	52.5	38
CGGATCA	20	0.006921395	52.5	56
GGTGGAA	20	0.006921395	52.5	47
ACGTGTA	20	0.006921395	52.5	34
>>END_MODULE