FastQCFastQC Report
Wed 25 May 2016
SRR1779841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62366
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2366037.93733765192573No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG30884.951415835551423No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC16682.6745342013276465No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6371.021389859859539No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5830.9348042202482122RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3210.5147035243562198No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC3010.48263476894461727No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT2890.4633935156976558No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1770.28380848539268194RNA PCR Primer, Index 24 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1590.2549466055222397No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT1410.22608472565179746No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1410.22608472565179746No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1010.1619472148285925No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG970.155533463746272No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG970.155533463746272No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG960.1539300259756919No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT880.1411025238110509RNA PCR Primer, Index 24 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC860.13789564826989065No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT670.1074303306288683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG207.841905E-570.0505647
GACAGAA150.002215726569.99438517
TATCGTA301.0090298E-769.99438538
AGTGGTG150.002215726569.99438510
CTCTCCG150.002215726569.99438510
TCTGTTC150.002215726569.9943858
AGACGTT150.002215726569.99438525
ATCGTAT455.456968E-1269.99438539
ATATCGT301.0090298E-769.99438537
CATATCG301.0090298E-769.99438536
CTCGTCT150.002215726569.99438539
GACTACA150.002215726569.99438519
CTATACA501.2732926E-1162.994951
AGCATAT352.931847E-759.9951934
TGCTTGC308.29184E-658.32865555
TCTTTAT800.056.8704381
TTGCAAA252.3744212E-455.9955158
CACCTCG509.567884E-1055.9955136
AAAACTC252.3744212E-455.9955169
CTTTATA900.054.4400752
Produced by FastQC (version 0.11.5)