FastQCFastQC Report
Wed 25 May 2016
SRR1779839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176420
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3519619.950119034123116TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG42502.4090239201904544TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC24541.3909987529758532RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC9900.561160866114953RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT9310.5277179458111325TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT5320.30155311189207573No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT4600.260741412538261No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4120.23353361296905112TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT3480.19725654687677133No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2690.15247704341911347RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2360.1337716812152817TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAGCTCGTATGCCGT2100.11903412311529304No Hit
CCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2100.11903412311529304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAC150.002223487769.9863924
AACCGGA150.0022259969.966559
GGATCTC401.3460522E-1069.9665535
TAAACCG150.0022259969.966557
CGACACA207.9288E-569.9665525
CGGTAGA150.0022259969.9665514
AACGTGA401.0642907E-861.22073431
CTTTATA1600.059.034282
CGGATCT252.391112E-455.97324834
CACAACG452.696288E-854.4184328
TCTTTAT1750.054.020131
ATCTCGT51050.052.69762837
GATCTCG50850.052.5609136
GAGTCGC200.00691655252.5493956
CGTAGAC200.00691655252.5493950
AACGCTG200.00692432252.53447767
TCTCGTA51350.052.52601238
AAGTACT200.006955467652.47491541
CAATTGC200.006955467652.47491515
GTAGAAC200.006955467652.47491516
Produced by FastQC (version 0.11.5)