##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779836_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53882
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.5663486878735	34.0	34.0	34.0	34.0	34.0
2	33.682806874280836	34.0	34.0	34.0	34.0	34.0
3	33.783100107642625	34.0	34.0	34.0	34.0	34.0
4	33.567480791358896	34.0	34.0	34.0	34.0	34.0
5	33.62094948220185	34.0	34.0	34.0	34.0	34.0
6	37.347945510560116	38.0	38.0	38.0	37.0	38.0
7	37.38396867228388	38.0	38.0	38.0	37.0	38.0
8	37.28269180802494	38.0	38.0	38.0	37.0	38.0
9	37.2147655989013	38.0	38.0	38.0	36.0	38.0
10-11	37.15098734271185	38.0	38.0	38.0	36.5	38.0
12-13	37.3185293790134	38.0	38.0	38.0	36.5	38.0
14-15	37.33450874132363	38.0	38.0	38.0	37.0	38.0
16-17	37.1644612300954	38.0	38.0	38.0	36.0	38.0
18-19	36.976077354218475	38.0	38.0	38.0	35.5	38.0
20-21	37.10196354997959	38.0	38.0	38.0	35.5	38.0
22-23	37.1029286218032	38.0	38.0	38.0	36.0	38.0
24-25	37.24319809955087	38.0	38.0	38.0	36.0	38.0
26-27	37.133810920158865	38.0	38.0	38.0	36.0	38.0
28-29	36.469089863034036	38.0	37.0	38.0	34.0	38.0
30-31	36.61068631453918	38.0	37.0	38.0	34.0	38.0
32-33	36.77361642106826	38.0	37.0	38.0	35.0	38.0
34-35	36.919852640956165	38.0	38.0	38.0	35.5	38.0
36-37	36.791396013511005	38.0	37.0	38.0	34.5	38.0
38-39	34.68441223414127	37.0	35.0	38.0	25.5	38.0
40-41	36.204446754018036	38.0	37.0	38.0	33.0	38.0
42-43	37.08566868341932	38.0	37.5	38.0	36.0	38.0
44-45	36.89192123529193	38.0	37.5	38.0	35.0	38.0
46-47	37.05705059203444	38.0	38.0	38.0	36.0	38.0
48-49	36.60000556772206	38.0	37.0	38.0	34.0	38.0
50-51	36.66184440072752	38.0	37.0	38.0	34.0	38.0
52-53	36.59527300397164	38.0	37.0	38.0	34.0	38.0
54-55	36.62858654095987	38.0	37.0	38.0	34.0	38.0
56-57	36.50685757766972	38.0	37.0	38.0	34.0	38.0
58-59	36.50764633829479	38.0	37.0	38.0	34.0	38.0
60-61	36.102659515237	38.0	37.0	38.0	33.0	38.0
62-63	35.980123232248246	38.0	37.0	38.0	33.0	38.0
64-65	36.48544968635166	38.0	37.0	38.0	34.0	38.0
66-67	36.097258824839464	38.0	37.0	38.0	32.5	38.0
68-69	35.91369102854385	38.0	37.0	38.0	31.0	38.0
70-71	33.267529044950074	37.5	31.0	38.0	20.0	38.0
72-73	28.534009502245645	35.0	21.5	38.0	11.0	38.0
74-75	27.74437660072009	34.0	19.0	38.0	11.0	38.0
76	27.829961768308525	34.0	19.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	2.0
24	13.0
25	39.0
26	70.0
27	161.0
28	269.0
29	446.0
30	736.0
31	1147.0
32	1836.0
33	2779.0
34	5201.0
35	11433.0
36	10566.0
37	19180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.50046425255339	12.256267409470752	12.371402042711235	53.871866295264624
2	10.578671912698118	11.194833153928956	59.41316209494823	18.813332838424706
3	10.051594224416318	13.85249248357522	58.9566088860844	17.139304405924054
4	7.349393118295534	45.62191455402546	29.254667606993056	17.774024720685944
5	7.444044393303886	25.90661074199176	58.245796369845216	8.403548494859137
6	15.524665008722765	59.65257414349876	14.854682454251883	9.968078393526596
7	14.581864073345457	23.078207935859844	15.201737129282506	47.13819086151219
8	12.62944953787907	59.5560669611373	11.591997327493411	16.222486173490218
9	12.67399131435359	15.179466241045247	11.32845848335251	60.81808396124866
10-11	13.514717345310123	36.41754203630155	32.70943914479789	17.358301473590437
