##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779838_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119905
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.61067511780159	34.0	34.0	34.0	34.0	34.0
2	33.695967641049165	34.0	34.0	34.0	34.0	34.0
3	33.78549685167424	34.0	34.0	34.0	34.0	34.0
4	33.5985238313665	34.0	34.0	34.0	34.0	34.0
5	33.659380342771364	34.0	34.0	34.0	34.0	34.0
6	37.35855885909678	38.0	38.0	38.0	37.0	38.0
7	37.40578791543305	38.0	38.0	38.0	37.0	38.0
8	37.32853509027981	38.0	38.0	38.0	37.0	38.0
9	37.24580292731746	38.0	38.0	38.0	36.0	38.0
10-11	37.185476001834786	38.0	38.0	38.0	36.5	38.0
12-13	37.336820816479715	38.0	38.0	38.0	36.5	38.0
14-15	37.35543138317835	38.0	38.0	38.0	37.0	38.0
16-17	37.2352070389058	38.0	38.0	38.0	36.0	38.0
18-19	37.08404570284809	38.0	38.0	38.0	36.0	38.0
20-21	37.13666235769985	38.0	38.0	38.0	36.0	38.0
22-23	37.1464034026938	38.0	38.0	38.0	36.0	38.0
24-25	37.30017930861932	38.0	38.0	38.0	36.0	38.0
26-27	37.19077603102456	38.0	38.0	38.0	36.0	38.0
28-29	36.75862557858304	38.0	37.0	38.0	34.5	38.0
30-31	37.05756640673867	38.0	38.0	38.0	36.0	38.0
32-33	37.01751803511113	38.0	38.0	38.0	36.0	38.0
34-35	36.97711104624494	38.0	38.0	38.0	35.5	38.0
36-37	36.873195446395066	38.0	37.5	38.0	35.0	38.0
38-39	35.927496768274885	38.0	37.0	38.0	32.5	38.0
40-41	36.65780409490847	38.0	37.0	38.0	35.0	38.0
42-43	37.17505942204245	38.0	38.0	38.0	36.5	38.0
44-45	36.96733247153997	38.0	38.0	38.0	35.5	38.0
46-47	37.118151870230605	38.0	38.0	38.0	36.0	38.0
48-49	36.73817605604437	38.0	37.0	38.0	34.5	38.0
50-51	36.85282932321421	38.0	37.5	38.0	34.5	38.0
52-53	36.790054626579376	38.0	37.0	38.0	35.0	38.0
54-55	36.79737292022851	38.0	37.0	38.0	35.0	38.0
56-57	36.687219048413326	38.0	37.0	38.0	34.5	38.0
58-59	36.7238230265627	38.0	37.0	38.0	34.5	38.0
60-61	36.359768149785246	38.0	37.0	38.0	34.0	38.0
62-63	36.244243359326134	38.0	37.0	38.0	34.0	38.0
64-65	36.69487093949377	38.0	37.0	38.0	34.5	38.0
66-67	36.381773070347364	38.0	37.0	38.0	34.0	38.0
68-69	36.361573745882154	38.0	37.0	38.0	34.0	38.0
70-71	34.604157457987576	38.0	34.0	38.0	26.5	38.0
72-73	31.481301863975645	38.0	24.0	38.0	11.0	38.0
74-75	30.9825611942788	38.0	23.5	38.0	11.0	38.0
76	30.895292106250782	37.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	12.0
24	18.0
25	58.0
26	110.0
27	233.0
28	399.0
29	730.0
30	1189.0
31	1921.0
32	2912.0
33	4878.0
34	8631.0
35	19317.0
36	22394.0
37	57099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.050693320429176	14.858415792020557	12.208613526005774	44.882277361544496
2	13.717526375046912	14.011092114590717	51.35899253575747	20.912388974604895
3	13.226304157457989	16.865018139360327	49.13056169467495	20.778116008506732
4	9.739377006797048	36.38797381260164	32.42400233518202	21.44864684541929
5	9.683499437054335	29.170593386430927	50.73182936491389	10.414077811600851
6	20.280221842291816	52.46320003335975	16.355448063049916	10.901130061298527
7	19.075101121721364	25.25249155581502	18.35870063800509	37.31370668445853
