SRR1779836_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779836_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53882
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	17314	32.13317991165881	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	2089	3.8769904606362053	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1272	2.360714153149475	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	527	0.9780631750862997	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	436	0.8091756059537507	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	320	0.5938903529935786	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	299	0.554916298578375	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	194	0.360046026502357	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT	142	0.2635388441409005	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	137	0.25425930737537583	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	135	0.250547492669166	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	117	0.21714116031327715	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	84	0.15589621766081438	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT	80	0.14847258824839465	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG	79	0.1466166808952897	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	76	0.14104895883597493	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	75	0.13919305148286998	No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	73	0.13548123677666013	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	67	0.12434579265803052	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	61	0.1132103485394009	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTAGG	15	0.002205341	70.064125	1
CATGCAA	15	0.0022134918	69.99907	38
TCGATCT	15	0.0022134918	69.99907	34
AACATGG	15	0.0022134918	69.99907	28
ATGCAAG	15	0.0022134918	69.99907	39
CTTTATA	90	0.0	62.221397	2
GATATCG	35	2.9228977E-7	59.999203	36
TATCGTA	35	2.9228977E-7	59.999203	38
TTTATAC	95	0.0	58.946583	3
CAGTCTT	30	8.273239E-6	58.332558	47
ATCGTAT	50	9.531504E-10	55.999256	39
GTAGAGC	25	2.3707145E-4	55.999256	33
GCTCGTA	25	2.3707145E-4	55.999256	38
TTACACA	50	9.531504E-10	55.999256	3
TCTTTAT	95	0.0	55.31378	1
CTATACA	20	0.0068912543	52.54809	1
GAGTGGA	20	0.006916651	52.499306	28
AGATATC	40	7.3514457E-7	52.499306	35
ATATCGT	40	7.3514457E-7	52.499306	37
GTTAATA	20	0.006916651	52.499306	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content