##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779836_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53882
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.41329200846294	27.0	21.0	34.0	12.0	34.0
2	25.686351657325265	32.0	12.0	34.0	12.0	34.0
3	25.292973534761146	31.0	11.0	34.0	11.0	34.0
4	25.953899261348873	31.0	21.0	34.0	12.0	34.0
5	25.36028729445826	31.0	11.0	34.0	11.0	34.0
6	28.04530269848929	34.0	21.0	38.0	11.0	38.0
7	27.93177684569986	34.0	12.0	38.0	12.0	38.0
8	27.375969711592	34.0	11.0	38.0	11.0	38.0
9	28.38660406072529	34.0	21.0	38.0	11.0	38.0
10-11	27.116458186407336	31.5	11.5	37.5	11.0	38.0
12-13	27.535577743959024	33.5	16.0	38.0	11.0	38.0
14-15	26.63425633792361	29.5	11.0	37.0	11.0	38.0
16-17	26.945872462046694	31.0	11.0	37.5	11.0	38.0
18-19	27.27853457555399	33.0	11.0	38.0	11.0	38.0
20-21	26.683057421773505	29.0	11.0	37.5	11.0	38.0
22-23	27.201031884488327	32.5	11.0	38.0	11.0	38.0
24-25	27.42353661705208	33.0	11.0	38.0	11.0	38.0
26-27	26.598520841839573	31.0	11.0	38.0	11.0	38.0
28-29	27.179401284287888	32.5	11.0	38.0	11.0	38.0
30-31	27.341301362235995	34.0	11.0	38.0	11.0	38.0
32-33	27.52358858245796	34.0	11.0	38.0	11.0	38.0
34-35	27.5809917968895	34.0	11.0	38.0	11.0	38.0
36-37	27.22142830629895	33.0	11.0	38.0	11.0	38.0
38-39	27.139044578894623	33.0	11.0	38.0	11.0	38.0
40-41	27.10224193608255	32.5	11.0	38.0	11.0	38.0
42-43	27.323698080991797	33.5	11.0	38.0	11.0	38.0
44-45	27.45463234475335	34.0	11.0	38.0	11.0	38.0
46-47	27.310929438402432	34.0	11.0	38.0	11.0	38.0
48-49	27.01347388738354	32.5	11.0	38.0	11.0	38.0
50-51	26.625579971047845	31.5	11.0	38.0	11.0	38.0
52-53	27.102585278942875	32.0	11.0	38.0	11.0	38.0
54-55	27.100747930663303	33.0	11.0	38.0	11.0	38.0
56-57	27.14967892802791	33.0	11.0	38.0	11.0	38.0
58-59	27.144686537248063	33.5	11.0	38.0	11.0	38.0
60-61	27.255345013176942	33.5	11.0	38.0	11.0	38.0
62-63	27.103327641884118	33.5	11.0	38.0	11.0	38.0
64-65	27.102176979325193	33.0	11.0	38.0	11.0	38.0
66-67	27.21205597416577	32.5	11.0	38.0	11.0	38.0
68-69	26.835984187669354	31.0	11.0	38.0	11.0	38.0
70-71	27.01049515608181	31.0	11.0	38.0	11.0	38.0
72-73	27.027189042722988	31.0	11.0	38.0	11.0	38.0
74-75	26.81334211796147	31.0	11.0	38.0	11.0	38.0
76	26.201031884488327	28.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	28.0
13	360.0
14	1305.0
15	2383.0
16	3149.0
17	3727.0
18	3829.0
19	3398.0
20	2700.0
21	1891.0
22	1374.0
23	1055.0
24	831.0
25	697.0
26	626.0
27	609.0
28	660.0
29	732.0
30	834.0
31	942.0
32	1215.0
33	1561.0
34	2026.0
35	3038.0
36	5141.0
37	9770.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.90171114657956	21.161055640102447	9.895698006755502	41.04153520656249
2	15.049552726327903	22.044467540180392	35.820867822278316	27.085111911213396
3	13.414498348242457	28.28031624661297	32.7307820793586	25.574403325785976
4	10.82365168330797	29.263947143758585	24.30681860361531	35.60558256931814
