##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779834_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92110
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.608099012050808	32.0	21.0	34.0	12.0	34.0
2	26.614167842796657	32.0	21.0	34.0	12.0	34.0
3	26.573585929866464	33.0	21.0	34.0	11.0	34.0
4	26.69795896211052	32.0	21.0	34.0	12.0	34.0
5	26.935262186516123	34.0	21.0	34.0	11.0	34.0
6	29.120529801324505	36.0	21.0	38.0	11.0	38.0
7	29.562056237107807	36.0	21.0	38.0	12.0	38.0
8	28.934513082184345	35.0	21.0	38.0	11.0	38.0
9	29.862935620453804	36.0	22.0	38.0	11.0	38.0
10-11	28.583573987623495	34.0	21.0	38.0	11.0	38.0
12-13	29.144457713603302	35.5	21.0	38.0	11.0	38.0
14-15	28.207453045271958	34.0	21.0	38.0	11.0	38.0
16-17	28.575833242861798	35.0	21.0	38.0	11.0	38.0
18-19	29.044088589729668	35.5	21.0	38.0	11.0	38.0
20-21	28.307963304744327	33.5	21.0	38.0	11.0	38.0
22-23	28.899169471284335	35.0	21.0	38.0	11.0	38.0
24-25	29.19981000977093	35.5	21.0	38.0	11.0	38.0
26-27	28.288171751167084	34.0	21.0	38.0	11.0	38.0
28-29	28.99992400390837	35.5	21.0	38.0	11.0	38.0
30-31	29.27513842145261	37.0	21.0	38.0	11.0	38.0
32-33	29.445098252089892	37.0	21.0	38.0	11.0	38.0
34-35	29.42041580718706	36.5	21.0	38.0	11.0	38.0
36-37	29.042411247421562	35.5	21.0	38.0	11.0	38.0
38-39	28.951259363804148	35.5	21.0	38.0	11.0	38.0
40-41	28.906307675605255	35.5	21.0	38.0	11.0	38.0
42-43	29.199820866355445	36.5	21.0	38.0	11.0	38.0
44-45	29.311643686896105	36.0	21.0	38.0	11.0	38.0
46-47	29.2110574313321	36.0	21.0	38.0	11.0	38.0
48-49	28.915258929540766	35.5	21.0	38.0	11.0	38.0
50-51	28.44189555965693	35.0	21.0	38.0	11.0	38.0
52-53	28.95399522310281	35.0	21.0	38.0	11.0	38.0
54-55	28.985745304527196	35.5	21.0	38.0	11.0	38.0
56-57	29.05146021061774	36.0	21.0	38.0	11.0	38.0
58-59	29.10939094560851	36.0	21.0	38.0	11.0	38.0
60-61	29.086125284985343	36.0	21.0	38.0	11.0	38.0
62-63	29.033801975898385	36.0	21.0	38.0	11.0	38.0
64-65	29.03177722288568	36.0	21.0	38.0	11.0	38.0
66-67	29.08535989577679	36.0	21.0	38.0	11.0	38.0
68-69	28.653609814352407	34.5	21.0	38.0	11.0	38.0
70-71	28.76799478883943	34.5	21.0	38.0	11.0	38.0
72-73	28.90919009879492	35.0	21.0	38.0	11.0	38.0
74-75	28.717055694278578	35.0	21.0	38.0	11.0	38.0
76	28.088589729671046	34.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	74.0
13	700.0
14	2126.0
15	3416.0
16	4390.0
17	4921.0
18	5179.0
19	4411.0
20	3480.0
21	2431.0
22	1779.0
23	1268.0
24	1087.0
25	1026.0
26	992.0
27	1026.0
28	1084.0
29	1267.0
30	1442.0
31	1804.0
32	2388.0
33	3094.0
34	4105.0
35	6256.0
36	10643.0
37	21721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.45814786668114	21.12799913147324	10.224731299533167	38.18912170231245
2	20.58625556399957	21.826077516013463	32.10509173813918	25.48257518184779
3	14.959287808055585	26.621430897839538	32.615351210509175	25.8039300835957
4	16.503094126587776	27.307567039409403	27.227228313972425	28.9621105200304
