FastQCFastQC Report
Wed 25 May 2016
SRR1779837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586803
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT1100.044.54334322
ACGCTTC901.05501385E-1038.88372815
CTCGTAT556.6957855E-638.18000848
TATATCG501.7090856E-435.001325
ACGAATA1001.2576493E-831.50118824
ACGTTGC450.004309557431.10698115
TGCCGTA450.004309557431.10698119
GTAGATC2200.030.22841338
CGACGAG952.8217983E-729.47228422
TAGATCT2350.028.2989439
CGACGAA3100.028.22446622
CGAACTT500.00720620928.00105725
ACGATAC500.00720620928.00105724
TACTCGA500.00720620928.0010575
GACGATC755.5820397E-528.00105723
AAAGCGT500.00720620928.00105735
GACGACC6800.027.79043270
AGATCTC2300.027.39000540
GACGCTT959.397292E-625.78605314
AGCGTTC700.001225569525.00094237
Produced by FastQC (version 0.11.5)