##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779841_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62366
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.51274733027611	34.0	34.0	34.0	34.0	34.0
2	33.60302087675977	34.0	34.0	34.0	34.0	34.0
3	33.75606901196164	34.0	34.0	34.0	34.0	34.0
4	33.54920950517911	34.0	34.0	34.0	34.0	34.0
5	33.60505724272841	34.0	34.0	34.0	34.0	34.0
6	37.33133438091268	38.0	38.0	38.0	37.0	38.0
7	37.3659526023795	38.0	38.0	38.0	37.0	38.0
8	37.30862970208126	38.0	38.0	38.0	37.0	38.0
9	37.21041913863323	38.0	38.0	38.0	36.0	38.0
10-11	37.136693069941956	38.0	38.0	38.0	36.0	38.0
12-13	37.301558541513	38.0	38.0	38.0	36.5	38.0
14-15	37.33355514222493	38.0	38.0	38.0	37.0	38.0
16-17	37.12781002469295	38.0	38.0	38.0	36.0	38.0
18-19	36.93906936471795	38.0	38.0	38.0	34.5	38.0
20-21	37.03631786550364	38.0	38.0	38.0	35.5	38.0
22-23	37.10594715069108	38.0	38.0	38.0	36.0	38.0
24-25	37.18716127377097	38.0	38.0	38.0	36.0	38.0
26-27	37.02111727543854	38.0	38.0	38.0	35.5	38.0
28-29	36.44905076483981	38.0	37.0	38.0	34.0	38.0
30-31	36.58522271750633	38.0	37.0	38.0	34.5	38.0
32-33	36.774380271301666	38.0	37.0	38.0	34.5	38.0
34-35	36.75480229612289	38.0	37.0	38.0	35.0	38.0
36-37	36.631890773819066	38.0	37.0	38.0	34.5	38.0
38-39	34.69416027963955	37.0	35.0	38.0	28.5	38.0
40-41	36.11085367026905	38.0	36.5	38.0	32.5	38.0
42-43	37.02175063335792	38.0	37.5	38.0	35.5	38.0
44-45	36.766042394894654	38.0	37.0	38.0	35.0	38.0
46-47	36.9152663310137	38.0	37.5	38.0	35.5	38.0
48-49	36.46513324567873	38.0	37.0	38.0	34.0	38.0
50-51	36.51454318057916	38.0	37.0	38.0	34.0	38.0
52-53	36.439943238302924	38.0	37.0	38.0	34.0	38.0
54-55	36.47935573870378	38.0	37.0	38.0	34.0	38.0
56-57	36.32117660263606	38.0	37.0	38.0	34.0	38.0
58-59	36.31958919924318	38.0	37.0	38.0	34.0	38.0
60-61	35.80741108937562	38.0	37.0	38.0	31.5	38.0
62-63	35.771397877048386	38.0	37.0	38.0	31.5	38.0
64-65	36.367812590193374	38.0	37.0	38.0	34.0	38.0
66-67	35.97715101176924	38.0	37.0	38.0	32.5	38.0
68-69	35.79965846775487	38.0	37.0	38.0	31.0	38.0
70-71	32.76849565468364	37.0	29.5	38.0	19.5	38.0
72-73	27.32092005259276	29.0	20.0	38.0	10.5	38.0
74-75	26.444873809447458	26.0	11.0	38.0	11.0	38.0
76	26.440496424333773	27.0	11.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	9.0
24	14.0
25	47.0
26	87.0
27	184.0
28	359.0
29	617.0
30	981.0
31	1442.0
32	2255.0
33	3555.0
34	6388.0
35	14492.0
36	14106.0
37	17829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.193134424125763	10.126724414501123	12.839268527430223	57.840872633942894
2	9.338421575858641	10.640413045569701	62.37853958887855	17.642625789693103
3	9.120354039059745	12.349677709008114	63.95471891735882	14.575249334573325
4	6.505147035243562	50.354359747298204	28.48346855658532	14.657024660872912
5	6.442612962190937	23.83349902190296	62.436263348619434	7.2876246672866625
6	12.94936343520508	63.68694480967194	15.102780361094187	8.260911394028799
7	11.825353558028413	22.90671199050765	14.820575313472084	50.44735913799185
8	10.540999903793734	63.1898791007921	10.868101208992078	15.401019786422088
9	10.242760478465831	16.48334028156367	10.924221530962383	62.34967770900811
10-11	10.86729949010679	38.51297181156399	34.632652406760094	15.987076291569124
12-13	10.895359651091942	13.141775967674697	39.840618285604336	36.12224609562903
14-15	10.61956835455216	11.937594201969022	16.921880511817335	60.52095693166149
16-17	34.55729083154282	34.999037937337654	11.743578231728828	18.700092999390694
