##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779840_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244255
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.551067531882666	34.0	34.0	34.0	34.0	34.0
2	33.61644183332992	34.0	34.0	34.0	34.0	34.0
3	33.75277066999652	34.0	34.0	34.0	34.0	34.0
4	33.56647765654746	34.0	34.0	34.0	34.0	34.0
5	33.59421096804569	34.0	34.0	34.0	34.0	34.0
6	37.28924279953328	38.0	38.0	38.0	37.0	38.0
7	37.351562915805204	38.0	38.0	38.0	37.0	38.0
8	37.25460277169352	38.0	38.0	38.0	36.0	38.0
9	37.169810239299096	38.0	38.0	38.0	36.0	38.0
10-11	37.112333422038446	38.0	38.0	38.0	35.5	38.0
12-13	37.26466602526048	38.0	38.0	38.0	36.5	38.0
14-15	37.28820290270414	38.0	38.0	38.0	36.0	38.0
16-17	37.192313361036625	38.0	38.0	38.0	36.0	38.0
18-19	37.05630181572537	38.0	38.0	38.0	35.5	38.0
20-21	37.08615586170191	38.0	38.0	38.0	36.0	38.0
22-23	37.08100550654029	38.0	38.0	38.0	36.0	38.0
24-25	37.25553008126753	38.0	38.0	38.0	36.0	38.0
26-27	37.18650385867229	38.0	38.0	38.0	36.0	38.0
28-29	36.67199852613048	38.0	37.0	38.0	34.0	38.0
30-31	36.87097705267037	38.0	37.0	38.0	35.5	38.0
32-33	36.941028842807725	38.0	38.0	38.0	36.0	38.0
34-35	36.93292870156189	38.0	38.0	38.0	36.0	38.0
36-37	36.92092280608381	38.0	37.5	38.0	35.5	38.0
38-39	36.40595893635749	38.0	37.0	38.0	34.0	38.0
40-41	36.806589424986186	38.0	37.0	38.0	35.0	38.0
42-43	37.17167509365213	38.0	38.0	38.0	36.0	38.0
44-45	36.94022230865284	38.0	37.5	38.0	35.5	38.0
46-47	37.076809072485716	38.0	38.0	38.0	36.0	38.0
48-49	36.71330167243249	38.0	37.0	38.0	34.5	38.0
50-51	36.873206280321796	38.0	37.5	38.0	35.0	38.0
52-53	36.80572762072424	38.0	37.0	38.0	35.0	38.0
54-55	36.80832122167407	38.0	37.0	38.0	35.0	38.0
56-57	36.67924300423738	38.0	37.0	38.0	34.5	38.0
58-59	36.74692432089415	38.0	37.0	38.0	34.5	38.0
60-61	36.41953491228429	38.0	37.0	38.0	34.0	38.0
62-63	36.26662299645862	38.0	37.0	38.0	34.0	38.0
64-65	36.63839225399685	38.0	37.0	38.0	34.0	38.0
66-67	36.39272072219607	38.0	37.0	38.0	34.0	38.0
68-69	36.4305479928763	38.0	37.0	38.0	34.0	38.0
70-71	35.32612433727048	38.0	36.5	38.0	28.5	38.0
72-73	33.37844875232851	38.0	34.0	38.0	20.0	38.0
74-75	33.07901578268613	38.0	34.0	38.0	11.0	38.0
76	32.90426808048965	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	5.0
23	23.0
24	60.0
25	102.0
26	228.0
27	460.0
28	788.0
29	1404.0
30	2238.0
31	3499.0
32	5783.0
33	9495.0
34	15654.0
35	31142.0
36	41670.0
37	131702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.515739500514066	16.838500641049272	11.750658457959046	38.895101400477614
2	16.869664899387935	16.92821027205175	43.95938670651573	22.242738122044585
3	15.70162330351477	18.911383595013408	41.825960573990294	23.561032527481526
4	11.264866635278704	29.658758264928046	33.63124603385806	25.44512906593519
5	11.362305786984914	31.090049333688153	45.13725409919961	12.410390780127326
6	23.399316288305254	47.35338068821518	16.890954125811138	12.35634889766842
7	22.16331293115801	26.5153221019017	20.106446132116027	31.214918834824264
