FastQCFastQC Report
Wed 25 May 2016
SRR1779835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84827
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2067924.37785139165596No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG25362.989614155870183No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC16401.9333466938592665No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT5910.696712131750504Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5640.6648826434979428No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT3540.4173199570891344No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT3490.41142560741273415No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2590.30532731323753054No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1730.20394449880344703No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1640.19333466938592667No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1510.17800936022728614No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1320.15561083145696536No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1020.12024473339856413No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1020.12024473339856413No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1000.11788699352800407No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC940.11081377391632381No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG880.10374055430464356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCA150.002219045569.98997567
CTGATAA150.002219045569.98997512
ACTCGTA700.064.9906938
GAACTCG700.059.9914136
AACTCGT800.056.8668637
ATCTCGT29850.053.1079737
TCTCGTA29850.052.99073838
CTCGTAT31850.052.95944239
GCCGTCT32250.052.9536646
TGCCGTC32250.052.9536645
CGTCTTC32200.052.927248
GATCTCG29850.052.873536
CCGTCTT32250.052.8451547
ATGCCGT32200.052.8185244
TATGCCG32400.052.8165143
TCGTATG32550.052.78813640
CTGCTTG32150.052.74516754
CGTATGC32450.052.73512641
AGATCTC29750.052.69833435
TGCTTGA32000.052.66394855