FastQCFastQC Report
Wed 25 May 2016
SRR1779837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586803
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT257664.390911430241495No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG33340.5681634211140706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC20100.3425340361245597No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT7710.13138992131942065TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6940.11826797068181315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGT200.006934968352.52941553
ATCGTAT407.4887066E-752.48913639
CCGTCTT38400.052.42481247
CGTCTTC38700.052.11332748
TATGCCG38700.052.08224543
ATGCCGT38650.051.96854844
TCGTATG39000.051.77133640
GCCGTCT38900.051.7509746
CGTATGC38950.051.74795541
CTCGTAT38700.051.72056239
TGCCGTC39000.051.69482445
TCTCGTA36950.051.42372538
GTATGCC39650.051.09913342
ATCTCGT37500.050.94945537
TAACTCG1100.050.8985636
CTTGAAA38550.050.86721457
CGTATCT37500.050.1096334
TATCTCG37650.050.0029336
GTCTTCT40500.049.88788649
CTGCTTG40250.049.8540154
Produced by FastQC (version 0.11.5)