18-19	36.00038482506494	18.90613475291024	12.703235737421032	32.39024468460379
20-21	12.216592374049963	13.60837635891351	12.148446268800308	62.026584998236224
22-23	10.722990090754578	37.90847577205529	12.404996312093127	38.963537825097006
24-25	34.66715839940992	36.966543466234796	12.381242533813307	15.985055600541976
26-27	36.608889459000096	39.69390372959625	13.259628643812333	10.437578167591315
28-29	34.04980277715422	18.65840361735561	13.241990828335954	34.04980277715422
30-31	36.54074335375044	13.61879870442228	37.87881217329955	11.961645768527724
32-33	15.694448898438251	37.45390116409582	36.77564698714043	10.076002950325497
34-35	30.923099124522977	39.6818779463169	19.073693999935863	10.321328929224256
36-37	15.205400378411312	36.49344193951833	13.73344450501876	34.5677131770516
38-39	10.054356540422667	18.441137799442004	36.80611230478145	34.69839335535388
40-41	9.959753711958438	12.483564762851554	40.113202706602955	37.44347881858705
42-43	33.881441811243306	12.147644549915018	38.022319853766476	15.948593785075202
44-45	11.740371356187666	35.23474328961293	40.92053362409005	12.104351730109354
46-47	33.62809864349165	13.105698617836643	20.622614886316263	32.64358785235545
48-49	31.883558349100472	11.216848924093256	15.93656800179585	40.96302472501042
50-51	13.244279093635242	11.290270851039946	40.11610192594493	35.349348129379884
52-53	7.925968693867078	11.32842571208622	63.40534385424685	17.340261739799846
54-55	7.582052680547981	11.698322050755557	44.226635439058036	36.492989829638425
56-57	31.844543376248346	11.49320178077247	20.998676452893754	35.663578390085426
58-59	10.137291696739323	12.86387913198287	59.54947152320005	17.449357648077758
60-61	31.693743685147467	34.561288149728156	21.087998973585876	12.656969191538497
62-63	9.294810110341288	64.05808955606878	13.137509622786759	13.509590710803183
64-65	7.304361770365619	66.77437459910199	12.075048107761384	13.846215522771008
66-67	7.55252606255012	66.90216519647153	11.623897353648758	13.921411387329591
68-69	8.247546661535502	66.67388236803285	10.680841511128216	14.397729459303443
70-71	8.84562649839236	62.843076725707	10.996095159440975	17.315201616459664
72-73	10.773449652805619	52.48167807944577	13.190179129849094	23.554693137899513
74-75	12.435756608750731	47.64795022490198	14.412969748478604	25.503323417868685
76	12.371514247686855	48.82859479482369	13.97508057920816	24.8248103782813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.5
15	4.0
16	15.0
17	26.0
18	28.5
19	35.0
20	65.0
21	91.0
22	118.5
23	147.0
24	158.5
25	169.0
26	249.5
27	323.0
28	388.5
29	461.0
30	532.5
31	600.0
32	679.5
33	763.0
34	762.5
35	877.0
36	990.5
37	989.0
38	1118.0
39	1454.5
40	5451.0
41	10767.0
42	12294.0
43	11006.5
44	7831.5
45	4627.0
46	3310.0
47	2705.5
48	1780.0
49	1379.0
50	1299.0
51	1212.0
52	1029.0
53	894.0
54	855.0
55	830.0
56	768.5
57	673.0
58	614.0
59	551.5
60	474.5
61	423.5
62	387.0
63	352.0
64	255.5
65	175.5
66	133.5
67	110.0
68	101.0
69	85.0
70	65.5
71	53.0
72	40.5
73	36.5
74	46.0
75	47.0
76	35.5
77	26.0
78	19.5
79	11.0
80	8.0
81	7.5
82	8.5
83	7.0
84	5.5
85	2.5
86	1.0
87	1.0
88	2.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04168938203508322
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0024051566558701857
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.011224064394060866
52-53	0.022448128788121732
54-55	0.044896257576243465
56-57	0.05371516531443415
58-59	0.027258442099862102