5	10.374522103856576	31.212649864518767	30.047140046768867	28.365687984855796
6	19.93652679051985	45.0177242441677	13.143779811065126	21.901969154247322
7	19.592412487471695	29.48327703329745	20.05456772708712	30.86974275214373
8	16.877934707967558	40.56903175516416	18.82852953731371	23.724503999554575
9	18.810668349449696	20.475509938937247	17.769446352010988	42.94437535960207
10-11	18.41651045823203	30.78636253967076	23.048941185204434	27.748185816892786
12-13	17.662670279499647	24.368991499944322	25.30900857429197	32.659329646264055
14-15	25.728443636093683	27.499907204632347	19.124197320069783	27.647451839204184
16-17	26.25576496571179	25.39739984967011	19.47328860555107	28.87354657906703
18-19	19.837417989810785	25.1380369521441	23.404571227067304	31.61997383097781
20-21	25.56048402063769	24.504472736720984	20.68779926506069	29.247243977580638
22-23	26.534399881219723	23.852563983593473	19.6590634917689	29.953972643417902
24-25	19.710284796911683	30.508254377743338	19.20175202531528	30.5797088000297
26-27	24.31609814038083	30.435952637244352	20.194127909134778	25.053821313240043
28-29	20.45138179995917	29.040071270810518	22.85305963362349	27.65548729560682
30-31	21.53966074013585	27.253071526669387	23.451245313833933	27.756022419360825
32-33	18.96644519505586	28.90204520990312	23.681377825618945	28.45013176942207
34-35	18.328863482400543	32.24231850703885	22.179121929490808	27.249696081069796
36-37	23.514070389070387	25.792619542619544	22.901507276507278	27.791802791802795
38-39	24.01325253125203	23.734838009150558	25.38583612521229	26.86607333438512
40-41	20.546940972318886	28.40956543526071	23.207408850904297	27.836084741516103
42-43	17.075275602241934	27.833042574514682	25.012991351471737	30.07869047177165
44-45	16.555768998765743	24.694451404549124	28.21719238657349	30.53258721011164
46-47	19.08332482669661	27.105856587384814	24.920424280106534	28.890394305812045
48-49	19.878623659106935	27.276270368583198	24.427452581567128	28.417653390742736
50-51	24.990488210020324	26.663635266933305	20.910162303616335	27.435714219430036
52-53	15.383901474723663	29.798884444403196	24.044770717129627	30.772443363743513
54-55	17.88813304501963	26.57281003777157	22.991471235139624	32.547585682069176
56-57	19.708035971156253	27.638209609013202	21.84904364611658	30.804710773713957
58-59	18.45509893455099	28.278947173033377	22.386494412889334	30.8794594795263
60-61	16.331910154074624	26.440504919250046	23.752552441061816	33.47503248561352
62-63	18.12216942608954	27.41758853663969	22.69285024871928	31.767391788551492
64-65	18.73955819565621	26.16391312418786	22.55801002413217	32.53851865602376
66-67	16.644855909786997	32.426562717527496	19.56285674509258	31.36572462759293
68-69	17.334174678000075	34.25633792361085	18.376266656768493	30.033220741620582
70-71	15.871719683753389	32.439404624921124	19.28102149140715	32.40785419991834
72-73	15.737312435622616	29.911936378905562	19.5707246457504	34.780026539721426