12-13	14.072417504918155	13.985189859322222	36.8870865966371	35.05530603912253
14-15	13.240971010727145	13.258602130581643	16.769978842656176	56.73044801603504
16-17	33.87587691622434	32.880182621283545	12.857726142310977	20.386214320181136
18-19	35.018187892060425	19.35247392450169	13.48687873501355	32.14245944842433
20-21	14.86488994469396	14.125310864481644	13.544411862959802	57.46538732786459
22-23	13.475743290894918	34.81774989792509	13.350469544560337	38.35603726661965
24-25	33.715351565037444	35.1398001132135	12.977793450320618	18.16705487142844
26-27	35.33833191047103	38.531420511488065	13.030325526149737	13.09992205189117
28-29	14.640325154968265	37.394677257711294	14.43895920715638	33.52603838016407
30-31	13.498014179132179	38.84785271519246	32.84306447422144	14.811068631453919
32-33	52.41731932741918	16.841431275750715	18.57113692884451	12.170112467985598
34-35	19.545488289224604	32.884822389666304	35.26966333840615	12.300025982702943
36-37	33.39612486544672	37.01050443561857	16.715229575739578	12.87814112319513
38-39	14.806428863071156	18.75394380312535	34.329646264058496	32.109981069745
40-41	12.594187298170075	13.967558739467725	37.36498274006161	36.07327122230058
42-43	33.49696553516082	13.42309905161374	35.92732131920343	17.152614094022013
44-45	14.144797891689247	33.90278757284436	38.00341486952971	13.948999665936675
46-47	33.17991165880999	15.022642069707882	19.929661111317323	31.867785160164804
48-49	31.80468431015924	12.944025834230356	16.288370884525445	38.96291897108497
50-51	15.91491257378327	13.30883171845417	36.810892081523555	33.965363626239004
52-53	11.654351700579237	13.38463537798901	57.04830684687361	17.912706074558145
54-55	11.20436736361273	13.51060274074349	40.829836223864525	34.455193671779256
56-57	32.04107020182328	13.37845114094209	20.534172561688855	34.04630609554577
58-59	13.18745590255858	14.438894871699654	54.23428274351071	18.13936648223105
60-61	31.85674767465607	33.36520431465013	20.80277741677961	13.97527059391419
62-63	12.717551725098158	59.123945309236724	14.160934903882746	13.99756806178237
64-65	10.892182612972983	61.39753310997058	13.38784374506947	14.322440531986969
66-67	10.85273476662582	61.16557127473915	13.324792989491662	14.656900969143367
68-69	11.089147502644913	60.97314252835162	13.202294114371623	14.735415854631848
70-71	11.420881670533642	57.71600928074246	13.649187935034805	17.213921113689096
72-73	13.15679558934091	48.70195565208513	15.544974428944021	22.59627432962994
74-75	14.35876969910716	44.64945334397565	16.469288883113993	24.522488073803203
76	13.949214834614098	46.27464082860007	15.662471693210083	24.11367264357575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	2.5
16	0.0
17	0.0
18	12.0
19	29.0
20	29.0
21	24.0
22	32.0
23	62.5
24	87.0
25	89.0
26	122.0
27	167.5
28	222.5
29	265.0
30	268.0
31	358.5
32	453.5
33	461.0
34	577.5
35	727.0
36	859.5
37	959.0
38	1112.5
39	4025.0
40	8016.0
41	8436.5
42	7625.0
43	6299.0
44	4047.0
45	2630.0
46	2139.0
47	1885.0
48	1578.0
49	1436.0
50	1347.0
51	1289.0
52	1112.5
53	989.0
54	984.0
55	906.5
56	830.0
57	771.5
58	712.0
59	630.0
60	550.5
61	489.0
62	425.0
63	416.5
64	371.0
65	293.0
66	262.5
67	273.0
68	276.5