8	17.0551686752012	51.19219382010759	14.724990617572246	17.02764688711897
9	17.370418247779494	15.260414494808389	15.388015512280557	51.98115174513156
10-11	17.788666027271592	32.76844168299904	30.823985655310455	18.618906634418916
12-13	17.96880864017347	17.158166882115008	33.22338517993411	31.649639297777405
14-15	16.490555022726326	16.948000500396144	19.697677327884573	46.86376714899295
16-17	30.46620241024144	30.63300112589133	17.47800341937367	21.422793044493556
18-19	31.062507818689795	22.042450273132896	17.960051707601853	28.934990200575456
20-21	17.76489721029148	18.517993411450732	17.626871273091197	46.09023810516659
22-23	16.849589258162712	32.10374880113423	18.057628956257037	32.98903298444602
24-25	30.386392447291165	32.1857485988791	17.84844542300507	19.57941353082466
26-27	31.014553187940454	33.64788791126308	18.734414745006465	16.60314415579
28-29	17.36499728952087	21.101288520078395	31.444476877528043	30.08923731287269
30-31	16.901296860014178	17.608106417580586	48.03969809432467	17.450898628080562
32-33	28.278220257704017	18.20357783245069	23.070347358325343	30.447854551519953
34-35	19.227304949751886	18.630165547725284	32.63208373295526	29.51044576956757
36-37	16.30415745798757	44.17080188482549	19.908677703181684	19.616362954005254
38-39	16.154455610691798	22.792210499979152	32.19799007547642	28.855343813852635
40-41	15.980150952837663	17.669821942371044	34.43142487802844	31.918602226762854
42-43	29.663190288938278	17.712846473651947	32.85197803251754	19.771985204892225
44-45	17.148158959176012	31.11296443017389	34.43684583628706	17.302030774363036
46-47	29.34364705391769	18.61223468579292	22.803886410074643	29.240231850214755
48-49	28.653517367916265	17.016804970601726	20.445352570785204	33.884325090696805
50-51	17.800660627596216	17.577949051599017	34.022738268021286	30.598652052783475
52-53	14.93173598741923	17.64769679348937	47.35349203899371	20.067075180097692
54-55	14.358967940052402	17.952569301890886	36.43877568050201	31.2496870775547
56-57	28.4227699266065	17.742117187597792	23.222638076998503	30.61247480879721
58-59	15.954096254364487	18.143743299446434	45.371455746102725	20.530704700086353
60-61	27.998982158574687	30.830583631941867	23.253505087121386	17.91692912236206
62-63	15.211696491886705	47.65277603971134	18.89458974679848	18.24093772160347
64-65	14.299834843100944	49.24636738234656	18.23023539028744	18.22356238426505
66-67	14.416109398400573	49.16211604807456	17.823963022139157	18.59781153138571
68-69	14.259202882534227	49.502270746364516	17.520403354615933	18.71812301648533
70-71	14.652364488670724	46.62332083560975	18.183145172389345	20.541169503330178
72-73	15.655630354446643	40.50318242865604	19.577567006181333	24.263620210715985
74-75	16.223948884134312	37.72839691536437	20.28744333087263	25.760210869628686
76	16.341357623075258	38.22965150226048	20.114108402982833	25.314882471681432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	6.0
17	7.0
18	13.0
19	21.5
20	39.0
21	54.0
22	63.0
23	98.5
24	160.5
25	196.0
26	244.5
27	316.0
28	447.0
29	555.0
30	598.5
31	759.0
32	1068.5