5	13.807404190641623	33.23417652806427	34.77581152969276	18.182607751601346
6	26.562075891645403	40.3344009554313	15.124043211552033	17.97947994137126
7	21.671389639859722	33.085784393559386	17.906039976982292	27.3367859895986
8	19.236490374697343	40.55873443285089	18.91836136415457	21.286413828297196
9	22.09904559224313	19.056667281946602	18.198894667694546	40.64539245811572
10-11	20.326911280122033	30.27865088025015	23.673653295984455	25.72078454364336
12-13	19.877863424166758	24.31006405384866	25.55205732276626	30.260015199218326
14-15	25.25906665436247	27.195349013945357	20.75247395762652	26.793110374065648
16-17	26.34093117430884	26.166682046911554	20.59993811713233	26.89244866164728
18-19	20.529927149945173	26.130478682401986	24.48836679079766	28.85122737685518
20-21	24.762240799044623	25.026055802844425	22.42047551840191	27.791227879709048
22-23	26.672637950221205	24.969193605298155	20.946719865374696	27.411448579105937
24-25	20.417447004858452	30.36343403088782	20.875607306679694	28.343511657574027
26-27	23.795306622081565	30.004831258787192	21.699951687412128	24.499910431719112
28-29	20.83525598918608	29.134614862627373	24.005059525425203	26.02506962276135
30-31	22.13506750118066	27.611158458139496	23.929127831548318	26.324646209131526
32-33	19.849093475192706	29.23623927912279	24.11627402019325	26.798393225491264
34-35	19.29745516133235	31.06733399921831	23.76395101402701	25.87125982542233
36-37	23.499071590674646	26.187115198766463	24.15601620101419	26.157797009544698
38-39	23.838607577756424	24.83482358561757	25.993365798574352	25.33320303805165
40-41	20.778212651383964	28.66890677841893	24.448340815451342	26.10453975474576
42-43	18.06989544995603	28.14708660391493	25.66361593329642	28.119402012832623
44-45	18.02986792319677	25.21782086846062	28.296355809370777	28.45595539897183
46-47	19.587438590777083	26.948945525608664	25.964226583068694	27.499389300545555
48-49	20.166105743133212	27.755943980023883	25.344696558462708	26.733253718380194
50-51	24.387132496634386	27.272875320276196	22.353107221956833	25.986884961132585
52-53	16.925440548962552	29.45733488235741	24.675084961075342	28.942139607604695
54-55	18.622527916962984	26.937629948916165	24.348151807477457	30.091690326643395
56-57	19.816929165920158	28.031771367764986	23.27692449685381	28.874374969461048
58-59	18.889323213568304	28.269107571364977	24.19894241940563	28.642626795661098
60-61	17.35424109809202	26.8854452854366	24.495314213732666	31.264999402738713
62-63	18.787865627239363	27.438600681852726	23.715011617554453	30.058522073353455
64-65	19.391129185511762	26.725378304565716	23.50239172100751	30.38110078891501
66-67	17.71083879444677	31.419636118817902	21.152018937892617	29.717506148842716
68-69	18.259245788979424	33.271269521602	20.054934615864813	28.414550073553755
70-71	17.213395072116036	31.481893636309355	21.082744806396803	30.22196648517781
