FastQCFastQC Report
Wed 25 May 2016
SRR1779839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176420
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002219917370.0147535
CCTCGGT207.9129044E-569.99489643
TAGATCT2000.054.23066739
GATCTCG1650.053.0264441
AACGTGT200.00693654652.5110634
ATATCCG200.00694433252.49617444
AACGATT200.00694433252.49617422
GTCGGCC200.00695212452.48129720
GTAGATC2050.051.21577538
TCGGTGG1600.050.30883445
TGCGGAC352.0846172E-549.98218519
AGATCTC2250.048.21870840
CTCGGTG1400.047.4965444
TCGGTCG305.8679073E-446.66326545
TCTCGGT1450.045.85872743
ATCTCGG1550.045.15799742
GTGTAGA3050.044.76352736
GCGTAGA551.3019962E-744.5548436
CTGCGGA404.580893E-543.7468118
CGGTGGT1200.043.7468146