##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779835_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84827
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.643085338394616	32.0	21.0	34.0	12.0	34.0
2	27.09884824407323	33.0	21.0	34.0	12.0	34.0
3	26.633819420703315	33.0	21.0	34.0	11.0	34.0
4	27.03741733174579	33.0	21.0	34.0	12.0	34.0
5	27.193146050196283	34.0	21.0	34.0	11.0	34.0
6	29.614462376366017	36.0	21.0	38.0	11.0	38.0
7	29.734318082685938	36.0	21.0	38.0	12.0	38.0
8	29.208058754877573	34.0	21.0	38.0	11.0	38.0
9	29.928619425418795	36.0	23.0	38.0	11.0	38.0
10-11	28.6166138139979	34.0	21.0	38.0	11.0	38.0
12-13	29.13485682624636	35.0	21.0	38.0	11.0	38.0
14-15	28.181669751376333	34.0	21.0	38.0	11.0	38.0
16-17	28.604412510167755	34.5	21.0	38.0	11.0	38.0
18-19	29.087059544720432	35.5	21.0	38.0	11.0	38.0
20-21	28.328450847018047	33.5	21.0	38.0	11.0	38.0
22-23	28.929438740023812	35.0	21.0	38.0	11.0	38.0
24-25	29.17882278048263	35.0	21.0	38.0	11.0	38.0
26-27	28.29895552123734	34.0	21.0	38.0	11.0	38.0
28-29	29.01006754924729	35.0	21.0	38.0	11.0	38.0
30-31	29.300588256097704	36.5	21.0	38.0	11.0	38.0
32-33	29.461733881900813	37.0	21.0	38.0	11.0	38.0
34-35	29.42099803128721	36.5	21.0	38.0	11.0	38.0
36-37	29.05244202907093	35.5	21.0	38.0	11.0	38.0
38-39	28.975703490633876	35.5	21.0	38.0	11.0	38.0
40-41	28.961350749171842	35.5	21.0	38.0	11.0	38.0
42-43	29.26436158298655	36.5	21.0	38.0	11.0	38.0
44-45	29.32387683166916	36.0	21.0	38.0	11.0	38.0
46-47	29.231229443455504	36.0	21.0	38.0	11.0	38.0
48-49	28.91486790762375	35.5	21.0	38.0	11.0	38.0
50-51	28.426874697914577	34.5	21.0	38.0	11.0	38.0
52-53	28.91562238438233	34.5	21.0	38.0	11.0	38.0
54-55	28.98649015054169	35.5	21.0	38.0	11.0	38.0
56-57	29.0139872917821	36.0	21.0	38.0	11.0	38.0
58-59	29.117497966449363	36.0	21.0	38.0	11.0	38.0
60-61	29.093879307296028	36.0	21.0	38.0	11.0	38.0
62-63	29.012472443915264	36.0	21.0	38.0	11.0	38.0
64-65	29.005069140721705	36.0	21.0	38.0	11.0	38.0
66-67	29.04133117993092	36.0	21.0	38.0	11.0	38.0
68-69	28.605685689697857	34.0	21.0	38.0	11.0	38.0
70-71	28.69139542834239	34.0	21.0	38.0	11.0	38.0
72-73	28.79910287997925	34.5	21.0	38.0	11.0	38.0
74-75	28.705082108290995	35.0	21.0	38.0	11.0	38.0
76	27.99065156141323	33.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	51.0
13	556.0
14	1582.0
15	2942.0
16	3893.0
17	4511.0
18	4581.0
19	4150.0
20	3214.0
21	2333.0
22	1670.0
23	1330.0
24	1167.0
25	1105.0
26	1047.0
27	1073.0
28	1233.0
29	1304.0
30	1569.0
31	1868.0
32	2321.0
33	2915.0
34	4032.0
35	5727.0
36	9787.0
37	18864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.246315988022538	20.134156980171174	9.802419069624879	38.81710796218141
2	16.484138305020807	21.424782203779458	34.73893925283223	27.3521402383675
3	20.34375847312766	20.96855953882608	31.855423391137254	26.832258596909004
4	13.233993893453736	25.55790019687128	29.91146686786047	31.296639041814515
5	14.069812677567285	30.85809942589034	35.75866174684947	19.313426149692905
6	23.43884468022399	42.30002947244327	15.068670792808724	19.19245505452402
7	21.196901637604782	34.35550996828541	17.201334606632948	27.246253787476864
8	18.84608129774592	38.74493067999622	15.837498821088372	26.57148920116948
