##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779838_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119905
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.059455402193404	32.0	21.0	34.0	12.0	34.0
2	27.556390475793336	34.0	21.0	34.0	12.0	34.0
3	27.344756265376756	34.0	23.0	34.0	11.0	34.0
4	27.75362995704933	34.0	23.0	34.0	12.0	34.0
5	27.473408114757515	34.0	21.0	34.0	11.0	34.0
6	30.379250239773153	37.0	25.0	38.0	11.0	38.0
7	30.54780868187315	37.0	26.0	38.0	12.0	38.0
8	29.970184729577582	36.0	23.0	38.0	11.0	38.0
9	30.675317960051707	37.0	26.0	38.0	11.0	38.0
10-11	29.415082773862643	35.0	22.0	38.0	11.0	38.0
12-13	30.070234769192275	36.0	23.5	38.0	11.0	38.0
14-15	29.01734706642759	34.5	21.0	38.0	11.0	38.0
16-17	29.446553521537886	35.5	22.0	38.0	11.0	38.0
18-19	30.062595388015513	37.0	23.0	38.0	11.0	38.0
20-21	29.164947249906177	35.0	22.0	38.0	11.0	38.0
22-23	29.8420207664401	36.5	22.0	38.0	11.0	38.0
24-25	30.129123055752473	36.5	23.0	38.0	11.0	38.0
26-27	29.189062174221256	35.5	21.0	38.0	11.0	38.0
28-29	30.05925107376673	36.5	23.0	38.0	11.0	38.0
30-31	30.341578749843627	37.0	23.0	38.0	11.0	38.0
32-33	30.490842750510822	37.0	24.0	38.0	11.0	38.0
34-35	30.492097910846088	37.0	24.0	38.0	11.0	38.0
36-37	30.067645219131812	37.0	23.0	38.0	11.0	38.0
38-39	29.975013552395644	37.0	22.5	38.0	11.0	38.0
40-41	29.955627371669237	37.0	23.0	38.0	11.0	38.0
42-43	30.27914599057587	37.0	23.5	38.0	11.0	38.0
44-45	30.35858387890413	37.0	23.5	38.0	11.0	38.0
46-47	30.265297527209043	37.0	23.5	38.0	11.0	38.0
48-49	29.97807430882782	37.0	22.5	38.0	11.0	38.0
50-51	29.444243359326133	36.5	21.5	38.0	11.0	38.0
52-53	29.965839623034903	36.5	23.0	38.0	11.0	38.0
54-55	30.076106083983156	37.0	23.0	38.0	11.0	38.0
56-57	30.13971477419624	37.0	23.0	38.0	11.0	38.0
58-59	30.236291230557526	37.0	23.0	38.0	11.0	38.0
60-61	30.21104207497602	37.0	23.0	38.0	11.0	38.0
62-63	30.171047912931073	37.0	23.0	38.0	11.0	38.0
64-65	30.161636295400527	37.0	23.0	38.0	11.0	38.0
66-67	30.14836745757058	37.0	23.0	38.0	11.0	38.0
68-69	29.656415495600683	36.0	22.5	38.0	11.0	38.0
70-71	29.719978316166966	36.0	23.0	38.0	11.0	38.0
72-73	29.897010133021976	36.0	23.0	38.0	11.0	38.0
74-75	29.809123889746047	36.5	23.0	38.0	11.0	38.0
76	29.040748926233267	34.0	22.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	67.0
13	650.0
14	2226.0
15	3647.0
16	4688.0
17	5690.0
18	5691.0
19	4907.0
20	3913.0
21	2685.0
22	1942.0
23	1566.0
24	1359.0
25	1252.0
26	1293.0
27	1426.0
28	1522.0
29	1759.0
30	2176.0
31	2732.0
32	3398.0
33	4398.0
34	5893.0
35	8869.0
36	15554.0
37	30600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.47904591134648	20.7539301947375	15.053584087402527	31.713439806513488
2	20.48621825611943	21.428630999541305	33.634127017221964	24.451023727117303
3	15.024394312163796	27.241566239939953	32.3814686626913	25.352570785204954
4	12.222175889245653	26.18739835703265	28.903715441391103	32.6867103123306
