##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779839_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	176420
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.48714431470355	32.0	23.0	34.0	12.0	34.0
2	27.873336356422175	34.0	23.0	34.0	12.0	34.0
3	27.487733817027546	34.0	23.0	34.0	11.0	34.0
4	28.03961002153951	34.0	23.0	34.0	12.0	34.0
5	28.304999433170842	34.0	23.0	34.0	11.0	34.0
6	30.42529758530779	37.0	25.0	38.0	11.0	38.0
7	30.64261988436685	37.0	26.0	38.0	12.0	38.0
8	30.013858972905567	37.0	23.0	38.0	11.0	38.0
9	31.05276045799796	37.0	26.0	38.0	11.0	38.0
10-11	29.736707856252124	35.5	23.0	38.0	11.0	38.0
12-13	30.452468540981748	37.0	25.5	38.0	11.0	38.0
14-15	29.363912821675548	35.0	22.0	38.0	11.0	38.0
16-17	29.852689604353248	36.0	23.5	38.0	11.0	38.0
18-19	30.457312096134224	37.0	24.0	38.0	11.0	38.0
20-21	29.518702528058043	35.0	22.5	38.0	11.0	38.0
22-23	30.230291350187052	36.5	24.0	38.0	11.0	38.0
24-25	30.508125495975513	37.0	24.5	38.0	11.0	38.0
26-27	29.507714544836187	36.0	21.5	38.0	11.0	38.0
28-29	30.42435664890602	37.0	24.0	38.0	11.0	38.0
30-31	30.74201054302233	37.0	25.0	38.0	11.0	38.0
32-33	30.8884338510373	37.0	26.0	38.0	11.0	38.0
34-35	30.83890998752976	37.0	26.0	38.0	11.0	38.0
36-37	30.434789139553338	37.0	24.0	38.0	11.0	38.0
38-39	30.31423591429543	37.0	23.5	38.0	11.0	38.0
40-41	30.311566148962704	37.0	23.5	38.0	11.0	38.0
42-43	30.647585307788233	37.0	25.0	38.0	11.0	38.0
44-45	30.74416732796735	37.0	25.5	38.0	11.0	38.0
46-47	30.627621584854325	37.0	25.0	38.0	11.0	38.0
48-49	30.352216302006575	37.0	24.0	38.0	11.0	38.0
50-51	29.835166080943203	36.5	22.0	38.0	11.0	38.0
52-53	30.317189094207006	37.0	23.5	38.0	11.0	38.0
54-55	30.44032706042399	37.0	23.5	38.0	11.0	38.0
56-57	30.477768960435327	37.0	23.5	38.0	11.0	38.0
58-59	30.657570003400977	37.0	24.5	38.0	11.0	38.0
60-61	30.623996712390884	37.0	24.0	38.0	11.0	38.0
62-63	30.53469561274232	37.0	24.0	38.0	11.0	38.0
64-65	30.547970751615463	37.0	24.0	38.0	11.0	38.0
66-67	30.576589955787327	37.0	24.0	38.0	11.0	38.0
68-69	30.043679854891735	37.0	23.0	38.0	11.0	38.0
70-71	30.103933794354383	36.5	23.5	38.0	11.0	38.0
72-73	30.09142670898991	37.0	23.0	38.0	11.0	38.0
74-75	30.32221970298152	37.0	24.0	38.0	11.0	38.0
76	29.262135812266184	35.0	22.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	45.0
13	608.0
14	2716.0
15	5164.0
16	6829.0
17	7851.0
18	7923.0
19	6763.0
20	5329.0
21	3971.0
22	2998.0
23	2345.0
24	1939.0
25	1773.0
26	1872.0
27	1974.0
28	2282.0
29	2596.0
30	3143.0
31	3852.0
32	4884.0
33	6418.0
34	8929.0
35	13266.0
36	23396.0
37	47552.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.0965876884707	20.129237047953747	10.1604126516268	36.61376261194876
2	16.473755809998867	27.577372180024938	31.06280467067226	24.88606733930393
3	14.762498582927105	22.344972225371272	31.418206552545065	31.47432263915656
4	12.058723500736876	25.27547897063825	32.24974492687904	30.416052601745836
