FastQCFastQC Report
Wed 25 May 2016
SRR1779840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244255
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3413013.973101881230681No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG42571.7428507092997072No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC23880.9776667826656568No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT9370.38361548381814087TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC7740.3168819471454013No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3930.1608974227753782TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3530.144521094757528No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT2970.12159423553253772No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2800.11463429612495138No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2790.11422488792450514RNA PCR Primer, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT150.00222535669.9797337
ATCGTAT453.8380676E-1062.2042139
TCTCGTA48000.054.0156138
ATCTCGT48100.053.97605537
GCCGTCT49550.053.84190446
ATGCCGT49650.053.7709344
CCGTCTT49650.053.7334647
CGTATGC49950.053.7281841
TATGCCG49850.053.69558343
AATCTCG47750.053.6389236
TGCCGTC49900.053.5826345
TCGTATG50100.053.49748240
CGTCTTC49800.053.43100448
GTATGCC50050.053.41110642
CTCGTAT49800.053.3279339
TCTTCTG50300.052.96948650
CTGCTTG49850.052.96685854
CTTCTGC50250.052.88284351
TTGAAAT47650.052.87031632
GAAATCT47850.052.79557434