5	17.26783703765481	33.11871898586381	32.10625078186898	17.5071931946124
6	23.419764309483497	43.239952294771605	15.401616306514432	17.93866708923046
7	21.74384466537666	33.62330686595049	18.3333889370788	26.29945953159405
8	19.48957464553795	42.800667222685576	16.08090075062552	21.628857381150958
9	21.22207950356148	20.438887684120974	17.974210552654846	40.3648222596627
10-11	20.76563803169308	30.31776480400334	22.999165971643034	25.91743119266055
12-13	20.51424258472368	24.695903823459503	25.17962211593393	29.61023147588289
14-15	25.157209100613827	26.947808246597276	20.38339004536963	27.511592607419267
16-17	25.868395813352237	26.24369292356449	21.06959676410492	26.818314498978356
18-19	20.955776568461875	26.422718458747735	24.260962031650717	28.360542941139677
20-21	24.53484008173137	25.740794795880074	22.441516200325257	27.2828489220633
22-23	25.913152989353673	25.230503625923163	21.637524447354263	27.218818937368898
24-25	21.12666071741324	29.545549319032883	21.509887158786686	27.817902804767186
26-27	23.791632437752025	29.544175107482268	21.98893276676661	24.675259687999098
28-29	21.59326430272272	28.556654169985446	24.20172062202613	25.648360905265704
30-31	22.153371419040074	27.358325340894872	23.932279721446147	26.556023518618904
32-33	20.180559609691006	29.071765147408364	24.112839331137152	26.634835911763478
34-35	20.30683775297017	30.158756635710443	23.449443496899512	26.084962114419874
36-37	23.62901577742281	26.22939196656838	23.62234279923094	26.51924945677787
38-39	23.36051045728465	25.39900327376608	25.704693788185253	25.53579248076402
40-41	21.364919581833878	27.952245328573987	24.606249139940537	26.076585949651598
42-43	19.074843207899654	27.494912596744065	25.545436349079264	27.88480784627702
44-45	18.38247865921042	25.377086643397178	27.899198912422484	28.341235784969914
46-47	20.04637120314924	26.843171923737717	25.69807009057397	27.412386782539073
48-49	20.557610431635176	27.26461475590991	25.15543620131021	27.022338611144704
50-51	24.299957882797415	26.635586117169225	22.763305491499413	26.301150508533944
52-53	17.406988819057233	28.946589207742	25.118961727895638	28.527460245305132
54-55	18.883323671819937	26.87012840776869	24.368700032946457	29.877847887464913
56-57	19.890233171101965	27.376230810614686	24.051947402004327	28.68158861627902
58-59	18.966869077805953	28.199235953556652	24.1412818630722	28.692613105565197
60-61	18.064308923082056	26.214916433436635	24.821375688741142	30.899398954740164
62-63	18.795327441895378	27.16372720334636	24.193958704328498	29.846986650429763
64-65	19.807972238558207	26.66157818124184	23.73945286269014	29.79099671750981
66-67	18.51651032912216	29.993868843296813	22.07925458481225	29.41036624276878
68-69	18.731990876072206	31.7882147876018	21.022980788877817	28.456813547448178
70-71	18.138184972207046	30.183604451876285	21.685174451334184	29.993036124582478
72-73	17.75278462378909	28.44090258925183	21.96923256560703	31.83708022135205
