FastQCFastQC Report
Wed 25 May 2016
SRR1779840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244255
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT150.002222878269.9993813
TGTGCCG200.00694012952.51029251
TCTCGGT1500.051.32237243
GACGAAT707.2759576E-1249.9995623
GTAGATC2250.049.76714738
TCGGTGG1900.049.7262245
TAGATCT2300.048.67528539
ACGTGTA651.8371793E-1048.4710434
AGATCTC2450.047.13279340
GGCGGTC604.9039954E-946.67581647
CGAATTT305.8705144E-446.66625624
CTCGGTG2000.045.49028444
GATCTCG2000.045.49028441
ATCTCGG1700.045.28444742
GCCGTCT404.578824E-543.7585854
GACGCTT404.5843655E-543.7496214
CGGTGGT1700.043.2437746
CCTGACG901.8189894E-1242.7598911
ACGAATA757.512426E-1041.9996324
CGACGAA3200.041.55362722
Produced by FastQC (version 0.11.5)