FastQCFastQC Report
Wed 25 May 2016
SRR1779836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53882
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA150.002213374770.000012
GTCTGCG150.002213374770.0000133
TATGCAG150.002213374770.0000128
TTCTTAA150.002213374770.0000140
ACGCTTC252.8043687E-670.015
GATACAC308.272584E-658.3333363
GACGCTT308.272584E-658.33333614
ACGAAGG308.272584E-658.33333624
TGGACGC252.370559E-455.99999649
AAGGCCT252.370559E-455.99999627
GGTCGCC608.185452E-1152.50000450
CCGACTA608.185452E-1152.50000421
TCACGCT200.006916288852.513
AATCCGA200.006916288852.59
ATCTAGT200.006916288852.531
TGGACAT200.006916288852.537
TAGAACG200.006916288852.539
ATATGCA200.006916288852.527
GGAACTG200.006916288852.533
GATATGC200.006916288852.526