74-75	16.08448717472809	28.773339767623145	22.46464234010171	32.67753071754705
76	14.440815114509483	31.60981403808322	20.340744590030067	33.60862625737723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	0.5
14	1.0
15	2.5
16	5.5
17	8.0
18	10.5
19	18.0
20	21.5
21	20.0
22	30.0
23	56.0
24	98.0
25	124.0
26	145.5
27	195.0
28	243.0
29	263.0
30	303.0
31	416.5
32	552.5
33	615.0
34	713.5
35	884.0
36	1070.5
37	1185.0
38	1350.5
39	1596.5
40	1877.0
41	2208.0
42	2339.0
43	2469.0
44	2774.5
45	2991.0
46	3032.0
47	3077.5
48	3108.0
49	3067.0
50	3041.0
51	3038.5
52	2923.5
53	2620.5
54	2430.0
55	2352.0
56	2172.5
57	1864.0
58	1657.0
59	1547.5
60	1365.5
61	1181.0
62	1069.0
63	972.5
64	780.5
65	644.0
66	562.0
67	521.0
68	466.5
69	382.0
70	323.5
71	295.0
72	268.5
73	234.5
74	192.0
75	157.0
76	143.5
77	122.0
78	97.0
79	80.0
80	71.5
81	57.5
82	50.0
83	48.0
84	43.0
85	31.0
86	19.0
87	14.0
88	13.0
89	11.0
90	9.5
91	7.5
92	6.0
93	5.0
94	4.0
95	4.0
96	4.0
97	7.0
98	5.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0018559073531049328
7	0.007423629412419731
8	0.0018559073531049328
9	0.005567722059314799
10-11	0.0018559073531049328
12-13	0.0
14-15	0.0
16-17	9.279536765524664E-4
18-19	0.0027838610296573997
20-21	0.0
22-23	0.0018559073531049328
24-25	0.0027838610296573997
26-27	0.0
28-29	0.005567722059314799
30-31	0.0
32-33	0.0
34-35	0.004639768382762333
36-37	0.018559073531049332
38-39	0.010207490442077131
40-41	9.279536765524664E-4
42-43	0.0
44-45	0.006495675735867265
46-47	0.004639768382762333
48-49	0.0
50-51	0.0027838610296573997
52-53	0.013919305148286997
54-55	0.010207490442077131
56-57	0.010207490442077131
58-59	0.014847258824839463
60-61	0.022270888237259198
62-63	0.011135444118629599
64-65	0.022270888237259198
66-67	0.017631119854496866
68-69	0.0
70-71	0.0
72-73	9.279536765524664E-4
74-75	0.007423629412419731
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	53882.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.61823985746632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.11054086063956	76.24995360231617
2	5.235337983067947	8.86010170372295
3	2.5902531034785277	6.575479752050778
4	1.173400008773084	3.9716417356445564
5	0.5483177611089178	2.319884191381166
6	0.16888187042154668	0.8574291971344791
7	0.08773084177742685	0.5196540588693813
8	0.05263850506645612	0.35633421179614716
9	0.01315962626661403	0.10021899706766638
>10	0.01973943939992104	0.18930255001670318
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.011135444118629599	0.0
6	0.0	0.0	0.0	0.011135444118629599	0.0
7	0.0	0.0	0.0	0.011135444118629599	0.0
8	0.0	0.0	0.0	0.012991351471734532	0.0
9	0.0	0.0	0.0	0.012991351471734532	0.0
10	0.0	0.0	0.0	0.012991351471734532	0.0
11	0.0	0.0	0.0	0.012991351471734532	0.0
12	0.0	0.0	0.0	0.014847258824839464	0.0
13	0.0	0.0	0.0	0.014847258824839464	0.0
14	0.0	0.0	0.0	0.014847258824839464	0.0
15	0.0	0.0	0.0	0.016703166177944397	0.0
16	0.0	0.0	0.0	0.016703166177944397	0.0
