##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779840_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244255
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.48048555812573	34.0	23.0	34.0	12.0	34.0
2	29.204638594911057	34.0	24.0	34.0	12.0	34.0
3	28.772397699125914	34.0	23.0	34.0	11.0	34.0
4	29.022406910810425	34.0	24.0	34.0	12.0	34.0
5	29.46315530900084	34.0	31.0	34.0	11.0	34.0
6	32.004585371844996	37.0	28.0	38.0	12.0	38.0
7	32.17836277660641	37.0	31.0	38.0	12.0	38.0
8	31.600876133548955	37.0	27.0	38.0	11.0	38.0
9	32.413539129188756	37.0	31.0	38.0	12.0	38.0
10-11	31.02121962702913	36.5	25.5	38.0	11.0	38.0
12-13	31.812415303678534	37.0	27.5	38.0	11.0	38.0
14-15	30.648576282982948	36.5	25.0	38.0	11.0	38.0
16-17	31.220953511698838	37.0	26.0	38.0	11.0	38.0
18-19	31.998775869480667	37.0	31.0	38.0	11.0	38.0
20-21	30.85029170334282	36.5	25.5	38.0	11.0	38.0
22-23	31.644134204008104	37.0	28.5	38.0	11.0	38.0
24-25	31.959296636711635	37.0	29.0	38.0	11.0	38.0
26-27	30.92335673783546	37.0	25.0	38.0	11.0	38.0
28-29	32.004788028904215	37.0	29.0	38.0	11.0	38.0
30-31	32.37947636691163	38.0	31.0	38.0	11.0	38.0
32-33	32.48581809993654	38.0	31.5	38.0	11.0	38.0
34-35	32.439794477083375	38.0	31.5	38.0	11.0	38.0
36-37	31.987619496018507	37.5	29.0	38.0	11.0	38.0
38-39	31.915025280956378	37.0	29.0	38.0	11.0	38.0
40-41	31.917610693742198	37.0	29.0	38.0	11.0	38.0
42-43	32.278899101349	37.5	31.5	38.0	11.0	38.0
44-45	32.34659884137479	38.0	31.0	38.0	11.0	38.0
46-47	32.252864833882626	38.0	31.0	38.0	11.0	38.0
48-49	31.987422980082293	37.0	29.0	38.0	11.0	38.0
50-51	31.35978997359317	37.0	26.5	38.0	11.0	38.0
52-53	31.911008986509998	37.0	29.0	38.0	11.0	38.0
54-55	32.04252522978035	37.0	29.0	38.0	11.0	38.0
56-57	32.1074307588381	37.0	31.0	38.0	11.0	38.0
58-59	32.314536038156845	38.0	31.0	38.0	11.0	38.0
60-61	32.211023315797014	37.5	31.0	38.0	11.0	38.0
62-63	32.22040490471024	38.0	31.0	38.0	11.0	38.0
64-65	32.21654009129803	37.5	31.0	38.0	11.0	38.0
66-67	32.12838017645494	37.0	31.0	38.0	11.0	38.0
68-69	31.561996683793577	37.0	27.0	38.0	11.0	38.0
70-71	31.598689893758575	37.0	27.0	38.0	11.0	38.0
72-73	31.74263576999447	37.0	27.5	38.0	11.0	38.0
74-75	31.91616548279462	37.0	29.0	38.0	11.0	38.0
76	30.91681644183333	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	94.0
13	866.0
14	2762.0
15	4579.0
16	5923.0
17	7210.0
18	7539.0
19	6838.0
20	5609.0
21	4158.0
22	3220.0
23	2585.0
24	2510.0
25	2480.0
26	2647.0
27	3017.0
28	3528.0
29	4212.0
30	4979.0
31	6087.0
32	7818.0
33	9961.0
34	13714.0
35	20660.0
36	36356.0
37	74900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.255143190518105	20.64727436490553	12.55204601748173	30.545536427094632
2	18.6059650774805	21.654418538003316	35.268060019242185	24.471556365273997
3	17.21684305336636	22.108452232298212	31.765163456224027	28.9095412581114
4	12.366174694479131	25.446357290536533	31.62187058606784	30.5655974289165
5	12.327690323637182	37.54764487932693	33.47771795869071	16.646946838345173
6	26.987213867815225	39.62718373463146	16.52412087664637	16.861481520906942
7	24.477044837596267	29.36123519368482	22.21817333164099	23.94354663707792
8	21.282615014882232	40.25449230504686	17.54506634568538	20.91782633438553
9	22.88861137838557	18.786005281798268	19.481237332896594	38.844146006919566