9	21.527941523225653	18.7844847913228	17.131572742277765	42.55600094317378
10-11	20.472389889653872	29.3719937753466	22.54432707724229	27.61128925775724
12-13	20.10326900633053	23.83439235149186	24.98202223348698	31.080316408690628
14-15	25.472732416947636	26.347464220875676	20.32926225449744	27.85054110767925
16-17	26.30176712603298	25.425866764119913	20.17282233251205	28.099543777335047
18-19	20.72772498997949	25.498667861268952	23.96435055289652	29.809256595855043
20-21	25.37340705199995	24.493970080281045	21.80555719287491	28.327065674844093
22-23	26.420432775907905	24.508249385495937	20.53003554397609	28.541282294620068
24-25	21.178897730621866	29.3964043619216	19.892130857648098	29.532567049808428
26-27	23.95831491338202	29.29096449812264	21.419603543703914	25.33111704479143
28-29	21.360951628685616	28.164530846586423	23.562005588107	26.912511936620966
30-31	22.577260643784665	26.749895374676548	23.40718997011547	27.26565401142332
32-33	20.425689933629624	28.654791516851947	23.53495938793073	27.384559161587703
34-35	19.85911756904123	29.955495299006753	23.03103539744761	27.154351734504406
36-37	23.835941092546957	25.55918453974131	23.53409345485845	27.070780912853287
38-39	24.30456900667873	23.791727333282246	25.250673473117075	26.65303018692195
40-41	21.450843781129716	27.489051180939917	23.839837786540762	27.2202672513896
42-43	18.503062132706173	26.78585111963832	25.252721732005917	29.45836501564959
44-45	18.533560468968282	24.617298068364683	27.542160578606417	29.306980884060618
46-47	20.109518953616544	26.347030079398294	25.548331574821248	27.995119392163915
48-49	20.54004031735179	27.007910217265728	24.71088214837257	27.741167317009914
50-51	24.742116121426466	26.483348069554967	21.997052755673447	26.777483053345126
52-53	17.3208201186083	28.48662414374477	24.650718605939847	29.541837131707087
54-55	19.2946363834641	26.115430639637356	23.901369346215287	30.68856363068326
56-57	20.568174449245717	27.180762950168308	22.821300351940387	29.42976224864559
58-59	19.767503551701573	27.62369058636972	22.999109864003724	29.60969599792498
60-61	17.858890533154888	26.18372412491967	23.980473194861244	31.9769121470642
62-63	19.756899824335957	26.314827695971516	23.40163402930878	30.526638450383746
64-65	19.97122963818912	25.71645865144825	23.124178305496436	31.188133404866203
66-67	18.327054925749724	30.375702267890514	21.035907775203533	30.26133503115623
68-69	19.036981149869735	32.08235585367866	19.74430311103776	29.136359885413842
70-71	18.000271141683317	30.41207641479962	20.473554844299834	31.114097599217228
72-73	17.622508709569033	28.422963788235155	20.790964448452907	33.163563053742905
74-75	17.596571583520493	27.957274479636407	23.079326342098902	31.366827594744194
76	16.26091979769638	29.77022741473421	21.161713214576235	32.80713957299317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.5
12	1.0
13	2.0
14	4.5
15	6.0
16	13.0
17	20.0
18	25.0
19	44.0
20	79.0
21	100.0
22	121.0
23	168.0
24	235.0
25	276.0
26	307.0
27	412.5
28	536.0
29	585.0
30	657.0
31	815.5
32	1103.5
33	1305.0
34	1396.5
35	1625.0
36	1936.0
37	2110.0
38	2233.0
39	2532.5
40	2912.5
41	3315.0
42	3514.0
43	3612.0
44	3874.5