74-75	17.715648823127093	28.292908736050176	23.38190401521344	30.60953842560929
76	17.396018381831677	29.01310247620954	22.079048548385753	31.51183059357303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	0.5
12	0.0
13	3.5
14	5.0
15	4.5
16	12.5
17	19.0
18	19.5
19	29.5
20	36.5
21	34.0
22	51.0
23	87.5
24	135.5
25	164.0
26	249.0
27	343.0
28	488.0
29	624.0
30	654.5
31	831.0
32	1228.0
33	1479.0
34	1716.5
35	2169.5
36	2637.0
37	2889.0
38	3294.0
39	4196.5
40	5085.0
41	5708.5
42	5941.0
43	6235.5
44	6799.5
45	7309.0
46	7549.0
47	7611.5
48	7654.5
49	7358.5
50	7082.0
51	6726.0
52	6019.0
53	5465.5
54	5263.0
55	4933.5
56	4262.0
57	3567.5
58	3215.0
59	2911.0
60	2423.5
61	2081.0
62	1922.0
63	1729.0
64	1360.5
65	1101.5
66	976.0
67	934.0
68	827.5
69	631.5
70	497.0
71	452.0
72	436.5
73	385.0
74	311.0
75	273.0
76	257.5
77	216.5
78	168.5
79	146.0
80	130.5
81	117.5
82	109.0
83	98.0
84	85.0
85	58.0
86	38.0
87	32.0
88	34.0
89	27.0
90	12.5
91	8.5
92	10.0
93	9.5
94	10.0
95	8.0
96	5.0
97	5.5
98	4.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001667987156498895
7	0.007505942204245028
8	0.004169967891247237
9	0.009173929360743922
10-11	0.004169967891247237
12-13	4.169967891247237E-4
14-15	8.339935782494475E-4
16-17	0.0
18-19	0.0020849839456236184
20-21	0.0
22-23	0.003752971102122514
24-25	0.001667987156498895
26-27	0.0012509903673741712
28-29	0.005420958258621409
30-31	0.0
32-33	0.0
34-35	0.003752971102122514
36-37	0.015428881197614779
38-39	0.010424919728118093
40-41	4.169967891247237E-4
42-43	8.339935782494475E-4
44-45	0.004586964680371961
46-47	0.0025019807347483425
48-49	4.169967891247237E-4
50-51	0.0012509903673741712
52-53	0.011258913306367541
54-55	0.011258913306367541
56-57	0.01209290688461699
58-59	0.014177890830240606
60-61	0.02543680413660815
62-63	0.011258913306367541
64-65	0.02210082982361036
66-67	0.02126683624536091
68-69	4.169967891247237E-4
70-71	4.169967891247237E-4
72-73	0.004586964680371961
74-75	0.009173929360743922
76	0.00333597431299779
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	119905.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.75513948542597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.99500220832655	60.98828239022559
2	6.099630395871591	8.7535965973062
3	3.528673376879983	7.596013510695967
4	2.1618354680490013	6.2049122221758894
5	1.2238778214277413	4.39097618948334
6	0.6892303400822892	2.967349151411534
7	0.400985611009089	2.0140944914724157
8	0.24988958367233083	1.4344689545890497
9	0.1755038471373114	1.1333972728409991
>10	0.47537134754410837	4.5169092197990075
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.339935782494475E-4	0.0
2	0.0	0.0	0.0	8.339935782494475E-4	0.0
3	0.0	0.0	0.0	0.0025019807347483425	0.0
4	0.0	0.0	0.0	0.00333597431299779	0.0
5	0.0	0.0	0.0	0.005003961469496685	0.0
6	0.0	0.0	0.0	0.008339935782494475	0.0
7	0.0	0.0	0.0	0.008339935782494475	0.0
8	0.0	0.0	0.0	0.008339935782494475	0.0