5	11.883006461852398	33.83346559347013	36.39836753202585	17.885160412651626
6	22.48190326109754	39.45344163978845	20.634533736175907	17.430121362938106
7	26.582809461994998	27.900187630023073	20.142734863472235	25.374268044509694
8	19.949890030156677	38.08867877468653	21.169761694215815	20.79166950094098
9	22.516496224575405	17.73089045599873	19.903176798712046	39.849436520713816
10-11	20.364250610768803	28.350158998282478	25.396362028602688	25.889228362346035
12-13	20.174016551411405	23.046990137172656	26.762555265842874	30.016438045573064
14-15	24.81379662169822	26.038714431470357	22.358009295998187	26.789479650833236
16-17	26.265311559412535	25.420164494753966	22.016052692737176	26.298471253096324
18-19	21.11668508913641	25.372559022758836	25.021398407164924	28.48935748093983
20-21	24.05311189207573	24.92461172202698	24.005498242829614	27.016778143067675
22-23	26.164219333225635	24.566863076866394	22.64839173206588	26.620525857842097
24-25	20.87379191111867	29.476235014029417	22.237054672221753	27.41291840263016
26-27	23.011126919436116	29.514961540423652	23.492367602129022	23.981543938011214
28-29	21.667039853521796	28.027844463276995	25.666280252935657	24.638835430265548
30-31	22.543426321920197	26.648981546824473	25.91125130725345	24.896340824001882
32-33	19.68200884253486	29.121131390998755	25.270944337376715	25.925915429089674
34-35	20.067001485142903	30.40716723162561	24.594136519777344	24.93169476345415
36-37	23.56184342561044	26.08666073281214	24.511732591034587	25.839763250542834
38-39	23.814436788385716	24.460965910540324	26.933422522795748	24.791174778278208
40-41	21.03441825664048	27.205557256062306	25.962486821072677	25.797537666224535
42-43	18.59800078789476	26.761214038130703	27.088842219822638	27.551942954151894
44-45	17.9720537384502	24.55671447196871	29.56975228161669	27.9014795079644
46-47	19.560245215158446	26.249528813914797	27.730115947204485	26.460110023722272
48-49	19.6686883573291	27.207232740052152	26.698220156444847	26.4258587461739
50-51	23.2777165271759	26.482933755820774	24.449949125785434	25.78940059121789
52-53	16.842358876968213	28.569849345937442	26.670162558993184	27.917629218101165
54-55	18.1028936172625	26.21488559030189	26.418110546439877	29.264110245995735
56-57	19.255941835910374	27.48168199662694	24.864864864864867	28.397511302597827
58-59	18.857303208360616	27.47954205959801	25.243978832019547	28.419175900021827
60-61	17.666085526782044	25.74194554294057	25.792972459639124	30.79899647063826
62-63	18.93505212496386	26.118603425111818	25.251271236883728	29.695073213040597
64-65	19.61085567530487	25.543549127770188	24.469773849546737	30.375821347378206
66-67	18.054980752768028	29.98769764554479	22.62300937133268	29.3343122303545
68-69	18.76147829044326	31.396950459131617	21.714374787439066	28.127196462986053
70-71	18.227963620595343	31.582288705549587	22.917381113658713	27.272366560196353