10-11	22.60291218610478	27.944171840679306	24.376508447304907	25.076407525911005
12-13	22.523592147550715	23.701664244334815	25.67235061718286	28.10239299093161
14-15	24.431174988280617	25.949474623700375	21.94318607512277	27.676164312896233
16-17	24.95240590698988	26.734983807773087	22.496141296115095	25.816468989121933
18-19	21.40283646130163	26.36091186007664	25.615767580491962	26.620484098129772
20-21	24.057235266422385	25.699576262512537	24.17801068555403	26.065177785511047
22-23	24.96489238441099	25.671957715282357	23.49712793092295	25.866021969383702
24-25	21.53619082322403	28.56268449561305	23.233243397052984	26.66788128410994
26-27	23.323057145430575	28.81324359068838	23.88947570971202	23.974223554169022
28-29	21.879778825465777	27.999336722055833	25.551759221917653	24.569125230560736
30-31	22.68519655768176	27.1090749793248	25.26058938645836	24.945139076535085
32-33	20.90847679679024	28.25960573990297	25.231213281202024	25.600704182104767
34-35	21.50500213924105	28.675451543631304	25.100360088517498	24.71918622861015
36-37	23.905058678044668	25.908015101509328	25.145159573161248	25.041766647284756
38-39	23.42760632580992	25.733558523977685	26.172884999232306	24.66595015098009
40-41	21.900357824232152	27.344690363310324	25.7524953531979	25.002456459259626
42-43	20.26845005608915	27.120947865746313	26.4521768323139	26.15842524585063
44-45	20.080246471304722	25.329839609412584	27.850438592001964	26.739475327280733
46-47	20.89884052274737	25.967868723592414	26.837271953096852	26.296018800563363
48-49	21.18261894867853	26.26645568454215	26.71230212749407	25.83862323928525
50-51	23.417809621289663	26.030296827021495	25.1482088024565	25.40368474923234
52-53	18.680200551537197	27.533457671464806	26.611976996763246	27.17436478023474
54-55	19.410232893314554	26.280340004585806	26.375741098627536	27.933686003472108
56-57	19.698108108661426	26.954282192964317	25.8899013825281	27.45770831584615
58-59	19.69216784591195	27.272094470649893	25.76609997379455	27.269637709643607
60-61	18.77979164277875	26.244492129273205	26.224221526970137	28.751494700977904
62-63	19.564429649738056	26.617778623969475	25.664584625652303	28.15320710064017
64-65	20.166825001637946	26.147177815632578	25.529876171132805	28.156121011596674
66-67	19.48260682623206	28.643557666714443	23.631784770991583	28.242050736061913
68-69	19.447338738897336	29.68972936015508	23.39915948324391	27.463772417703662
70-71	19.617652898923048	29.40559948741988	24.01736713141416	26.959380482242906
72-73	20.175359583362333	28.159891254949006	24.507556061431128	27.157193100257533
74-75	20.025959083734424	28.861055437383946	23.399002172128377	27.713983306753253
76	20.490407212400612	28.368120665230954	23.490661054838156	27.650811067530277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	4.5
11	7.0
12	6.0
13	3.5
14	8.5
15	16.5
16	23.5
17	30.0
18	38.0
19	61.0
20	82.0
21	88.0
22	124.0
23	224.0
24	373.5
25	459.0
26	604.5
27	895.5
28	1209.5
29	1378.0
30	1575.0
31	2101.5
32	2873.5
33	3316.0
34	3874.5
35	5093.5
36	6316.0
37	6878.0
38	7785.5
39	9531.5
40	11270.0
41	12739.0
42	13308.0
43	13828.5
44	14811.5
45	15459.5
46	15645.0
47	15743.5
48	15520.0
49	14615.5
50	14033.0
51	13176.0
52	11417.5
53	9872.5
