FastQCFastQC Report
Wed 25 May 2016
SRR1779838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119905
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2606821.740544597806597No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG33272.774696634835912No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC18991.5837538050957007No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT7040.587131479087611RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6630.5529377423793838No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT2900.24185813769233977No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2860.23852216337934198No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT2640.22017430465785412No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2290.19098452941912347No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2080.1734706642758851RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1570.13093699178516324No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1410.1175930945331721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG150.002221975769.984577
GAATCGG207.9083635E-569.9845710
TGTAGTC150.002221975769.984575
CTCGAAA252.3751566E-456.0343957
TCTCGTA35900.055.75372738
ATCTCGT36000.055.69605337
AATCTCG35750.055.6940136
TATGCCG37100.055.45947343
CGTATGC37200.055.40445341
TCGTATG37150.055.3848340
CTCGTAT37050.055.34541739
GTATGCC37200.055.31038742
CGTCAAT36000.055.3072532
GCCGTCT37150.055.29063846
GTCAATC35950.055.2868433
ATGCCGT37100.055.27083244
CCGTCTT37250.055.23614547
CGTCTTC37000.055.23106448
CTTGAAA35950.055.13815357
GCTTGAA36250.055.0682856