60-61	0.02084469101754161
62-63	0.022448128788121732
64-65	0.009620626623480743
66-67	0.025655004329281982
68-69	0.0032068755411602478
70-71	0.012025783279350928
72-73	0.014430939935221114
74-75	0.008818907738190681
76	0.00801718885290062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	62366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.94461725940416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.640710960643254	8.078119488182663
2	17.66398645789251	6.692749254401437
3	15.116377486246297	8.591219574768303
4	10.266610241218789	7.77988006285476
5	6.305543800253914	5.972805695410961
6	3.8679644519678376	4.3966263669306995
7	1.878967414303851	2.4917422954815125
8	0.7871349978840456	1.1929577013116122
9	0.41472704189589504	0.7071160568258346
>10	0.8717731696995344	2.8525157938620405
>50	0.0761743546339399	1.1111823750120258
>100	0.06771053745239103	2.613603566045602
>500	0.016927634363097757	1.956194080107751
>1k	0.016927634363097757	7.625950036879068
>5k	0.0	0.0
>10k+	0.008463817181548879	37.93733765192573
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	23660	37.93733765192573	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	3088	4.951415835551423	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1668	2.6745342013276465	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	637	1.021389859859539	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	583	0.9348042202482122	RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	321	0.5147035243562198	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	301	0.48263476894461727	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	289	0.4633935156976558	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	177	0.28380848539268194	RNA PCR Primer, Index 24 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	159	0.2549466055222397	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	141	0.22608472565179746	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	141	0.22608472565179746	No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	101	0.1619472148285925	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	97	0.155533463746272	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	97	0.155533463746272	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	96	0.1539300259756919	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	88	0.1411025238110509	RNA PCR Primer, Index 24 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	86	0.13789564826989065	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT	67	0.1074303306288683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0032068755411602478	0.0
2	0.0	0.0	0.0	0.0032068755411602478	0.0
3	0.0	0.0	0.0	0.0032068755411602478	0.0
4	0.0	0.0	0.0	0.0032068755411602478	0.0
5	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
6	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
7	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
8	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
9	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
10	0.0	0.0032068755411602478	0.0	0.0064137510823204956	0.0
11	0.0	0.0032068755411602478	0.0	0.012827502164640991	0.0
12	0.0	0.0032068755411602478	0.0	0.012827502164640991	0.0
13	0.0	0.0032068755411602478	0.0	0.012827502164640991	0.0
14	0.0	0.0032068755411602478	0.0	0.014430939935221114	0.0
15	0.0	0.0032068755411602478	0.0	0.014430939935221114	0.0
16	0.0	0.0032068755411602478	0.0	0.014430939935221114	0.0