33	1261.0
34	1486.0
35	1942.5
36	2368.5
37	2563.0
38	2885.5
39	7554.0
40	13982.5
41	14992.5
42	13920.0
43	12052.5
44	8733.0
45	6696.0
46	6111.0
47	5750.0
48	5299.0
49	4774.5
50	4340.0
51	4142.5
52	3561.0
53	2977.0
54	2777.0
55	2664.0
56	2378.0
57	1957.5
58	1710.0
59	1587.5
60	1233.0
61	990.0
62	979.0
63	910.5
64	736.5
65	648.5
66	615.5
67	565.0
68	521.0
69	439.5
70	348.0
71	294.0
72	294.0
73	282.5
74	248.5
75	226.0
76	238.0
77	206.5
78	140.0
79	117.0
80	108.0
81	94.5
82	72.5
83	55.0
84	54.5
85	38.5
86	22.5
87	22.0
88	19.5
89	11.5
90	5.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03919769817772403
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.339935782494475E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.169967891247237E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0158458779867395
52-53	0.032942746340853174
54-55	0.055877569742712976
56-57	0.059630540844835496
58-59	0.03794670781034986
60-61	0.03627872065385097
62-63	0.033359743129977894
64-65	0.0158458779867395
66-67	0.04128268212334765
68-69	0.008756932571619198
70-71	0.013760894041115882
72-73	0.02335182019098453
74-75	0.017096868354113674
76	0.0158458779867395
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	119905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.110837746549354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.136994568497286	8.653517367916267
2	15.442063971031986	6.828739418706475
3	13.322269161134582	8.836995955131146
4	10.040736270368136	8.880363621200118
5	7.219372359686179	7.9813185438472125
6	4.72238986119493	6.264959759809849
7	2.957151478575739	4.576956757432968
8	1.957604103802052	3.462741336891706
9	1.2484912492456246	2.484466869605104
>10	3.8586300543150274	12.59080105083191
>50	0.03394689197344598	0.5245819607189024
>100	0.04149064574532287	1.6754930987031398
>500	0.0075437537718768865	1.1400692214669947
>1k	0.0075437537718768865	4.358450439931612
>5k	0.0	0.0
>10k+	0.0037718768859384432	21.740544597806597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	26068	21.740544597806597	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3327	2.774696634835912	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1899	1.5837538050957007	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	704	0.587131479087611	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	663	0.5529377423793838	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	290	0.24185813769233977	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	286	0.23852216337934198	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	264	0.22017430465785412	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	229	0.19098452941912347	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	208	0.1734706642758851	RNA PCR Primer, Index 48 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	157	0.13093699178516324	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	141	0.1175930945331721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004169967891247238	0.0
2	0.0	0.0	0.0	0.004169967891247238	0.0