8	19.492333831446643	45.6956868846083	16.655544410554544	18.156434873390516
9	19.859572987246935	15.925978997359316	17.359317107121655	46.85513090827209
10-11	20.838877402714377	29.88823156127817	29.388344148533296	19.884546887474155
12-13	20.452600765593335	19.163783750588525	30.806329450778897	29.57728603303924
14-15	18.871056887269454	19.630713803197477	20.770506233239853	40.72772307629322
16-17	27.73269738592864	29.925283003418556	20.03152443143436	22.31049517921844
18-19	28.178952324415057	23.34732144684858	21.23702687764836	27.236699351088
20-21	19.235839593867066	21.628625821375202	21.060162535055575	38.07537204970215
22-23	19.2892673640253	30.30828437493603	21.062823688358478	29.33962457268019
24-25	27.569272718340077	30.44376299887002	21.071680068126362	20.915284214663544
26-27	27.60250557818673	31.63312931158011	21.55841231499867	19.205952795234488
28-29	19.124685267445905	23.47157683568402	29.88843626537839	27.515301631491678
30-31	18.722237006407237	29.931628830525476	23.936664551390965	27.409469611676325
32-33	18.3519272891036	22.737303228183663	38.10341651143273	20.807352971280014
34-35	27.714274016908558	29.199606968127572	24.462958793064626	18.623160221899244
36-37	19.58567890114839	39.7813760209617	21.561892284702463	19.071052793187448
38-39	18.241591779083336	24.109025403778837	30.345540521176638	27.30384229596119
40-41	17.87517143968394	20.766616855335613	32.00753311088821	29.350678594092237
42-43	27.1197196361165	20.61030730305338	31.104505965101904	21.165467095728218
44-45	18.578534728050602	29.284763873820392	32.11397924300424	20.022722155124768
46-47	26.642443348140265	20.60735706536202	25.07993695113713	27.670262635360587
48-49	25.92700251786043	20.028658574031237	23.691838447524106	30.352500460584224
50-51	18.709014337688075	20.43043774683529	32.056392586086524	28.804155329390113
52-53	16.63571304249895	20.323118978632785	41.00498602479703	22.03618195407124
54-55	16.12679921682355	20.815617652600622	34.06059786838376	28.996985262192077
56-57	25.049255534847525	20.43623405083253	25.374075818706864	29.140434595613087
58-59	17.209843323483458	20.904878043785875	39.67821006653274	22.207068566197925
60-61	25.078171553540603	29.261040772070796	24.746032038562586	20.91475563582602
62-63	16.707756573019903	39.973175526251126	21.882013268899993	21.43705463182898
64-65	16.211778435206323	41.1014771668697	21.140581653648898	21.546162744275083
66-67	16.358513245921376	40.81631817241711	20.723309012796438	22.10185956886507
68-69	16.364053252317966	41.16511453179027	20.443346224002852	22.02748599188891
70-71	16.88061223236331	39.45729482722616	20.78895754255367	22.87313539785686
72-73	17.25198404573338	35.51523763503386	21.219870760612288	26.012907558620462
74-75	17.702735073183014	34.01776702229375	21.780674114975852	26.498823789547387
76	17.75388281733049	34.48818059348858	21.718389792684377	26.03954679649656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.5
14	6.0
15	7.5
16	23.0
17	38.0
18	44.0
19	60.5
20	122.0
21	173.0
22	182.0
23	244.0
24	418.0
25	539.0