72-73	19.204312595124524	29.781734391075513	23.73741238624028	27.27654062755968
74-75	18.622824247680754	30.088518150047193	22.555263612889696	28.733393989382357
76	20.19782898279625	29.20386588442026	22.372247257886237	28.226057874897258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	1.0
14	2.5
15	11.5
16	25.0
17	30.0
18	37.0
19	50.5
20	65.0
21	73.0
22	103.5
23	178.0
24	280.5
25	339.0
26	423.5
27	610.0
28	823.0
29	934.0
30	1055.0
31	1386.0
32	1966.5
33	2337.0
34	2669.0
35	3352.5
36	4213.0
37	4722.0
38	5200.5
39	6534.5
40	7878.0
41	8813.0
42	9260.0
43	9761.5
44	10762.5
45	11499.0
46	11736.0
47	11877.5
48	11730.0
49	10890.0
50	10339.0
51	9840.5
52	8722.0
53	7563.5
54	7025.0
55	6563.5
56	5598.5
57	4664.0
58	4233.0
59	3790.0
60	3037.5
61	2440.5
62	2153.0
63	2000.0
64	1723.0
65	1482.5
66	1228.0
67	1090.0
68	980.5
69	819.0
70	683.0
71	599.0
72	574.5
73	484.0
74	386.5
75	355.0
76	318.0
77	262.5
78	249.0
79	254.0
80	196.0
81	132.0
82	113.0
83	100.0
84	97.0
85	77.5
86	50.0
87	39.0
88	30.0
89	22.0
90	17.5
91	13.0
92	14.0
93	11.0
94	8.5
95	11.0
96	13.0
97	9.5
98	7.5
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003967804103843102
7	0.005101462419226845
8	0.004534633261534973
9	0.009069266523069946
10-11	0.001700487473075615
12-13	0.0
14-15	0.0
16-17	5.668291576918716E-4
18-19	0.0028341457884593587
20-21	0.0
22-23	0.001983902051921551
24-25	0.0028341457884593587
26-27	5.668291576918716E-4
28-29	0.006518535313456524
30-31	2.834145788459358E-4
32-33	0.0
34-35	0.00340097494615123
36-37	0.017571703888448024
38-39	0.009352681101915882
40-41	0.0011336583153837433
42-43	2.834145788459358E-4
44-45	0.0056682915769187174
46-47	0.001983902051921551
48-49	0.0
50-51	0.0025507312096134226
52-53	0.012753656048067113
54-55	0.00821902278653214
56-57	0.012753656048067113
58-59	0.01218682689037524
60-61	0.024090239201904545
62-63	0.009636095680761817
64-65	0.018705362203831764
66-67	0.017571703888448024
68-69	0.0
70-71	8.502437365378075E-4
72-73	0.003684389524997166
74-75	0.008785851944224012
76	0.0028341457884593587
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	176420.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.72024340343009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66194335669961	52.0351890748602
2	6.500567707582572	8.284355131752779
3	4.076235708292003	7.79216194506359
4	2.6333730183315374	6.711966788004436
5	1.748499479219771	5.570740620332729
6	1.0081682496196502	3.8544435754424504
7	0.6830499554041772	3.046687659053937
8	0.4188005736011655	2.1348859589889924
9	0.30412844941995226	1.7441224940652418
>10	0.964236359803646	8.792406923483135
>50	9.971420259438754E-4	0.03303982895248523
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00680194989230246	0.0
2	0.0	0.0	0.0	0.00680194989230246	0.0
3	0.0	0.0	0.0	0.00680194989230246	0.0
4	0.0	0.0	0.0	0.02437365378075048	0.0
5	0.0	0.0	0.0	0.02720779956920984	0.0
6	0.0	0.0	0.0	0.02720779956920984	0.0