45	4111.5
46	4184.0
47	4251.5
48	4294.5
49	4288.5
50	4307.0
51	4269.0
52	4053.5
53	3784.0
54	3692.0
55	3600.5
56	3334.5
57	2988.5
58	2817.0
59	2635.0
60	2283.5
61	1969.5
62	1825.0
63	1714.0
64	1454.0
65	1191.5
66	1043.0
67	1008.0
68	888.5
69	756.0
70	684.0
71	625.0
72	575.0
73	491.5
74	408.0
75	358.0
76	350.0
77	304.5
78	244.0
79	221.0
80	206.5
81	173.0
82	123.0
83	92.0
84	93.5
85	81.0
86	60.0
87	53.0
88	44.0
89	29.0
90	17.0
91	12.5
92	14.0
93	13.5
94	10.0
95	6.0
96	5.0
97	6.5
98	6.5
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011788699352800404
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002357739870560081
7	0.009430959482240323
8	0.0035366098058401216
9	0.008252089546960283
10-11	0.0035366098058401216
12-13	0.0
14-15	0.0011788699352800404
16-17	0.0
18-19	0.0011788699352800404
20-21	0.0
22-23	0.0029471748382001012
24-25	0.002357739870560081
26-27	5.894349676400202E-4
28-29	0.004715479741120162
30-31	5.894349676400202E-4
32-33	0.0
34-35	0.0053049147087601825
36-37	0.018861918964480646
38-39	0.0064837846440402224
40-41	5.894349676400202E-4
42-43	5.894349676400202E-4
44-45	0.0029471748382001012
46-47	0.0017683049029200608
48-49	0.0
50-51	0.002357739870560081
52-53	0.011788699352800405
54-55	0.0064837846440402224
56-57	0.013557004255720467
58-59	0.010020394449880345
60-61	0.024166833673240833
62-63	0.007073219611680243
64-65	0.02063022386740071
66-67	0.014735874191000508
68-69	0.0
70-71	5.894349676400202E-4
72-73	0.007662654579320263
74-75	0.007662654579320263
76	0.004715479741120162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	84827.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.67124854114846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.73992781049525	69.97418274841736
2	7.0158261606581815	11.31950911855895
3	3.6343177799535296	8.795548587124383
4	1.5300083295582412	4.93710728895281
5	0.6532127252268709	2.6347743053508905
6	0.24696409522000262	1.1953741143739611
7	0.09644751647645072	0.5446379100993788
8	0.04530110622378746	0.2923597439494501
9	0.02484254212272216	0.1803671000978462
>10	0.013151934064970554	0.12613908307496433
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008252089546960283	0.0
2	0.0	0.0	0.0	0.008252089546960283	0.0
3	0.0	0.0	0.0	0.008252089546960283	0.0
4	0.0	0.0	0.0	0.02004078889976069	0.0
5	0.0	0.0	0.0	0.02357739870560081	0.0
6	0.0011788699352800406	0.0	0.0	0.02357739870560081	0.0
7	0.0011788699352800406	0.0	0.0	0.024756268640880853	0.0
8	0.0011788699352800406	0.0	0.0	0.024756268640880853	0.0
9	0.0011788699352800406	0.0	0.0	0.025935138576160893	0.0
10	0.0011788699352800406	0.0	0.0	0.027114008511440933	0.0
11	0.0011788699352800406	0.0	0.0	0.028292878446720973	0.0
12	0.0011788699352800406	0.0	0.0	0.029471748382001013	0.0
13	0.0011788699352800406	0.0	0.0	0.03418722812312117	0.0
14	0.0011788699352800406	0.0	0.0	0.03536609805840122	0.0
15	0.0011788699352800406	0.0	0.0	0.03536609805840122	0.0
16	0.0011788699352800406	0.0	0.0	0.03536609805840122	0.0
17	0.0011788699352800406	0.0	0.0	0.03536609805840122	0.0
18	0.0011788699352800406	0.0	0.0	0.03890270786424134	0.0
19	0.0011788699352800406	0.0	0.0	0.04479705754064154	0.0
20	0.0011788699352800406	0.0	0.0	0.04479705754064154	0.0