3	0.0	0.0	0.0	0.00667194862599558	0.0
4	0.0	0.0	0.0	0.007505942204245027	0.0
5	0.0	0.0	0.0	0.010841916517242818	0.0
6	0.0	0.0	0.0	0.0158458779867395	0.0
7	0.0	0.0	0.0	0.0158458779867395	0.0
8	0.0	0.0	0.0	0.0158458779867395	0.0
9	0.0	0.0	0.0	0.0158458779867395	0.0
10	0.0	0.0	0.0	0.01667987156498895	0.0
11	0.0	0.0	0.0	0.01667987156498895	0.0
12	0.0	0.0	0.0	0.01667987156498895	0.0
13	0.0	0.0	0.0	0.024185813769233976	0.0
14	0.0	0.0	0.0	0.025019807347483426	0.0
15	0.0	0.0	0.0	0.028355781660481215	0.0
16	0.0	0.0	0.0	0.028355781660481215	0.0
17	0.0	0.0	0.0	0.028355781660481215	0.0
18	0.0	0.0	0.0	0.02918977523873066	0.0
19	0.0	0.0	0.0	0.02918977523873066	0.0
20	0.0	0.0	0.0	0.02918977523873066	0.0
21	0.0	0.0	0.0	0.03586172386472624	0.0
22	0.0	0.0	0.0	0.04420165964722072	0.0
23	0.0	0.0	0.0	0.045035653225470165	0.0
24	0.0	0.0	0.0	0.04586964680371961	0.0
25	0.0	0.0	0.0	0.04670364038196906	0.0
26	0.0	0.0	0.0	0.049205621116717405	0.0
27	0.0	0.0	0.0	0.063383511946958	0.0
28	0.0	0.0	0.0	0.08756932571619198	0.0
29	0.0	0.0	0.0	0.10925315875067762	0.0
30	0.0	0.0	0.0	0.13927692756765772	0.0
31	0.0	0.0	0.0	0.16763270922813894	0.0
32	0.0	0.0	0.0	0.214336349610108	0.0
33	0.0	0.0	0.0	0.25186606063133316	0.0
34	0.0	0.0	0.0	0.31107960468704393	0.0
35	0.0	0.0	0.0	0.4003169175597348	0.0
36	0.0	0.0	0.0	0.5646136524748759	0.0
37	0.0	0.0	0.0	0.8440015011884409	0.0
38	0.0	0.0	0.0	1.2426504315916767	0.0
39	0.0	0.0	0.0	1.619615528960427	0.0
40	0.0	0.0	0.0	2.063300112589133	0.0
41	0.0	0.0	0.0	2.5361744714565697	0.0
42	0.0	0.0	0.0	3.0941161753054502	0.0
43	0.0	0.0	0.0	3.6837496351278096	0.0
44	0.0	0.0	0.0	4.1682999040907385	0.0
45	0.0	0.0	0.0	4.734581543722113	0.0
46	0.0	0.0	0.0	5.344230849422459	0.0
47	0.0	0.0	0.0	5.870480797297861	0.0
48	0.0	0.0	0.0	6.375880905717026	0.0
49	0.0	0.0	0.0	6.910470789374922	0.0
50	0.0	0.0	0.0	7.3716692381468665	0.0
51	0.0	0.0	0.0	7.943788832825987	0.0
52	0.0	0.0	0.0	8.389975397189442	0.0
53	0.0	0.0	0.0	8.909553396438847	0.0
54	0.0	0.0	0.0	9.484174971852717	0.0
55	0.0	0.0	0.0	9.939535465576915	0.0
56	0.0	0.0	0.0	10.425753721696342	0.0
57	0.0	0.0	0.0	10.864434343855553	0.0
58	0.0	0.0	0.0	11.349818606396731	0.0
59	0.0	0.0	0.0	11.790167215712438	0.0
60	0.0	0.0	0.0	12.10625078186898	0.0
61	0.0	0.0	0.0	12.521579583837205	0.0
62	0.0	0.0	0.0	12.977774071139653	0.0
63	0.0	0.0	0.0	13.408114757516367	0.0
64	0.0	0.0	0.0	13.815937617280348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTG	15	0.0022219757	69.98457	7
GAATCGG	20	7.9083635E-5	69.98457	10
TGTAGTC	15	0.0022219757	69.98457	5
CTCGAAA	25	2.3751566E-4	56.03439	57
TCTCGTA	3590	0.0	55.753727	38
ATCTCGT	3600	0.0	55.696053	37
AATCTCG	3575	0.0	55.69401	36
TATGCCG	3710	0.0	55.459473	43
CGTATGC	3720	0.0	55.404453	41
TCGTATG	3715	0.0	55.38483	40
CTCGTAT	3705	0.0	55.345417	39
GTATGCC	3720	0.0	55.310387	42
CGTCAAT	3600	0.0	55.30725	32
GCCGTCT	3715	0.0	55.290638	46
GTCAATC	3595	0.0	55.28684	33
ATGCCGT	3710	0.0	55.270832	44
CCGTCTT	3725	0.0	55.236145	47
CGTCTTC	3700	0.0	55.231064	48
CTTGAAA	3595	0.0	55.138153	57
GCTTGAA	3625	0.0	55.06828	56
>>END_MODULE