72-73	17.124105659721845	29.618270055478952	20.902862974583908	32.35476131021529
74-75	17.11191335740072	28.995412719524445	23.029233734154882	30.863440188919956
76	16.076042820228867	30.597355221158228	21.783597159794148	31.543004798818753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	1.5
11	0.0
12	0.0
13	0.5
14	1.5
15	4.0
16	12.5
17	19.0
18	24.5
19	38.0
20	60.0
21	74.0
22	95.0
23	124.0
24	200.5
25	269.0
26	350.0
27	431.0
28	512.5
29	594.0
30	700.0
31	895.0
32	1147.5
33	1311.0
34	1525.0
35	1857.0
36	2151.0
37	2327.0
38	2537.5
39	3046.5
40	3663.0
41	4171.0
42	4361.0
43	4557.0
44	4973.0
45	5228.5
46	5264.0
47	5332.0
48	5455.0
49	5325.0
50	5140.0
51	4930.0
52	4476.0
53	4066.0
54	3900.0
55	3723.0
56	3292.5
57	2860.0
58	2681.0
59	2502.0
60	2067.5
61	1699.0
62	1586.0
63	1414.0
64	1156.0
65	1018.5
66	851.0
67	735.0
68	637.0
69	524.5
70	453.0
71	396.0
72	388.0
73	335.5
74	268.5
75	246.0
76	234.5
77	197.0
78	153.5
79	136.0
80	112.0
81	71.5
82	57.5
83	60.0
84	50.0
85	36.5
86	31.0
87	29.0
88	22.0
89	14.0
90	13.5
91	8.0
92	2.0
93	3.5
94	5.5
95	6.0
96	6.0
97	4.5
98	2.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.005428292259255238
7	0.007599609162957334
8	0.009770926066659429
9	0.011942242970361524
10-11	0.005971121485180762
12-13	0.0
14-15	5.428292259255238E-4
16-17	5.428292259255238E-4
18-19	0.003256975355553143
20-21	0.0
22-23	0.002714146129627619
24-25	0.002714146129627619
26-27	0.0016284876777765715
28-29	0.00705677993703181
30-31	5.428292259255238E-4
32-33	0.0
34-35	0.0021713169037020953
36-37	0.01845619368146781
38-39	0.012485072196287049
40-41	0.0016284876777765715
42-43	0.0010856584518510477
44-45	0.0048854630333297145
46-47	0.002714146129627619
48-49	0.0
50-51	0.0021713169037020953
52-53	0.009770926066659429
54-55	0.00705677993703181
56-57	0.01574204755184019
58-59	0.01411355987406362
60-61	0.024970144392574098
62-63	0.008685267614808381
64-65	0.023341656714797526
66-67	0.02117033981109543
68-69	5.428292259255238E-4
70-71	0.0016284876777765715
72-73	0.003256975355553143
74-75	0.008142438388882858
76	0.004342633807404191
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	92110.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.58126153512104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.34230709253036	66.12202800998806
2	6.0718184266859465	9.299750298556074
3	3.4307262649031034	7.881880360438606
4	2.0300826493145636	6.218651612202801
5	0.9484115170331306	3.631527521441755
6	0.4919264520336268	2.260340896753881
7	0.29345468464253815	1.573119096732168
8	0.14885382554331647	0.91195309955488
9	0.07797105147507052	0.5374009336662686
>10	0.16444803583833056	1.5633481706655086
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008685267614808381	0.0
2	0.0	0.0	0.0	0.009770926066659429	0.0
3	0.0	0.0	0.0	0.009770926066659429	0.0
4	0.0	0.0	0.0	0.022798827488872003	0.0
5	0.0	0.0	0.0	0.026055802844425144	0.0
6	0.0	0.0	0.0	0.026055802844425144	0.0