26	622.0
27	861.0
28	1185.0
29	1353.0
30	1600.0
31	2170.0
32	2876.0
33	3259.0
34	3634.5
35	4568.0
36	5669.0
37	6212.0
38	11843.5
39	21030.0
40	23389.0
41	20229.5
42	18266.0
43	16429.5
44	14185.0
45	13036.0
46	12295.0
47	12380.0
48	11885.5
49	11123.0
50	10940.0
51	10244.5
52	8547.5
53	7001.0
54	6456.0
55	6074.0
56	5190.5
57	4165.5
58	3642.0
59	3197.5
60	2667.0
61	2362.5
62	2144.0
63	1995.5
64	1741.0
65	1504.5
66	1308.5
67	1243.0
68	1163.5
69	1021.5
70	955.0
71	951.0
72	924.0
73	784.0
74	697.0
75	723.0
76	630.0
77	491.5
78	423.5
79	401.0
80	346.5
81	288.5
82	237.0
83	189.0
84	161.5
85	113.5
86	73.5
87	54.0
88	49.0
89	35.0
90	19.5
91	10.0
92	7.0
93	4.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05076661685533561
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0012282246013387648
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.094082004462549E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015762215717180814
52-53	0.029682094532353483
54-55	0.04953839225399685
56-57	0.04912898405355059
58-59	0.03664203393993982
60-61	0.03295736013592353
62-63	0.03070561503346912
64-65	0.01535280751673456
66-67	0.0350044011381548
68-69	0.008802276309594481
70-71	0.013919878815172667
72-73	0.02333626742543653
74-75	0.015762215717180814
76	0.01555751161695769
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	244255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.320566620949418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	36.39642180763187	7.395959141061595
2	12.574041987347384	5.110233157970154
3	11.302736027722931	6.890340013510471
4	9.946810653987185	8.084993142412642
5	7.944151186686546	8.071482671797916
6	6.096627311923278	7.433215287302204
7	4.079864608937422	5.803361241325663
8	2.8629568440988034	4.654152422673026
9	1.8031994197525891	3.297783054594584
>10	6.83402506346456	23.0173384372889
>50	0.1168553813917879	1.5782686127203127
>100	0.03223596728049321	1.268756013182944
>500	0.004029495910061651	0.7004974309635422
>1k	0.004029495910061651	2.720517491965364
>5k	0.0	0.0
>10k+	0.0020147479550308256	13.973101881230681
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	34130	13.973101881230681	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	4257	1.7428507092997072	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	2388	0.9776667826656568	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	937	0.38361548381814087	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	774	0.3168819471454013	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	393	0.1608974227753782	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	353	0.144521094757528	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	297	0.12159423553253772	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	280	0.11463429612495138	No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	279	0.11422488792450514	RNA PCR Primer, Index 12 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004094082004462549	0.0
2	0.0	0.0	0.0	0.004094082004462549	0.0