17	0.0	0.0	0.0	0.016703166177944397	0.0
18	0.0	0.0	0.0	0.020414980884154263	0.0
19	0.0	0.0	0.0	0.02969451764967893	0.0
20	0.0	0.0	0.0	0.02969451764967893	0.0
21	0.0	0.0	0.0	0.033406332355888795	0.0
22	0.0	0.0	0.0	0.06681266471177759	0.0
23	0.0	0.0	0.0	0.07052447941798745	0.0
24	0.0	0.0	0.0	0.08537173824282691	0.0
25	0.0	0.0	0.0	0.11321034853940092	0.0
26	0.0	0.0	0.0	0.11692216324561078	0.0
27	0.0	0.0	0.0	0.12248988530492558	0.0
28	0.0	0.0	0.0	0.1633198470732341	0.0
29	0.0	0.0	0.0	0.1911584573698081	0.0
30	0.0	0.0	0.0	0.2245647897256969	0.0
31	0.0	0.0	0.0	0.2579711220815857	0.0
32	0.0	0.0	0.0	0.2932333617905794	0.0
33	0.0	0.0	0.0	0.3359192309119929	0.0
34	0.0	0.0	0.0	0.38417282209272113	0.0
35	0.0	0.0	0.0	0.43613822797965923	0.0
36	0.0	0.0	0.0	0.5512044838721651	0.0
37	0.0	0.0	0.0	0.7460747559481831	0.0
38	0.0	0.0	0.0	0.9836308971456145	0.0
39	0.0	0.0	0.0	1.3158383133513976	0.0
40	0.0	0.0	0.0	1.6814520619130693	0.0
41	0.0	0.0	0.0	2.130581641364463	0.0
42	0.0	0.0	0.0	2.5351694443413386	0.0
43	0.0	0.0	0.0	3.0418321517389852	0.009279536765524664
44	0.0	0.0	0.0	3.518800341486953	0.009279536765524664
45	0.0	0.0	0.0	3.912252700345199	0.009279536765524664
46	0.0	0.0	0.0	4.274154634200661	0.009279536765524664
47	0.0	0.0	0.0	4.7325637504175795	0.009279536765524664
48	0.0	0.0	0.0	5.159422441631714	0.009279536765524664
49	0.0	0.0	0.0	5.543595263724435	0.009279536765524664
50	0.0	0.0	0.0	5.918488549051632	0.009279536765524664
51	0.0	0.0	0.0	6.2970936490850375	0.009279536765524664
52	0.0	0.0	0.0	6.749935043242641	0.009279536765524664
53	0.0	0.0	0.0	7.148955124160202	0.009279536765524664
54	0.0	0.0	0.0	7.514568872721874	0.009279536765524664
55	0.0	0.0	0.0	7.817081771277977	0.009279536765524664
56	0.0	0.0	0.0	8.132586021305816	0.009279536765524664
57	0.0	0.0	0.0	8.483352511042648	0.009279536765524664
58	0.0	0.0	0.0	8.85267807431053	0.009279536765524664
59	0.0	0.0	0.0	9.129208269923165	0.009279536765524664
60	0.0	0.0	0.0	9.470695222894474	0.009279536765524664
61	0.0	0.0	0.0	9.771352214097472	0.009279536765524664
62	0.0	0.0	0.0	10.133254147952934	0.009279536765524664
63	0.0	0.0	0.0	10.491444267102187	0.009279536765524664
64	0.0	0.0	0.0	10.773542184774136	0.009279536765524664
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACA	15	0.0022133747	70.00001	2
GTCTGCG	15	0.0022133747	70.00001	33
TATGCAG	15	0.0022133747	70.00001	28
TTCTTAA	15	0.0022133747	70.00001	40
ACGCTTC	25	2.8043687E-6	70.0	15
GATACAC	30	8.272584E-6	58.333336	3
GACGCTT	30	8.272584E-6	58.333336	14
ACGAAGG	30	8.272584E-6	58.333336	24
TGGACGC	25	2.370559E-4	55.999996	49
AAGGCCT	25	2.370559E-4	55.999996	27
GGTCGCC	60	8.185452E-11	52.500004	50
CCGACTA	60	8.185452E-11	52.500004	21
TCACGCT	20	0.0069162888	52.5	13
AATCCGA	20	0.0069162888	52.5	9
ATCTAGT	20	0.0069162888	52.5	31
TGGACAT	20	0.0069162888	52.5	37
TAGAACG	20	0.0069162888	52.5	39
ATATGCA	20	0.0069162888	52.5	27
GGAACTG	20	0.0069162888	52.5	33
GATATGC	20	0.0069162888	52.5	26
>>END_MODULE