7	0.0	0.0	0.0	0.026055802844425144	0.0
8	0.0	0.0	0.0	0.026055802844425144	0.0
9	0.0	0.0	0.0	0.026055802844425144	0.0
10	0.0	0.0	0.0	0.026055802844425144	0.0
11	0.0	0.0	0.0	0.02822711974812724	0.0
12	0.0	0.0	0.0	0.02822711974812724	0.0
13	0.0	0.0	0.0	0.030398436651829334	0.0
14	0.0	0.0	0.0	0.034741070459233525	0.0
15	0.0	0.0	0.0	0.034741070459233525	0.0
16	0.0	0.0	0.0	0.034741070459233525	0.0
17	0.0	0.0	0.0	0.034741070459233525	0.0
18	0.0	0.0	0.0	0.041255021170339815	0.0
19	0.0	0.0	0.0	0.045597654977744005	0.0
20	0.0	0.0	0.0	0.05536858104440343	0.0
21	0.0	0.0	0.0	0.05971121485180762	0.0
22	0.0	0.0	0.0	0.07708175008142439	0.0
23	0.0	0.0	0.0	0.08251004234067963	0.0
24	0.0	0.0	0.0	0.08576701769623277	0.0
25	0.0	0.0	0.0	0.08793833459993486	0.0
26	0.0	0.0	0.0	0.0955379437628922	0.0
27	0.0	0.0	0.0	0.10856584518510477	0.0
28	0.0	0.0	0.0	0.11942242970361525	0.0
29	0.0	0.0	0.0	0.13462164802952992	0.0
30	0.0	0.0	0.0	0.159591792422104	0.0
31	0.0	0.0	0.0	0.19541852133318857	0.0
32	0.0	0.0	0.0	0.24210183476278363	0.0
33	0.0	0.0	0.0	0.28118553902942134	0.0
34	0.0	0.0	0.0	0.33329714471827165	0.0
35	0.0	0.0	0.0	0.4125502117033981	0.0
36	0.0	0.0	0.0	0.5482575181847791	0.0
37	0.0	0.0	0.0	0.7632178916512865	0.0
38	0.0	0.0	0.0	0.9944631418955596	0.0
39	0.0	0.0	0.0	1.342959504939746	0.0
40	0.0	0.0	0.0	1.7229399630876125	0.0
41	0.0	0.0	0.0	2.234285093909456	0.0
42	0.0	0.0	0.0	2.7163174465313213	0.0
43	0.0	0.0	0.0	3.144066876560634	0.0
44	0.0	0.0	0.0	3.579415915752904	0.0
45	0.0	0.0	0.0	4.109217240256215	0.0
46	0.0	0.0	0.0	4.661817392248398	0.0
47	0.0	0.0	0.0	5.1188796004776895	0.0
48	0.0	0.0	0.0	5.607425903810661	0.0
49	0.0	0.0	0.0	5.985235045054826	0.0
50	0.0	0.0	0.0	6.39344262295082	0.0
51	0.0	0.0	0.0	6.863532732602323	0.0
52	0.0	0.0	0.0	7.252198458364998	0.0
53	0.0	0.0	0.0	7.716860275757247	0.0
54	0.0	0.0	0.0	8.14569536423841	0.0
55	0.0	0.0	0.0	8.566930843556618	0.0
56	0.0	0.0	0.0	8.977309738356313	0.0
57	0.0	0.0	0.0	9.361632830311583	0.0
58	0.0	0.0	0.0	9.732928020844643	0.0
59	0.0	0.0	0.0	10.039083704266638	0.0
60	0.0	0.0	0.0	10.423406796221908	0.0
61	0.0	0.0	0.0	10.788188036043861	0.0
62	0.0	0.0	0.0	11.09760069482141	0.0
63	0.0	0.0	0.0	11.506893931169254	0.0
64	0.0	0.0	0.0	11.84453370969493	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTGA	15	0.002218394	70.00001	57
GACTCGC	15	0.002218394	70.00001	11
ACGCGCG	15	0.002218394	70.00001	24
GTAGATC	150	0.0	53.666668	38
CGGTGGT	60	8.185452E-11	52.500004	46
TAGCGGG	20	0.006931851	52.5	52
GATCCTA	20	0.006931851	52.5	1
CGACCTA	20	0.006931851	52.5	29
GTCTATG	20	0.006931851	52.5	31
CCGCTCG	20	0.006931851	52.5	43
GCTCTCG	20	0.006931851	52.5	41
AGTGCTC	20	0.006931851	52.5	54
CCGTAAC	20	0.006931851	52.5	55
TCCGTGG	20	0.006931851	52.5	45
GTATATC	20	0.006931851	52.5	30
ATTGGGG	20	0.006931851	52.5	34
CTTTCGT	20	0.006931851	52.5	17
GACGCGG	20	0.006931851	52.5	14
GATCTGC	20	0.006931851	52.5	44
GGTTAGA	20	0.006931851	52.5	1
>>END_MODULE