##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779837_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586803
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.903531508870948	34.0	31.0	34.0	12.0	34.0
2	30.704057409386113	34.0	32.0	34.0	21.0	34.0
3	30.42149068767542	34.0	32.0	34.0	21.0	34.0
4	30.627217311431604	34.0	32.0	34.0	21.0	34.0
5	31.378726761792286	34.0	33.0	34.0	23.0	34.0
6	33.770803830246265	37.0	34.0	38.0	21.0	38.0
7	33.95373234288168	38.0	34.0	38.0	22.0	38.0
8	33.142177187233195	37.0	34.0	38.0	21.0	38.0
9	34.172921747162164	38.0	34.0	38.0	23.0	38.0
10-11	32.73661859261115	37.0	30.5	38.0	17.0	38.0
12-13	33.71691606893625	37.0	34.0	38.0	21.0	38.0
14-15	32.41060202487036	37.0	31.0	38.0	16.0	38.0
16-17	33.150263376294944	37.0	32.5	38.0	17.0	38.0
18-19	34.21230293641989	38.0	34.5	38.0	23.0	38.0
20-21	32.650524963232975	37.0	30.5	38.0	16.5	38.0
22-23	33.693284628742525	38.0	34.0	38.0	17.0	38.0
24-25	34.02162139593697	38.0	34.5	38.0	22.5	38.0
26-27	32.841116183795926	37.0	32.5	38.0	16.0	38.0
28-29	34.26899572769737	38.0	35.0	38.0	24.0	38.0
30-31	34.74944572539677	38.0	36.0	38.0	26.0	38.0
32-33	34.84011073563019	38.0	36.0	38.0	26.0	38.0
34-35	34.711114292190054	38.0	36.0	38.0	25.0	38.0
36-37	34.13542875547671	38.0	34.5	38.0	22.5	38.0
38-39	34.09034377806521	38.0	35.0	38.0	22.5	38.0
40-41	34.070626769120125	38.0	34.5	38.0	22.5	38.0
42-43	34.51173988544708	38.0	35.0	38.0	24.5	38.0
44-45	34.56655640819832	38.0	36.0	38.0	25.0	38.0
46-47	34.46146918130957	38.0	36.0	38.0	24.0	38.0
48-49	34.15101405412038	38.0	35.0	38.0	22.5	38.0
50-51	33.44028404762757	37.5	33.0	38.0	17.0	38.0
52-53	34.044370086724165	37.5	34.0	38.0	23.0	38.0
54-55	34.23185123457106	38.0	35.0	38.0	24.0	38.0
56-57	34.30639243493984	38.0	35.5	38.0	23.5	38.0
58-59	34.63718147316902	38.0	36.0	38.0	25.0	38.0
60-61	34.419095505646695	38.0	35.5	38.0	24.0	38.0
62-63	34.469479535721526	38.0	36.0	38.0	24.0	38.0
64-65	34.47081303265321	38.0	35.5	38.0	24.5	38.0
66-67	34.23510275169009	38.0	35.0	38.0	23.0	38.0
68-69	33.49720945530272	37.0	34.0	38.0	21.0	38.0
70-71	33.48018585453721	37.0	34.0	38.0	21.5	38.0
72-73	33.7336005439645	37.0	34.0	38.0	22.0	38.0
74-75	33.74996975134756	37.5	34.0	38.0	22.0	38.0
76	32.47932270284917	37.0	31.0	38.0	18.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	65.0
13	702.0
14	2201.0
15	3703.0
16	4994.0
17	5996.0
18	6305.0
19	5872.0
20	5156.0
21	4661.0
22	4419.0
23	4507.0
24	5205.0
25	6099.0
26	7242.0
27	8625.0
28	10579.0
29	12760.0
30	15608.0
31	19111.0
32	24366.0
33	31206.0
34	42478.0
35	61899.0
36	105482.0
37	187560.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.52675528289025	18.8159509202454	9.515678254942058	28.141615541922288
2	22.972104777923764	21.216319616634543	28.395219520009267	27.41635608543242
3	21.15019861861647	20.110156219378563	29.567333500339977	29.172311661664992
4	14.874327500029821	19.022568050947253	32.0877705124207	34.01533393660223
5	16.23918078128435	31.48364953826071	33.32004096775238	18.95712871270256
6	29.796878967540515	35.04131780713131	17.664150731177774	17.497652494150408
7	29.309119540265144	28.093487600826894	20.12652252233828	22.47087033656968
8	25.75168076893581	33.604642245456176	18.165086019581278	22.47859096602674
9	25.11150385768409	17.568610875822966	19.37363549444313	37.94624977204981
10-11	24.895383406713357	24.554458145267734	24.20978364325635	26.340374804762558
12-13	25.564989924207886	22.057080533910472	25.155482466417578	27.22244707546406
14-15	24.798760739364162	23.55950010352734	22.752412868746926	28.889326288361566