7	0.0	0.0	0.0	0.02720779956920984	0.0
8	0.0	0.0	0.0	0.02720779956920984	0.0
9	0.0	0.0	0.0	0.030041945357669197	0.0
10	0.0	0.0	0.0	0.0340097494615123	0.0
11	0.0	0.0	0.0	0.0340097494615123	0.0
12	0.0	0.0	0.0	0.03741072440766353	0.0
13	0.0	0.0	0.0	0.0379775535653554	0.0
14	0.0	0.0	0.0	0.03911121188073915	0.0
15	0.0	0.0	0.0	0.04081169935381476	0.0
16	0.0	0.0	0.0	0.04081169935381476	0.0
17	0.0	0.0	0.0	0.04137852851150663	0.0
18	0.0	0.0	0.0	0.046479990930733475	0.0
19	0.0	0.0	0.0	0.04988096587688471	0.0
20	0.0	0.0	0.0	0.05101462419226845	0.0
21	0.0	0.0	0.0	0.05384876998072781	0.0
22	0.0	0.0	0.0	0.0719873030268677	0.0
23	0.0	0.0	0.0	0.07312096134225145	0.0
24	0.0	0.0	0.0	0.08162339870762952	0.0
25	0.0	0.0	0.0	0.08445754449608887	0.0
26	0.0	0.0	0.0	0.08502437365378075	0.0
27	0.0	0.0	0.0	0.09579412764992631	0.0
28	0.0	0.0	0.0	0.11563314816914182	0.0
29	0.0	0.0	0.0	0.1422741185806598	0.0
30	0.0	0.0	0.0	0.1581453349960322	0.0
31	0.0	0.0	0.0	0.17968484298832332	0.0
32	0.0	0.0	0.0	0.2420360503344292	0.0
33	0.0	0.0	0.0	0.2754789706382496	0.0
34	0.0	0.0	0.0	0.3315950572497449	0.0
35	0.0	0.0	0.0	0.42852284321505496	0.0
36	0.0	0.0	0.0	0.6212447568302913	0.0
37	0.0	0.0	0.0	0.9307334769300533	0.0
38	0.0	0.0	0.0	1.333749008048974	0.0
39	0.0	0.0	0.0	1.8348259834485885	0.0
40	0.0	0.0	0.0	2.464573177644258	0.0
41	0.0	0.0	0.0	3.1045232966783813	0.0
42	0.0	0.0	0.0	3.8839133885047046	0.0
43	0.0	0.0	0.0	4.541435211427276	0.0
44	0.0	0.0	0.0	5.208026300872917	0.0
45	0.0	0.0	0.0	5.930166647772362	0.0
46	0.0	0.0	0.0	6.68971771907947	0.0
47	0.0	0.0	0.0	7.419226845028908	0.0
48	0.0	0.0	0.0	8.106223784151457	0.0
49	0.0	0.0	0.0	8.732570003400975	0.0
50	0.0	0.0	0.0	9.384990363904318	0.0
51	0.0	0.0	0.0	10.032309261988436	0.0
52	0.0	0.0	0.0	10.7601179004648	0.0
53	0.0	0.0	0.0	11.384196803083551	0.0
54	0.0	0.0	0.0	11.944790840040811	0.0
55	0.0	0.0	0.0	12.556966330348033	0.0
56	0.0	0.0	0.0	13.116993538147602	0.0
57	0.0	0.0	0.0	13.743906586554813	0.0
58	0.0	0.0	0.0	14.328874277292824	0.0
59	0.0	0.0	0.0	14.856592223103956	0.0
60	0.0	0.0	0.0	15.448928692891963	0.0
61	0.0	0.0	0.0	15.901825189887768	0.0
62	0.0	0.0	0.0	16.406303140233533	0.0
63	0.0	0.0	0.0	16.89944450742546	0.0
64	0.0	0.0	0.0	17.344972225371272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	15	0.0022199173	70.01475	35
CCTCGGT	20	7.9129044E-5	69.994896	43
TAGATCT	200	0.0	54.230667	39
GATCTCG	165	0.0	53.02644	41
AACGTGT	20	0.006936546	52.51106	34
ATATCCG	20	0.006944332	52.496174	44
AACGATT	20	0.006944332	52.496174	22
GTCGGCC	20	0.006952124	52.481297	20
GTAGATC	205	0.0	51.215775	38
TCGGTGG	160	0.0	50.308834	45
TGCGGAC	35	2.0846172E-5	49.982185	19
AGATCTC	225	0.0	48.218708	40
CTCGGTG	140	0.0	47.49654	44
TCGGTCG	30	5.8679073E-4	46.663265	45
TCTCGGT	145	0.0	45.858727	43
ATCTCGG	155	0.0	45.157997	42
GTGTAGA	305	0.0	44.763527	36
GCGTAGA	55	1.3019962E-7	44.55484	36
CTGCGGA	40	4.580893E-5	43.74681	18
CGGTGGT	120	0.0	43.74681	46
>>END_MODULE