FastQCFastQC Report
Wed 25 May 2016
SRR1779835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84827
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCA150.00221778670.01
GTTCTTG150.00221778670.01
CTCTCGG207.886413E-570.042
GTGGTAC150.00221778670.048
TAGATCT1400.060.039
TAGTGTA308.313631E-658.33333234
ACGACCT308.313631E-658.33333224
GTAGATC1450.057.93103438
GCGCAGT252.378386E-456.00000458
TCGGCGG252.378386E-456.00000445
CTAGTGT252.378386E-456.00000433
TCGCCGT452.66391E-854.44444752
TTACGCT452.66391E-854.44444713
AGTGTAG452.66391E-854.44444735
CTTACGC452.66391E-854.44444712
ATCTCGT200.006929964352.542
GTGTGTA200.006929964352.534
CATAGGC200.006929964352.553
CGCTATG200.006929964352.525
TCTCGGA200.006929964352.543
Produced by FastQC (version 0.11.5)