17	0.0	0.0032068755411602478	0.0	0.014430939935221114	0.0
18	0.0	0.0032068755411602478	0.0	0.01603437770580124	0.0
19	0.0	0.0032068755411602478	0.0	0.02084469101754161	0.0
20	0.0	0.0032068755411602478	0.0	0.025655004329281982	0.0
21	0.0	0.0032068755411602478	0.0	0.03206875541160248	0.0
22	0.0	0.0032068755411602478	0.0	0.044896257576243465	0.0
23	0.0	0.0032068755411602478	0.0	0.051310008658563964	0.0
24	0.0	0.0032068755411602478	0.0	0.051310008658563964	0.0
25	0.0	0.0032068755411602478	0.0	0.051310008658563964	0.0
26	0.0	0.0032068755411602478	0.0	0.051310008658563964	0.0
27	0.0	0.0032068755411602478	0.0	0.0673443863643652	0.0
28	0.0	0.0032068755411602478	0.0	0.10582689285828817	0.0
29	0.0	0.0032068755411602478	0.0	0.16515409036975276	0.0
30	0.0	0.0032068755411602478	0.0	0.22127441234005707	0.0
31	0.0	0.0032068755411602478	0.0	0.25334316775165955	0.0
32	0.0	0.0032068755411602478	0.0	0.27258442099862107	0.0
33	0.0	0.0032068755411602478	0.0	0.34634255844530676	0.0
34	0.0	0.0032068755411602478	0.0	0.40085944264503093	0.0
35	0.0	0.0032068755411602478	0.0	0.5595997819324632	0.0
36	0.0	0.0032068755411602478	0.0	0.7343744989256967	0.0
37	0.0	0.0032068755411602478	0.0	1.1304236282589872	0.0
38	0.0	0.0032068755411602478	0.0	1.7285059166853733	0.0
39	0.0	0.0032068755411602478	0.0	2.3538466472116215	0.0
40	0.0	0.0032068755411602478	0.0	3.0000320687554116	0.0
41	0.0	0.0032068755411602478	0.0	3.8899400314273804	0.0
42	0.0	0.0032068755411602478	0.0	4.563383895071032	0.0
43	0.0	0.0032068755411602478	0.0	5.397171535772697	0.0
44	0.0	0.0032068755411602478	0.0	6.21332136099798	0.0
45	0.0	0.0032068755411602478	0.0	7.2892281050572425	0.0
46	0.0	0.0032068755411602478	0.0	8.195170445435012	0.0
47	0.0	0.0032068755411602478	0.0	8.982458390789853	0.0
48	0.0	0.0032068755411602478	0.0	9.912452297726325	0.0
49	0.0	0.0032068755411602478	0.0	10.88092871115672	0.0
50	0.0	0.0032068755411602478	0.0	11.693871660840843	0.0
51	0.0	0.0032068755411602478	0.0	12.495590546130904	0.0
52	0.0	0.0032068755411602478	0.0	13.242792547221242	0.0
53	0.0	0.0032068755411602478	0.0	14.012442677099703	0.0
54	0.0	0.0032068755411602478	0.0	14.62976621877305	0.0
55	0.0	0.0032068755411602478	0.0	15.348106339992945	0.0
56	0.0	0.0032068755411602478	0.0	16.128980534265466	0.0
57	0.0	0.0032068755411602478	0.0	16.850527531026522	0.0
58	0.0	0.0032068755411602478	0.0	17.47907513709393	0.0
59	0.0	0.0032068755411602478	0.0	18.099605554308436	0.0
60	0.0	0.0032068755411602478	0.0	18.78427348234615	0.0
61	0.0	0.0032068755411602478	0.0	19.250873873584965	0.0
62	0.0	0.0032068755411602478	0.0	19.797646153352787	0.0
63	0.0	0.0032068755411602478	0.0	20.270660295673924	0.0
64	0.0	0.0032068755411602478	0.0	20.7645191290126	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAG	20	7.841905E-5	70.05056	47
GACAGAA	15	0.0022157265	69.994385	17
TATCGTA	30	1.0090298E-7	69.994385	38
AGTGGTG	15	0.0022157265	69.994385	10
CTCTCCG	15	0.0022157265	69.994385	10
TCTGTTC	15	0.0022157265	69.994385	8
AGACGTT	15	0.0022157265	69.994385	25
ATCGTAT	45	5.456968E-12	69.994385	39
ATATCGT	30	1.0090298E-7	69.994385	37
CATATCG	30	1.0090298E-7	69.994385	36
CTCGTCT	15	0.0022157265	69.994385	39
GACTACA	15	0.0022157265	69.994385	19
CTATACA	50	1.2732926E-11	62.99495	1
AGCATAT	35	2.931847E-7	59.99519	34
TGCTTGC	30	8.29184E-6	58.328655	55
TCTTTAT	80	0.0	56.870438	1
TTGCAAA	25	2.3744212E-4	55.99551	58
CACCTCG	50	9.567884E-10	55.99551	36
AAAACTC	25	2.3744212E-4	55.99551	69
CTTTATA	90	0.0	54.440075	2
>>END_MODULE