54	9229.0
55	8483.0
56	7193.0
57	6046.5
58	5444.0
59	4958.0
60	4029.5
61	3381.0
62	3175.0
63	2860.0
64	2359.5
65	1968.0
66	1609.5
67	1457.0
68	1450.5
69	1350.5
70	1182.5
71	1108.0
72	1041.5
73	893.0
74	749.5
75	688.0
76	639.5
77	530.0
78	479.5
79	490.0
80	419.5
81	334.0
82	257.5
83	196.0
84	174.0
85	151.0
86	123.0
87	96.0
88	78.5
89	59.0
90	41.0
91	31.0
92	37.0
93	28.0
94	17.5
95	15.5
96	15.0
97	14.0
98	12.5
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0024564492026775296
7	0.00736934760803259
8	0.0016376328017850196
9	0.008188164008925098
10-11	0.002251745102454402
12-13	0.0
14-15	6.141123006693824E-4
16-17	8.188164008925098E-4
18-19	0.0028658574031237847
20-21	0.0
22-23	0.0024564492026775296
24-25	0.0032752656035700393
26-27	4.094082004462549E-4
28-29	0.005527010706024442
30-31	4.094082004462549E-4
32-33	0.0
34-35	0.0038893779042394223
36-37	0.016785736218296453
38-39	0.007164643507809461
40-41	4.094082004462549E-4
42-43	4.094082004462549E-4
44-45	0.0030705615033469118
46-47	0.0028658574031237847
48-49	2.0470410022312746E-4
50-51	0.0020470410022312745
52-53	0.011668133712718265
54-55	0.009416388610263863
56-57	0.011668133712718265
58-59	0.012691654213833902
60-61	0.02415508382632904
62-63	0.009621092710486992
64-65	0.019242185420973983
66-67	0.020470410022312747
68-69	2.0470410022312746E-4
70-71	2.0470410022312746E-4
72-73	0.005731714806247569
74-75	0.010030500910933247
76	0.003684673804016295
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	244255.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.657571044355706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93975444458589	49.9054865997976
2	6.675986125543097	8.232501776536132
3	4.204766282770923	7.777670274145791
4	2.8013260914898432	6.908918500177692
5	1.7806807111993155	5.489622372904282
6	1.1149323414658132	4.1246412032146935
7	0.6433648525236052	2.7767819871340937
8	0.405969625952627	2.002488084321969
9	0.28776513693232375	1.5968609437044152
>10	1.1393472052732843	10.939849748922601
>50	0.006107182263256683	0.24517850914073414
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0016376328017850196	0.0
2	0.0	0.0	0.0	0.0016376328017850196	0.0
3	0.0	0.0	0.0	0.0016376328017850196	0.0
4	0.0	0.0	0.0	0.0024564492026775296	0.0
5	0.0	0.0	0.0	0.004503490204908804	0.0
6	0.0	0.0	0.0	0.004503490204908804	0.0
7	0.0	0.0	0.0	0.004912898405355059	0.0
8	4.094082004462549E-4	0.0	0.0	0.004912898405355059	0.0
9	4.094082004462549E-4	0.0	0.0	0.006959939407586334	0.0
10	4.094082004462549E-4	0.0	0.0	0.008597572209371353	0.0
11	4.094082004462549E-4	0.0	0.0	0.009416388610263863	0.0
12	4.094082004462549E-4	0.0	0.0	0.014329287015618922	0.0
13	4.094082004462549E-4	0.0	0.0	0.015148103416511433	0.0
14	4.094082004462549E-4	0.0	0.0	0.015148103416511433	0.0
15	4.094082004462549E-4	0.0	0.0	0.015557511616957688	0.0
16	4.094082004462549E-4	0.0	0.0	0.016376328017850196	0.0
17	4.094082004462549E-4	0.0	0.0	0.016376328017850196	0.0
18	4.094082004462549E-4	0.0	0.0	0.018013960819635216	0.0
19	4.094082004462549E-4	0.0	0.0	0.02169863462365151	0.0
20	4.094082004462549E-4	0.0	0.0	0.024564492026775298	0.0
21	4.094082004462549E-4	0.0	0.0	0.025383308427667808	0.0
22	4.094082004462549E-4	0.0	0.0	0.03398088063703916	0.0
23	4.094082004462549E-4	0.0	0.0	0.03766555444105545	0.0