16-17	25.426273494995318	25.4272959890116	23.298207823612966	25.84822269238012
18-19	23.384365262159598	25.3377062813837	25.272521065901827	26.005407390554875
20-21	24.509503189315666	24.8308205649937	25.378619400377982	25.281056845312648
22-23	25.38552777555288	24.898047522720958	24.1663584943924	25.55006620733376
24-25	23.73277367206133	26.416727805059274	24.091423838604868	25.759074684274523
26-27	24.14346954373761	26.356402218471185	24.585357374439866	24.914770863351343
28-29	23.439381351057712	25.901791610745406	25.655525020770753	25.003302017426133
30-31	23.800467449384502	25.57483237517286	25.161234250423696	25.463465925018937
32-33	23.169419301075404	26.212271100193167	25.338807075305702	25.279502523425723
34-35	23.32251165247919	26.2696728784819	25.416890343141013	24.9909251258979
36-37	24.327606587107535	25.40636774182002	25.06820023811492	25.197825432957526
38-39	24.072866841701156	25.133896272890187	25.873318999934387	24.91991788547427
40-41	23.19068296307405	25.288686357780215	25.958680699936604	25.561949979209132
42-43	22.222212754644698	24.62729934321521	26.86478140688959	26.285706495250494
44-45	21.81555881636152	23.74093975973427	28.138681964050466	26.30481945985374
46-47	21.64778822745473	24.038119876171756	28.17058508249678	26.143506813876737
48-49	21.325153374233132	24.552488070892977	28.37252897068848	25.749829584185413
50-51	22.107161085874917	24.928445379475946	27.29036562515178	25.67402790949736
52-53	19.78387010731503	25.821309783622976	27.775443751432725	26.61937635762927
54-55	19.81651407418517	25.45581748893903	27.652245938631236	27.07542249824456
56-57	19.873433686478233	25.783668071065296	27.28666434872718	27.056233893729292
58-59	20.023894882967056	25.283922169491756	27.354954055184912	27.33722889235628
60-61	20.074353878095096	24.818802905971836	27.604942606715372	27.5019006092177
62-63	20.504440721355174	25.204566333008938	26.76329276378021	27.52770018185568
64-65	21.171011083696612	24.369729278260017	25.960054374459347	28.499205263584027
66-67	21.000408215094414	25.380837411933342	25.060657013246114	28.55809735972613
68-69	21.168832939388103	25.66901102421783	24.63748234063112	28.524673695762942
70-71	21.180299931833673	25.151158827539195	24.716257668711656	28.952283571915473
72-73	21.079607619917585	24.898854421944925	24.195521986168533	29.826015971968957
74-75	20.763326774723247	24.570501933923637	24.97927178124635	29.686899510106763
76	19.72518183739272	24.438297988370586	24.69341567993892	31.14310449429777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.5
11	3.5
12	3.0
13	10.5
14	18.0
15	23.0
16	63.0
17	98.0
18	112.0
19	174.5
20	294.5
21	366.0
22	455.5
23	745.5
24	1155.0
25	1364.0
26	1642.5
27	2224.0
28	3029.5
29	3532.0
30	3991.5
31	5142.0
32	6614.5
33	7396.0
34	8363.0
35	10707.0
36	13361.5
37	14639.0
38	16246.0
39	19699.0
40	22804.0
41	25712.0
42	27361.0
43	28377.5
44	30615.5
45	32170.5
46	32504.0
47	32825.5
48	32699.5
49	31409.5
50	30567.0
51	29782.5
52	27475.0
53	24840.5
54	23729.0
55	22715.5
56	20347.5
57	17842.5
58	16692.0
59	15395.0
60	13189.5
61	11413.5
62	10546.0
63	9884.0
64	8674.5
65	7761.5
66	6826.5
67	6257.0
68	5933.0
69	5221.0
70	4505.5
71	4178.0
72	3922.0
73	3429.5
74	2990.0
75	2787.0
76	2579.5
77	2262.0
78	2003.5
79	1855.0
80	1705.0
81	1378.5
82	1082.5
83	963.0
84	826.0
85	593.0
86	444.5
87	392.0
88	342.0
89	257.5
90	171.0
91	99.5
92	80.0
93	67.0
94	43.5
95	32.5
96	32.0
97	22.0
98	15.0
99	21.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.112448300366563E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001704149433455521