69	247.5
70	179.5
71	144.0
72	147.0
73	129.5
74	110.0
75	111.0
76	89.0
77	70.0
78	64.5
79	56.0
80	50.5
81	40.0
82	28.5
83	22.0
84	17.5
85	15.0
86	13.5
87	10.0
88	6.5
89	2.5
90	2.5
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05938903529935785
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0027838610296573997
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0018559073531049328
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014847258824839463
52-53	0.033406332355888795
54-55	0.05196540588693812
56-57	0.042685869121413464
58-59	0.044541776474518395
60-61	0.03526223970899373
62-63	0.02876656397312646
64-65	0.01577521250139193
66-67	0.037118147062098664
68-69	0.009279536765524666
70-71	0.01299135147173453
72-73	0.023198841913811663
74-75	0.016703166177944397
76	0.014847258824839463
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	53882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.04918154485728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.396162110098544	12.373334323150589
2	19.641401792991033	9.840020786162356
3	13.195524931466252	9.916112987639657
4	8.090686819293175	8.106603318362348
5	4.608431503297028	5.771871868156342
6	2.133807512780618	3.207007906165324
7	1.1484033488923464	2.0136594781188526
8	0.5704971475142625	1.1432389295126388
9	0.2741349929613988	0.6180171485839426
>10	0.7853597095650885	3.1568984076314908
>50	0.06668148477439431	1.2007720574588916
>100	0.05927243091057272	3.303515088526781
>500	0.00740905386382159	0.9780631750862997
>1k	0.01481810772764318	6.23770461378568
>5k	0.0	0.0
>10k+	0.00740905386382159	32.13317991165881
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	17314	32.13317991165881	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	2089	3.8769904606362053	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1272	2.360714153149475	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	527	0.9780631750862997	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	436	0.8091756059537507	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	320	0.5938903529935786	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	299	0.554916298578375	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	194	0.360046026502357	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT	142	0.2635388441409005	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	137	0.25425930737537583	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	135	0.250547492669166	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	117	0.21714116031327715	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	84	0.15589621766081438	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT	80	0.14847258824839465	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG	79	0.1466166808952897	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	76	0.14104895883597493	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	75	0.13919305148286998	No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	73	0.13548123677666013	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	67	0.12434579265803052	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	61	0.1132103485394009	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001855907353104933	0.0