24	4.094082004462549E-4	0.0	0.0	0.047491351251765575	0.0
25	4.094082004462549E-4	0.0	0.0	0.04831016765265808	0.0
26	4.094082004462549E-4	0.0	0.0	0.049538392253996845	0.0
27	4.094082004462549E-4	0.0	0.0	0.0536324742584594	0.0
28	4.094082004462549E-4	0.0	0.0	0.06755235307363207	0.0
29	4.094082004462549E-4	0.0	0.0	0.0872039466950523	0.0
30	4.094082004462549E-4	0.0	0.0	0.11299666332316637	0.0
31	4.094082004462549E-4	0.0	0.0	0.15311866696689935	0.0
32	4.094082004462549E-4	0.0	0.0	0.20675114122535873	0.0
33	4.094082004462549E-4	0.0	0.0	0.2427790628646292	0.0
34	4.094082004462549E-4	0.0	0.0	0.30296206833022865	0.0
35	4.094082004462549E-4	0.0	0.0	0.39139423962661973	0.0
36	4.094082004462549E-4	0.0	0.0	0.5662115412171705	0.0
37	4.094082004462549E-4	0.0	0.0	0.8257763403000962	0.0
38	4.094082004462549E-4	0.0	0.0	1.2233117029334097	0.0
39	4.094082004462549E-4	0.0	0.0	1.7010910728541893	0.0
40	4.094082004462549E-4	0.0	0.0	2.2779472272829624	0.0
41	4.094082004462549E-4	0.0	0.0	2.829829481484514	0.0
42	4.094082004462549E-4	0.0	0.0	3.4824261529958447	0.0
43	4.094082004462549E-4	0.0	0.0	4.12028412929111	0.0
44	4.094082004462549E-4	0.0	0.0	4.848621317884997	0.0
45	4.094082004462549E-4	0.0	0.0	5.5937442426971815	0.0
46	4.094082004462549E-4	0.0	0.0	6.36138461853391	0.0
47	4.094082004462549E-4	0.0	0.0	7.132709668174654	0.0
48	4.094082004462549E-4	0.0	0.0	7.81191787271499	0.0
49	4.094082004462549E-4	0.0	0.0	8.450185257210702	0.0
50	4.094082004462549E-4	0.0	4.094082004462549E-4	9.099097254918016	0.0
51	4.094082004462549E-4	0.0	4.094082004462549E-4	9.781990133262369	0.0
52	4.094082004462549E-4	0.0	4.094082004462549E-4	10.462017154203599	0.0
53	4.094082004462549E-4	0.0	4.094082004462549E-4	11.119526724120284	0.0
54	4.094082004462549E-4	0.0	4.094082004462549E-4	11.748377720005731	0.0
55	4.094082004462549E-4	0.0	4.094082004462549E-4	12.445190477165257	0.0
56	4.094082004462549E-4	0.0	4.094082004462549E-4	13.078544963255615	0.0
57	4.094082004462549E-4	0.0	4.094082004462549E-4	13.62551431905181	0.0
58	4.094082004462549E-4	0.0	4.094082004462549E-4	14.06071523612618	0.0
59	4.094082004462549E-4	0.0	4.094082004462549E-4	14.536856973245174	0.0
60	4.094082004462549E-4	0.0	4.094082004462549E-4	15.00194468895212	0.0
61	4.094082004462549E-4	0.0	4.094082004462549E-4	15.55833043335858	0.0
62	4.094082004462549E-4	0.0	4.094082004462549E-4	16.08401056273157	0.0
63	4.094082004462549E-4	0.0	4.094082004462549E-4	16.636711633334016	0.0
64	4.094082004462549E-4	0.0	4.094082004462549E-4	17.186546846533336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCT	15	0.0022228782	69.99938	13
TGTGCCG	20	0.006940129	52.510292	51
TCTCGGT	150	0.0	51.322372	43
GACGAAT	70	7.2759576E-12	49.99956	23
GTAGATC	225	0.0	49.767147	38
TCGGTGG	190	0.0	49.72622	45
TAGATCT	230	0.0	48.675285	39
ACGTGTA	65	1.8371793E-10	48.47104	34
AGATCTC	245	0.0	47.132793	40
GGCGGTC	60	4.9039954E-9	46.675816	47
CGAATTT	30	5.8705144E-4	46.666256	24
CTCGGTG	200	0.0	45.490284	44
GATCTCG	200	0.0	45.490284	41
ATCTCGG	170	0.0	45.284447	42
GCCGTCT	40	4.578824E-5	43.75858	54
GACGCTT	40	4.5843655E-5	43.74962	14
CGGTGGT	170	0.0	43.24377	46
CCTGACG	90	1.8189894E-12	42.75989	11
ACGAATA	75	7.512426E-10	41.99963	24
CGACGAA	320	0.0	41.553627	22
>>END_MODULE