7	0.005112448300366563
8	0.0030674689802199376
9	0.008861577053968709
10-11	0.0029822615085471614
12-13	8.520747167277603E-5
14-15	2.5562241501832813E-4
16-17	4.260373583638802E-4
18-19	0.0023006017351649534
20-21	0.0
22-23	0.001704149433455521
24-25	0.003323091395238266
26-27	4.260373583638802E-4
28-29	0.006901805205494859
30-31	2.5562241501832813E-4
32-33	2.5562241501832813E-4
34-35	0.0030674689802199376
36-37	0.01900126618302906
38-39	0.010480519015751453
40-41	0.0011929046034188646
42-43	8.520747167277604E-4
44-45	0.006134937960439875
46-47	0.003834336225274922
48-49	5.112448300366563E-4
50-51	0.0023006017351649534
52-53	0.012355083392552525
54-55	0.009372821884005364
56-57	0.012781120750916407
58-59	0.011332593732479215
60-61	0.025391826558487263
62-63	0.012610705807570856
64-65	0.02223915010659455
66-67	0.017467531692919088
68-69	6.816597733822083E-4
70-71	5.112448300366563E-4
72-73	0.004260373583638802
74-75	0.007242635092185964
76	0.0025562241501832813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	586803.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.485152535409966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.111554863772	43.9291074337952
2	9.907529156364534	11.588867079180028
3	6.120591263426334	10.738911409451601
4	3.636820566586381	8.508000223228825
5	1.9858898346475224	5.80725349489402
6	1.0860477782006304	3.811060198128418
7	0.557870441394735	2.283899650197729
8	0.3287293818234566	1.5380630431052706
9	0.23173998090468556	1.2198013318587175
>10	1.0179084013866464	10.021131965735828
>50	0.01531833149308617	0.5539041704243172
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007839087393895395	0.0
2	0.0	0.0	0.0	0.008861577053968709	0.0
3	0.0	0.0	0.0	0.009372821884005365	0.0
4	0.0	0.0	0.0	0.030845104745544926	0.0
5	0.0	0.0	0.0	0.0366392128192937	0.0
6	0.0	0.0	0.0	0.038343362252749216	0.0
7	0.0	0.0	1.704149433455521E-4	0.03936585191282253	0.0
8	0.0	0.0	1.704149433455521E-4	0.040729171459586946	0.0
9	0.0	0.0	1.704149433455521E-4	0.04379664043980688	0.0
10	0.0	0.0	1.704149433455521E-4	0.04720493930671793	0.0
11	0.0	0.0	1.704149433455521E-4	0.04737535425006348	0.0
12	0.0	0.0	1.704149433455521E-4	0.04754576919340903	0.0
13	0.0	0.0	1.704149433455521E-4	0.04771618413675458	0.0
14	0.0	0.0	1.704149433455521E-4	0.05061323817362897	0.0
15	0.0	0.0	1.704149433455521E-4	0.05129489794701118	0.0
16	0.0	0.0	1.704149433455521E-4	0.05146531289035673	0.0
17	0.0	0.0	1.704149433455521E-4	0.05197655772039338	0.0
18	0.0	0.0	1.704149433455521E-4	0.05351029221050335	0.0
19	0.0	0.0	1.704149433455521E-4	0.05504402670061332	0.0
20	0.0	0.0	1.704149433455521E-4	0.056577761190723296	0.0
21	0.0	0.0	1.704149433455521E-4	0.05879315545421547	0.0
22	0.0	0.0	1.704149433455521E-4	0.0647576784713098	6.816597733822084E-4
23	0.0	0.0	1.704149433455521E-4	0.06595058307472866	6.816597733822084E-4
24	0.0	0.0	1.704149433455521E-4	0.06850680722491194	6.816597733822084E-4
25	0.0	0.0	1.704149433455521E-4	0.07123344631844077	6.816597733822084E-4
26	0.0	0.0	1.704149433455521E-4	0.07822045899560841	6.816597733822084E-4
27	0.0	0.0	1.704149433455521E-4	0.15610008810452572	6.816597733822084E-4
28	0.0	0.0	1.704149433455521E-4	0.21744946770892445	6.816597733822084E-4
29	0.0	0.0	1.704149433455521E-4	0.2626094276954958	6.816597733822084E-4
30	0.0	0.0	1.704149433455521E-4	0.31560847507596246	6.816597733822084E-4
31	0.0	0.0	1.704149433455521E-4	0.3653696385328637	6.816597733822084E-4
32	0.0	0.0	1.704149433455521E-4	0.4357510101345767	6.816597733822084E-4