21	0.0011788699352800406	0.0	0.0	0.04479705754064154	0.0
22	0.0011788699352800406	0.0	0.0	0.06719558631096231	0.0
23	0.0011788699352800406	0.0	0.0	0.06837445624624235	0.0
24	0.0011788699352800406	0.0	0.0	0.08016315559904276	0.0
25	0.0011788699352800406	0.0	0.0	0.08487863534016292	0.0
26	0.0011788699352800406	0.0	0.0	0.08487863534016292	0.0
27	0.0011788699352800406	0.0	0.0	0.09902507456352341	0.0
28	0.0011788699352800406	0.0	0.0	0.11317151378688389	0.0
29	0.0011788699352800406	0.0	0.0	0.14617987197472504	0.0
30	0.0011788699352800406	0.0	0.0	0.16032631119808552	0.0
31	0.0011788699352800406	0.0	0.0	0.18744031970952646	0.0
32	0.0011788699352800406	0.0	0.0	0.22044867789736758	0.0
33	0.0011788699352800406	0.0	0.0	0.25935138576160893	0.0
34	0.0011788699352800406	0.0	0.0	0.319473752460891	0.0
35	0.0011788699352800406	0.0	0.0	0.3925636884482535	0.0
36	0.0011788699352800406	0.0	0.0	0.5481745199052188	0.0
37	0.0011788699352800406	0.0	0.0	0.8275666945665885	0.0
38	0.0011788699352800406	0.0	0.0	1.1564714065097197	0.0
39	0.0011788699352800406	0.0	0.0	1.5195633465759724	0.0
40	0.0011788699352800406	0.0	0.0	1.9121270350242259	0.0
41	0.0011788699352800406	0.0	0.0	2.3153005528899997	0.0
42	0.0011788699352800406	0.0	0.0	2.7149374609499333	0.0
43	0.0011788699352800406	0.0	0.0	3.116932108880427	0.0
44	0.0011788699352800406	0.0	0.0	3.610878611762764	0.0
45	0.0011788699352800406	0.0	0.0	4.0812477159395	0.0
46	0.0011788699352800406	0.0	0.0	4.555153429922076	0.0
47	0.0011788699352800406	0.0	0.0	4.95125372817617	0.0
48	0.0011788699352800406	0.0	0.0	5.427517182029307	0.0
49	0.0011788699352800406	0.0	0.0	5.843658269183161	0.0
50	0.0011788699352800406	0.0	0.0	6.281019015172056	0.0
51	0.0011788699352800406	0.0	0.0	6.66768835394391	0.0
52	0.0011788699352800406	0.0	0.0	7.141594067926485	0.0
53	0.0011788699352800406	0.0	0.0	7.516474707345538	0.0
54	0.0011788699352800406	0.0	0.0	7.884282127152911	0.0
55	0.0011788699352800406	0.0	0.0	8.228512148254683	0.0
56	0.0011788699352800406	0.0	0.0	8.671767243919978	0.0
57	0.0011788699352800406	0.0	0.0	9.00774517547479	0.0
58	0.0011788699352800406	0.0	0.0	9.349617456706001	0.0
59	0.0011788699352800406	0.0	0.0	9.803482381788816	0.0
60	0.0011788699352800406	0.0	0.0	10.13828144340835	0.0
61	0.0011788699352800406	0.0	0.0	10.495479033798201	0.0
62	0.0011788699352800406	0.0	0.0	10.831456965353013	0.0
63	0.0011788699352800406	0.0	0.0	11.117922359626062	0.0
64	0.0011788699352800406	0.0	0.0	11.475119950015914	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCA	15	0.002217786	70.0	1
GTTCTTG	15	0.002217786	70.0	1
CTCTCGG	20	7.886413E-5	70.0	42
GTGGTAC	15	0.002217786	70.0	48
TAGATCT	140	0.0	60.0	39
TAGTGTA	30	8.313631E-6	58.333332	34
ACGACCT	30	8.313631E-6	58.333332	24
GTAGATC	145	0.0	57.931034	38
GCGCAGT	25	2.378386E-4	56.000004	58
TCGGCGG	25	2.378386E-4	56.000004	45
CTAGTGT	25	2.378386E-4	56.000004	33
TCGCCGT	45	2.66391E-8	54.444447	52
TTACGCT	45	2.66391E-8	54.444447	13
AGTGTAG	45	2.66391E-8	54.444447	35
CTTACGC	45	2.66391E-8	54.444447	12
ATCTCGT	20	0.0069299643	52.5	42
GTGTGTA	20	0.0069299643	52.5	34
CATAGGC	20	0.0069299643	52.5	53
CGCTATG	20	0.0069299643	52.5	25
TCTCGGA	20	0.0069299643	52.5	43
>>END_MODULE