2	0.0	0.0	0.0	0.001855907353104933	0.0
3	0.0	0.0	0.0	0.001855907353104933	0.0
4	0.0	0.0	0.0	0.001855907353104933	0.0
5	0.0	0.0	0.0	0.011135444118629599	0.0
6	0.0	0.0	0.0	0.011135444118629599	0.0
7	0.0	0.0	0.0	0.011135444118629599	0.0
8	0.0	0.0	0.0	0.014847258824839464	0.0
9	0.0	0.0	0.0	0.014847258824839464	0.0
10	0.0	0.0	0.0	0.014847258824839464	0.0
11	0.0	0.0	0.0	0.014847258824839464	0.0
12	0.0	0.0	0.0	0.016703166177944397	0.0
13	0.0	0.0	0.0	0.016703166177944397	0.0
14	0.0	0.0	0.0	0.016703166177944397	0.0
15	0.0	0.0	0.0	0.01855907353104933	0.0
16	0.0	0.0	0.0	0.01855907353104933	0.0
17	0.0	0.0	0.0	0.01855907353104933	0.0
18	0.0	0.0	0.0	0.01855907353104933	0.0
19	0.0	0.0	0.0	0.027838610296573994	0.0
20	0.0	0.0	0.0	0.027838610296573994	0.0
21	0.0	0.0	0.0	0.033406332355888795	0.0
22	0.0	0.0	0.0	0.033406332355888795	0.0
23	0.0	0.0	0.0	0.040829961768308526	0.0
24	0.0	0.0	0.0	0.046397683827623326	0.0
25	0.0	0.0	0.0	0.05753312794625292	0.0
26	0.0	0.0	0.0	0.06124494265246279	0.0
27	0.0	0.0	0.0	0.06681266471177759	0.0
28	0.0	0.0	0.0	0.11135444118629598	0.0
29	0.0	0.0	0.0	0.1354812367766601	0.0
30	0.0	0.0	0.0	0.16703166177944398	0.0
31	0.0	0.0	0.0	0.20043799413533275	0.0
32	0.0	0.0	0.0	0.23570023384432648	0.0
33	0.0	0.0	0.0	0.2820979176719498	0.0
34	0.0	0.0	0.0	0.3377751382650978	0.0
35	0.0	0.0	0.0	0.3897405441520359	0.0
36	0.0	0.0	0.0	0.5196540588693812	0.0
37	0.0	0.0	0.0	0.7776251809509669	0.0
38	0.0	0.0	0.0	1.0318844883263427	0.0
39	0.0	0.0	0.0	1.3733714412976503	0.0
40	0.0	0.0	0.0	1.7631119854496864	0.0
41	0.0	0.0	0.0	2.221521101666605	0.0
42	0.0	0.0	0.0	2.6465238855276345	0.0
43	0.0	0.0	0.0	3.1791692958687503	0.0
44	0.0	0.0	0.0	3.665417022382243	0.0
45	0.0	0.0	0.0	4.077428454771538	0.0
46	0.0	0.0	0.0	4.459745369511154	0.0
47	0.0	0.0	0.0	4.934857651906017	0.0
48	0.0	0.0	0.0	5.372851787238781	0.0
49	0.0	0.0	0.0	5.757024609331502	0.0
50	0.0	0.0	0.0	6.131917894658699	0.0
51	0.0	0.0	0.0	6.506811179985895	0.0
52	0.0	0.0	0.0	6.968932110909023	0.0
53	0.0	0.0	0.0	7.371664006532794	0.0
54	0.0	0.0	0.0	7.73542184774136	0.0
55	0.0	0.0	0.0	8.02494339482573	0.0
56	0.0	0.0	0.0	8.338591737500463	0.0
57	0.0	0.0	0.0	8.698637764002822	0.0
58	0.0	0.0	0.0	9.067963327270702	0.0
59	0.0	0.0	0.0	9.350061244942653	0.0
60	0.0	0.0	0.0	9.69897182732638	0.0
61	0.0	0.0	0.0	10.018187892060428	0.0
62	0.0	0.0	0.0	10.396792992093834	0.0
63	0.0	0.0	0.0	10.766118555361716	0.0
64	0.0	0.0	0.0	11.057496009799191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTAGG	15	0.002205341	70.064125	1
CATGCAA	15	0.0022134918	69.99907	38
TCGATCT	15	0.0022134918	69.99907	34
AACATGG	15	0.0022134918	69.99907	28
ATGCAAG	15	0.0022134918	69.99907	39
CTTTATA	90	0.0	62.221397	2
GATATCG	35	2.9228977E-7	59.999203	36
TATCGTA	35	2.9228977E-7	59.999203	38
TTTATAC	95	0.0	58.946583	3
CAGTCTT	30	8.273239E-6	58.332558	47
ATCGTAT	50	9.531504E-10	55.999256	39
GTAGAGC	25	2.3707145E-4	55.999256	33
GCTCGTA	25	2.3707145E-4	55.999256	38
TTACACA	50	9.531504E-10	55.999256	3
TCTTTAT	95	0.0	55.31378	1
CTATACA	20	0.0068912543	52.54809	1
GAGTGGA	20	0.006916651	52.499306	28
AGATATC	40	7.3514457E-7	52.499306	35
ATATCGT	40	7.3514457E-7	52.499306	37
GTTAATA	20	0.006916651	52.499306	2
>>END_MODULE