33	0.0	0.0	1.704149433455521E-4	0.5063027966796353	6.816597733822084E-4
34	0.0	0.0	1.704149433455521E-4	0.5949185672193223	6.816597733822084E-4
35	0.0	0.0	1.704149433455521E-4	0.7402825138930783	6.816597733822084E-4
36	0.0	0.0	1.704149433455521E-4	1.0238529796200768	6.816597733822084E-4
37	0.0	0.0	1.704149433455521E-4	1.601389222618153	6.816597733822084E-4
38	0.0	1.704149433455521E-4	1.704149433455521E-4	2.249306837217942	6.816597733822084E-4
39	0.0	1.704149433455521E-4	1.704149433455521E-4	2.9824319234905072	6.816597733822084E-4
40	0.0	1.704149433455521E-4	1.704149433455521E-4	3.799401161889084	6.816597733822084E-4
41	0.0	1.704149433455521E-4	1.704149433455521E-4	4.873185719909407	6.816597733822084E-4
42	0.0	1.704149433455521E-4	1.704149433455521E-4	6.165612650242075	6.816597733822084E-4
43	0.0	1.704149433455521E-4	1.704149433455521E-4	7.409300906777913	6.816597733822084E-4
44	0.0	1.704149433455521E-4	1.704149433455521E-4	8.587208995182369	6.816597733822084E-4
45	0.0	1.704149433455521E-4	1.704149433455521E-4	9.610380315029063	6.816597733822084E-4
46	0.0	1.704149433455521E-4	1.704149433455521E-4	10.664226324677959	6.816597733822084E-4
47	0.0	1.704149433455521E-4	1.704149433455521E-4	11.690805943391565	6.816597733822084E-4
48	0.0	1.704149433455521E-4	1.704149433455521E-4	12.593493898292953	6.816597733822084E-4
49	0.0	1.704149433455521E-4	1.704149433455521E-4	13.437047867853437	6.816597733822084E-4
50	0.0	1.704149433455521E-4	1.704149433455521E-4	14.297643331748475	6.816597733822084E-4
51	0.0	1.704149433455521E-4	1.704149433455521E-4	15.222655644228132	6.816597733822084E-4
52	0.0	1.704149433455521E-4	1.704149433455521E-4	16.340918502461644	6.816597733822084E-4
53	0.0	1.704149433455521E-4	1.704149433455521E-4	17.527347338033376	6.816597733822084E-4
54	0.0	1.704149433455521E-4	1.704149433455521E-4	18.576080899381903	6.816597733822084E-4
55	0.0	1.704149433455521E-4	1.704149433455521E-4	19.453717857611498	6.816597733822084E-4
56	0.0	1.704149433455521E-4	1.704149433455521E-4	20.30068012603889	6.816597733822084E-4
57	0.0	1.704149433455521E-4	1.704149433455521E-4	21.048801727325866	6.816597733822084E-4
58	0.0	1.704149433455521E-4	1.704149433455521E-4	21.757216646813326	6.816597733822084E-4
59	0.0	1.704149433455521E-4	1.704149433455521E-4	22.426265714387963	6.816597733822084E-4
60	0.0	1.704149433455521E-4	1.704149433455521E-4	23.082022416381648	6.816597733822084E-4
61	0.0	1.704149433455521E-4	1.704149433455521E-4	23.748685674749446	6.816597733822084E-4
62	0.0	1.704149433455521E-4	1.704149433455521E-4	24.428470883754855	6.816597733822084E-4
63	0.0	1.704149433455521E-4	1.704149433455521E-4	25.151882318256725	6.816597733822084E-4
64	0.0	1.704149433455521E-4	1.704149433455521E-4	25.898981429883623	6.816597733822084E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAT	110	0.0	44.543343	22
ACGCTTC	90	1.05501385E-10	38.883728	15
CTCGTAT	55	6.6957855E-6	38.180008	48
TATATCG	50	1.7090856E-4	35.00132	5
ACGAATA	100	1.2576493E-8	31.501188	24
ACGTTGC	45	0.0043095574	31.106981	15
TGCCGTA	45	0.0043095574	31.106981	19
GTAGATC	220	0.0	30.228413	38
CGACGAG	95	2.8217983E-7	29.472284	22
TAGATCT	235	0.0	28.29894	39
CGACGAA	310	0.0	28.224466	22
CGAACTT	50	0.007206209	28.001057	25
ACGATAC	50	0.007206209	28.001057	24
TACTCGA	50	0.007206209	28.001057	5
GACGATC	75	5.5820397E-5	28.001057	23
AAAGCGT	50	0.007206209	28.001057	35
GACGACC	680	0.0	27.790432	70
AGATCTC	230	0.0	27.390005	40
GACGCTT	95	9.397292E-6	25.786053	14
AGCGTTC	70	0.0012255695	25.000942	37
>>END_MODULE