9	0.0	0.0	0.0	0.008339935782494475	0.0
10	0.0	0.0	0.0	0.009173929360743922	0.0
11	0.0	0.0	0.0	0.009173929360743922	0.0
12	0.0	0.0	0.0	0.009173929360743922	0.0
13	0.0	0.0	0.0	0.015011884408490054	0.0
14	0.0	0.0	0.0	0.019181852299737293	0.0
15	0.0	0.0	0.0	0.022517826612735083	0.0
16	0.0	0.0	0.0	0.022517826612735083	0.0
17	0.0	0.0	0.0	0.022517826612735083	0.0
18	0.0	0.0	0.0	0.024185813769233976	0.0
19	0.0	0.0	0.0	0.024185813769233976	0.0
20	0.0	0.0	0.0	0.024185813769233976	0.0
21	0.0	0.0	0.0	0.031691755973479	0.0
22	0.0	0.0	0.0	0.060047537633960216	0.0
23	0.0	0.0	0.0	0.06421750552520746	0.0
24	0.0	0.0	0.0	0.08506734498144364	0.0
25	0.0	0.0	0.0	0.08590133855969309	0.0
26	0.0	0.0	0.0	0.08840331929444144	0.0
27	0.0	0.0	0.0	0.09924523581168425	0.0
28	0.0	0.0	0.0	0.12009507526792043	0.0
29	0.0	0.0	0.0	0.13927692756765772	0.0
30	0.0	0.0	0.0	0.16596472207164004	0.0
31	0.0	0.0	0.0	0.19265251657562238	0.0
32	0.0	0.0	0.0	0.23435219548809474	0.0
33	0.0	0.0	0.0	0.27104791293107044	0.0
34	0.0	0.0	0.0	0.32775947625203283	0.0
35	0.0	0.0	0.0	0.41032484049872814	0.0
36	0.0	0.0	0.0	0.5629456653183771	0.0
37	0.0	0.0	0.0	0.8006338351194696	0.0
38	0.0	0.0	0.0	1.1817689003794671	0.0
39	0.0	0.0	0.0	1.5570660105917185	0.0
40	0.0	0.0	0.0	1.9949126391726784	0.0
41	0.0	0.0	0.0	2.4511071264751263	0.0
42	0.0	0.0	0.0	2.991534965180768	0.0
43	0.0	0.0	0.0	3.557816604812143	0.0
44	0.0	0.0	0.0	4.023185021475335	0.0
45	0.0	0.0	0.0	4.557774905133231	0.0
46	0.0	0.0	0.0	5.130728493390601	0.0
47	0.0	0.0	0.0	5.641966556857512	0.0
48	0.0	0.0	0.0	6.1190108836161965	0.0
49	0.0	0.0	0.0	6.612735081939869	0.0
50	0.0	0.0	0.0	7.055585671990325	0.0
51	0.0	0.0	0.0	7.582669613443977	0.0
52	0.0	0.0	0.0	8.033026145698678	0.0
53	0.0	0.0	0.0	8.540928234852592	0.0
54	0.0	0.0	0.0	9.110545848796963	0.0
55	0.0	0.0	0.0	9.57091030399066	0.0
56	0.0	0.0	0.0	10.055460572953589	0.0
57	0.0	0.0	0.0	10.481631291439056	0.0
58	0.0	0.0	0.0	10.97201951544973	0.0
59	0.0	0.0	0.0	11.404862182561194	0.0
60	0.0	0.0	0.0	11.714273800091739	0.0
61	0.0	0.0	0.0	12.125432634168718	0.0
62	0.0	0.0	0.0	12.569117217797423	0.0
63	0.0	0.0	0.0	12.98111004545265	0.0
64	0.0	0.0	0.0	13.369751052916893	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAGGC	15	0.0022182483	70.01419	3
CGTGTAC	15	0.0022182483	70.01419	35
CCGTGTA	25	2.8220875E-6	70.01418	34
ATCACGG	15	0.0022219229	69.98499	42
CGTACCA	35	2.9492367E-7	60.01216	56
TACGCTG	30	8.345192E-6	58.320824	14
CTAGCTC	25	2.3799921E-4	56.01135	38
TGACGAA	25	2.3849058E-4	55.98799	1
TCTAACG	25	2.3849058E-4	55.98799	11
CTTCCGA	45	2.6720954E-8	54.45548	18
TTAATCG	20	0.006931373	52.51064	57
CACGGTT	20	0.006931373	52.51064	18
TTAGATC	40	7.4173477E-7	52.51064	38
TTTTTGC	20	0.006931373	52.51064	65
ATACTGA	20	0.006931373	52.51064	6
TAGATCC	20	0.006931373	52.51064	39
CCGTTAA	20	0.006931373	52.51064	37
GTTGCCG	20	0.006931373	52.51064	17
TCACGGA	20	0.0069428217	52.488743	43
CGGGTTG	20	0.0069428217	52.488743	48
>>END_MODULE