3	0.0	0.0	0.0	0.004094082004462549	0.0
4	0.0	0.0	0.0	0.005731714806247569	0.0
5	0.0	0.0	0.0	0.007778755808478844	0.0
6	0.0	0.0	0.0	0.007778755808478844	0.0
7	0.0	0.0	0.0	0.008188164008925098	0.0
8	0.0	0.0	0.0	0.008188164008925098	0.0
9	0.0	0.0	0.0	0.012282246013387649	0.0
10	0.0	0.0	0.0	0.015966919817403943	0.0
11	0.0	0.0	0.0	0.015966919817403943	0.0
12	0.0	0.0	0.0	0.02169863462365151	0.0
13	0.0	0.0	0.0	0.022926859224990277	0.0
14	0.0	0.0	0.0	0.02333626742543653	0.0
15	0.0	0.0	0.0	0.023745675625882787	0.0
16	0.0	0.0	0.0	0.024564492026775298	0.0
17	0.0	0.0	0.0	0.02497390022722155	0.0
18	0.0	0.0	0.0	0.02743034942989908	0.0
19	0.0	0.0	0.0	0.031933839634807885	0.0
20	0.0	0.0	0.0	0.03561851343882418	0.0
21	0.0	0.0	0.0	0.03602792163927043	0.0
22	0.0	0.0	0.0	0.03971259544328673	0.0
23	0.0	0.0	0.0	0.04298786104685677	0.0
24	0.0	0.0	0.0	0.049128984053550595	0.0
25	0.0	0.0	0.0	0.0499478004544431	0.0
26	0.0	0.0	0.0	0.051176025055781865	0.0
27	0.0	0.0	0.0	0.05649833166158318	0.0
28	0.0	0.0	0.0	0.07164643507809461	0.0
29	0.0	0.0	0.0	0.09170743689996111	0.0
30	0.0	0.0	0.0	0.1199566027307527	0.0
31	0.0	0.0	0.0	0.1617162391762707	0.0
32	0.0	0.0	0.0	0.21657693803606887	0.0
33	0.0	0.0	0.0	0.25628953347935557	0.0
34	0.0	0.0	0.0	0.3242512947534339	0.0
35	0.0	0.0	0.0	0.4151399152525025	0.0
36	0.0	0.0	0.0	0.6042865038586723	0.0
37	0.0	0.0	0.0	0.8925098769728358	0.0
38	0.0	0.0	0.0	1.3051933430226608	0.0
39	0.0	0.0	0.0	1.7940267343554892	0.0
40	0.0	0.0	0.0	2.395856789011484	0.0
41	0.0	0.0	0.0	2.9718941270393646	0.0
42	0.0	0.0	0.0	3.656424638185503	0.0
43	0.0	0.0	0.0	4.329082311518699	0.0
44	0.0	0.0	0.0	5.084849849542486	0.0
45	0.0	0.0	0.0	5.868047736996172	0.0
46	0.0	0.0	0.0	6.691367628093591	0.0
47	0.0	0.0	0.0	7.493398292767804	0.0
48	0.0	0.0	0.0	8.216822582956336	0.0
49	0.0	0.0	0.0	8.891936705492212	0.0
50	0.0	0.0	0.0	9.570326093631655	0.0
51	0.0	0.0	0.0	10.269185891793413	0.0
52	0.0	0.0	0.0	10.965589240752493	0.0
53	0.0	0.0	0.0	11.629649341876318	0.0
54	0.0	0.0	0.0	12.266688501770691	0.0
55	0.0	0.0	0.0	12.961454217927985	0.0
56	0.0	0.0	0.0	13.59603692861968	0.0
57	0.0	0.0	0.0	14.147509774620787	0.0
58	0.0	0.0	0.0	14.582710691695155	0.0
59	0.0	0.0	0.0	15.072772307629322	0.0
60	0.0	0.0	0.0	15.541135288939838	0.0
61	0.0	0.0	0.0	16.113078544963255	0.0
62	0.0	0.0	0.0	16.649403287547848	0.0
63	0.0	0.0	0.0	17.2180712779677	0.0
64	0.0	0.0	0.0	17.7916521667929	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	15	0.002225356	69.97973	37
ATCGTAT	45	3.8380676E-10	62.20421	39
TCTCGTA	4800	0.0	54.01561	38
ATCTCGT	4810	0.0	53.976055	37
GCCGTCT	4955	0.0	53.841904	46
ATGCCGT	4965	0.0	53.77093	44
CCGTCTT	4965	0.0	53.73346	47
CGTATGC	4995	0.0	53.72818	41
TATGCCG	4985	0.0	53.695583	43
AATCTCG	4775	0.0	53.63892	36
TGCCGTC	4990	0.0	53.58263	45
TCGTATG	5010	0.0	53.497482	40
CGTCTTC	4980	0.0	53.431004	48
GTATGCC	5005	0.0	53.411106	42
CTCGTAT	4980	0.0	53.32793	39
TCTTCTG	5030	0.0	52.969486	50
CTGCTTG	4985	0.0	52.966858	54
CTTCTGC	5025	0.0	52.882843	51
TTGAAAT	4765	0.0	52.870316	32
GAAATCT	4785	0.0	52.795574	34
>>END_MODULE